Effect of Conventional Humid–Dry Heating through the Maillard Reaction on Chemical Changes and Enhancement of In Vitro Bioactivities from Soy Protein Isolate Hydrolysate–Yeast Cell Extract Conjugates

Foods, Год журнала: 2024, Номер 13(3), С. 380 - 380

Опубликована: Янв. 24, 2024

This study investigated the formation of soy protein isolate hydrolysate–yeast cell extract (SPIH-YCE) conjugates through a humid–dry heating process and their impact on bioactivity. The incubation SPIH-YCE samples at 60 °C ~75% humidity for varying durations (0, 5, 10, 15, 20 days) resulted in significant decrease reducing sugars free amino acids, while degree glycation increased by approximately 65.72% after 10 days. SDS-PAGE analysis size exclusion chromatography revealed presence peptides glycoprotein molecules, with an increase distribution larger peptide chains. conjugated (10 exhibited highest antioxidant capacity compared to other different times. A comparative between (day 0) days showed significantly higher anti-inflammatory ACE inhibitory activities subjected process. suggests that could serve as alternative substance potential provide health benefits mitigating or preventing non-communicable diseases (NCDs). research highlights importance Maillard reaction enhancing bioactivity offers insights into alterations chemical structure these conjugates.

Язык: Английский

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Phytochemical analysis, GC–MS profile and determination of antibacterial, antifungal, anti-inflammatory, antioxidant activities of peel and seeds extracts (chloroform and ethyl acetate) of Tamarindus indica L

Saudi Journal of Biological Sciences, Год журнала: 2023, Номер 31(1), С. 103878 - 103878

Опубликована: Ноя. 25, 2023

Tamarindus indica L., is widely used tree in ayurvedic medicine. Here, we aimed to understand the presence of important constituents seeds and peel Tamarind fruits their biological activities. Hence, are for further extraction process by soxhlet method (chloroform ethyl acetate solvents). Results suggest that extract (seeds) consists terpenoids (72.29 ± 0.513 mg/g), phenolic content (68.67 2.11 mg/g) flavonoids (26.36 2.03 whereas chloroform has (42.29 0.98 mg/g). Similarly, (peel) (25.96 3.20 (46.36 (62.93 0.987 Furthermore, anti-inflammation activity results revealed was found be more effective with IC50 226.14 µg/ml protein denaturation analysis 245.5 on lipoxygenase inhibition activity. Chloroform (peel seeds) shown better antioxidant using DPPH than seeds). Ethyl showed impressive potency inhibiting growth fungus, Candida albicans. Additionally, Escherichia coli Bacillus cereus. GC-MS existence diverse set phytochemicals each extract. Overall, comparative studies highlight effectiveness extracts extracts. Moreover, acetate) contains a wide range compounds (including flavonoids, isovanillic acid, fatty acids compounds) which can utilized therapeutic purpose.

Язык: Английский

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Design of Two-Dimensional Phosphorus-Doped Polymeric Graphitic Carbon Nitride Fluorescent Probe for the Highly Sensitive Picomolar Detection of Nitrofurazone in Biological Fluids and Environmental Samples

Опубликована: Янв. 1, 2025

Язык: Английский

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Studies on Carrageenan Induced Inflammation in Wistar Rats Treated with <i>Gongronema latifolium </i>Aqueous Leaves Extracts (Utazi)

American Journal of Biomedical and Life Sciences, Год журнала: 2025, Номер 13(1), С. 22 - 30

Опубликована: Фев. 7, 2025

AIM: The aim of this research study is to evaluate the ameliorative effects aqueous solvent extracts dried leaves <i>Gongronema latifolium </i>preparations on liver enzymes- alkaline phosphatase, gamma glutamyl transferase and lipid profile concentration- total cholesterol, triglycerides, high density lipoprotein, low lipoprotein carrageenan induced inflamed female wistar rats. Materials Method: Inflammation (rat paw oedema) was by injection into sub-plantar region rat right hind paw. sizes were measured using electronic Vernier caliper after 3 hours for confirmation swelling (oedema) along with behavioural physical changes these rats such as pain, flinching their legs, redness, heat, leaking paws tongues at interval. Forty used study. divided 5 groups 8rats in each group sub two biochemical studies 7<sup>th</sup> day 14<sup>th</sup> day. Results: For enzyme: This showed that serum ALP concentration Negative control decreased significantly (p‹0.05) when compared Normal group. While 20mg/kg ibuprofen increased day; extract 250mg/kg 500mg/kg (p›0.05) negative GGT Ibuprofen (p‹0.05). but not More also Furthermore, TG group, Conclusion: From results my findings study, enzymes profiles parameters a result treatment given wistar. implication suggest latifolium</i> may be novel drug like synthetic (ibuprofen)in management inflammatory diseases affects concentration.

