LWT,
Год журнала:
2024,
Номер
206, С. 116598 - 116598
Опубликована: Авг. 1, 2024
Eicosapentaenoic
acid
(EPA),
an
orally
active
Omega-3
long-chain
polyunsaturated
fatty
acid,
has
attracted
much
attention
for
its
medical
and
healthcare
value.
Due
to
a
large
amount
of
acids
(PUFA)
including
docosahexanoic
(DHA)
in
fish
oil,
the
highly
specific
enrichment
EPA
from
oil
is
difficult
achieve.
In
our
study,
we
conducted
immobilization
studies
on
Burkholderia
cepacia
lipase
(BCL)
application
selective
oil.
BCL
was
immobilized
resin
LX201A
by
covalent
crosslinking,
conditions
glutaraldehyde
concentration,
pH,
time
were
optimized.
The
optimal
0.5%
glutaraldehyde,
4
h,
pH
8.0.
loading
rate
reached
93.5%,
activity
up
68263
U/g
under
conditions.
After
hydrolysis
reaction
15h
catalyzed
BCL,
content
showed
more
increase
33.2%,
while
slight
0.78%
DHA
content.
Moreover,
after
recycling
use
10
times,
catalytic
efficiency
remained
at
93.0%.
These
indicated
that
possessed
good
selectivity,
reusability
stability,
providing
great
potential
industry.
Foods,
Год журнала:
2023,
Номер
12(23), С. 4258 - 4258
Опубликована: Ноя. 24, 2023
Immobilized
enzymes
are
currently
being
rapidly
developed
and
widely
used
in
juice
clarification.
have
many
advantages,
they
show
great
advantages
The
commonly
methods
for
immobilizing
include
adsorption,
entrapment,
covalent
bonding,
cross-linking.
Different
immobilization
adopted
different
to
accommodate
their
characteristics.
This
article
systematically
reviews
the
of
enzyme
use