Язык: Английский

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Bioassay-guided identification of potential Alzheimer’s disease therapeutic agents from Kaempferol-Enriched fraction of Aframomum melegueta seeds using in vitro and chemoinformatics approaches

Arabian Journal of Chemistry, Год журнала: 2023, Номер 16(9), С. 105089 - 105089

Опубликована: Июнь 21, 2023

Alzheimer's disease (AD) has become a major public health concern and the fifth cause of death among aging population globally. In this study, total phenols flavonoids contents (TPC TFC) in vitro antioxidant actions methanol extract various fractions Aframomum melegueta were evaluated using 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, nitric oxide activity (NO), lipid peroxidation (TBARS) ferric reducing power assay (FRAP). Furthermore, acetylcholinesterase (AChE) butyrylcholinesterase (BuChE) inhibitory activities two most potent investigated, phytochemicals identified ethyl acetate fraction, which had best cholinesterase effects subjected to chemoinformatics studies. The its fraction high amounts TPC TFC. exerted DPPH, NO, TBARS, FRAP inhibition with IC50 values 5.06, 6.58, 2.12, 88.73 µg/mL, respectively. Interestingly, n-hexane inhibited AChE (IC5016.83 11.67 µg/mL) BuChE (IC50 7.54 and5.21 enzymatic more than standard inhibitor, rivastigmine 11.99 11.40 µg/mL values, A 18 compounds identified, kaempferol was component, 40.01 µg/g (30%). More strikingly, top-scoring (catechin, kaempferol) exhibited good binding affinity, interacted favorably amino acids residues around within active sites also obeyed drug-likeness rules, did not show tendency towards toxicity when placed side by is immunogenic. Thus, A. seeds contain safe bioactive chemicals, could be veritable remedy for managing other neurodegenerative diseases.

Язык: Английский

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Combretum micranthum G. Don (Combretaceae): A Review on Traditional Uses, Phytochemistry, Pharmacology and Toxicology

Chemistry & Biodiversity, Год журнала: 2024, Номер 21(5)

Опубликована: Фев. 14, 2024

Combretum micranthum (Combretaceae) is a medicinal plant widely known and used in Africa to treat variety of conditions such as diabetes, fever, coughs, bronchitis, diarrhea, pain, malaria liver disorders, among others. Due its wide traditional use, this review, published scientific reports on composition pharmacological properties were explored by conducting literature search databases. To date, 155 organic compounds including 34 flavonoids, 16 phenolic acids, 14 alkaloids, 15 fatty terpenoids/steroids, 24 amino 8 carbohydrate substances 30 other have been identified from plant. In addition these compounds, 6 minerals (potassium nitrate, calcium, magnesium, potassium, sodium, iron zinc) also reported. vitro vivo studies shown that phytochemicals extracts range potential, antibacterial, antiviral, antioxidant, antidiabetic, anti-inflammatory, analgesic, antihypertensive, nephroprotective, hepatoprotective, anxiolytic, anti-cholinesterase antidiarrheal activities. Additionally, no harmful effects revealed through studies. Thus, study could constitute valuable reference for the valorization C. pharmaceutical industry.

Язык: Английский

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Insights into the in vitro biological properties of Australian beach‐cast brown seaweed phenolics

Food Science & Nutrition, Год журнала: 2024, Номер 12(11), С. 8956 - 8967

Опубликована: Сен. 15, 2024

Abstract Five Australian seaweed species, Phyllosphora comosa , Ecklonia radiata Durvillaea potatorum Sargassum fallax and Cystophora siliquosa thrive along the country's shorelines. Some of these seaweeds have recognized health benefits but not been fully investigated in terms their bioactive components mechanisms action. We employed ultrasonication with 70% methanol to extract phenolic compounds from a range bioactivities for extracts, including anti‐inflammatory activity exploring urease inhibition, nitric oxide scavenging activity, protein denaturation protease inhibition. Anti‐diabetic activities were using α‐amylase α‐glucosidase inhibition assays. Anti‐proliferative anti‐mitotic evaluated yeast‐cell green‐gram models, respectively. Our findings showed that C. inhibited oxide, urease, activities, S. P. E. exhibiting substantial denaturation. displayed inhibitory effects on both α‐glucosidase, whereas targeted only enzyme, indicating different anti‐diabetic activity. In assays, exhibited low cell viability significant anti‐proliferative effect, particularly within 24 h, while demonstrated notable at 48 h. LC‐ESI‐QTOF‐MS/MS investigation identified compounds, 19 acids, 20 flavonoids, 9 other polyphenols. The presence extracts correlated observed biological activities. These results support potential link this phenolics.

Язык: Английский

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Kinkéliba (Combretum micranthum) Leaf Extract Alleviates Skin Inflammation: In Vitro and In Vivo Study

Molecules, Год журнала: 2023, Номер 28(4), С. 1791 - 1791

Опубликована: Фев. 14, 2023

Kinkéliba (Combretum micranthum, Seh-Haw in Wolof) is a popular bush tea West African countries. Although the kinkéliba plant’s leaves have been widely consumed for its nutritional and medicinal properties, benefits on skin health potential practically untouched. In human epidermal primary keratinocytes, vitexin isovitexin-rich extract treatment significantly (p < 0.001) enhanced up to 39.6% of cell survival rate decreased by UV radiation irritation. The leaf extracts also reduced production UV-induced pro-inflammatory cytokines IL-6 IL-8 57.6% 42.5%, respectively 0.001), which cause redness barrier dysfunction, as well wrinkles collagen degradation. anti-inflammation efficacy might involve significant inhibition levels cellular reactive oxygen species (ROS) (−70.8%, p nitrotyrosine (−56.9%, 0.05). Further topical applications gel were found reduce sodium lauryl sulfate (SLS)-induced inflammation: at D7, trans-epidermal water loss (TEWL) (a* value) both 59.81% 22.4% compared with D0. vitro vivo data support new application an effective active ingredient inflammation, subsequent dysfunction inflammaging.

Язык: Английский

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Ex vivo and in vitro investigations on the protective effects of Combretum glutinosum Perr. ex DC against oxidative and inflammatory stress-induced liver and kidney damage

Phytomedicine Plus, Год журнала: 2023, Номер 3(4), С. 100484 - 100484

Опубликована: Авг. 31, 2023

Organ damage, such as liver and kidney injury, is a serious frequent problem leading to end-stage failure. Despite advances in research, there still no proof of the effectiveness potentially anti-inflammatory, antioxidant, organoprotective compounds therapeutic contexts. The need for potential drugs therefore enormous urgent. For long time, plant-based remedies, including Combretum glutinosum, have been crucial component healthcare. In order develop phytomedicine, current research aims evaluate nephroprotective hepatoprotective abilities C. glutinosum extract, targeting its anti-inflammatory antioxidant using ex vivo vitro models. phytochemical screening pointed out large number beneficial substances, flavonoids, tannins, polyphenols, alkaloids, terpenoids. extract showed strong activity against heat hypotonia-induced protein denaturation membrane destabilization. Moreover, remarkable action DPPH radicals, ferric-reducing power (FRAP), Total Antioxidant Capacity (TAC) Ferric chloride/ascorbic acid (FeCl2-AA)-mediated lipid peroxidation tissues was significantly (p < 0.001) mitigated by extract. has good profile. This would place it among candidates activities.

Язык: Английский

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Metabolite Profiling and Molecular Network Shows Kinkeloids as Promoting of Collagen Synthesis from Combretum micranthum

Separations, Год журнала: 2024, Номер 11(5), С. 132 - 132

Опубликована: Апрель 26, 2024

Combretum micranthum, a plant native to Africa, has well-documented traditional use in the treatment of various ailments such as fever, diabetes, and malaria. Its pharmaceutical benefits include nephroprotective, anti-inflammatory, antioxidant, antimicrobial properties, which were proven. In addition, its potential for cosmetic applications is being explored due depigmenting, UV-damage-repairing properties. This article investigates molecular composition new cosmetically relevant biological activity C. micranthum enriched fractions begin establishment structure–activity relationship. Firstly, an extract was prepared selected overall response then fractionated obtain simplified fractions. One fraction particularly kinkeloids, specific family compounds this species. All crude tested on targets evaluate compare their activities. Molecular networks constructed from UHPLC-MS/HRMS data better characterize highlight relationships. study highlights metabolic profiling butylene glycol showing main chemical families revealing that identified by HRMS NMR, promote increase collagen I synthesis, interesting neither previously described these extract.

Язык: Английский

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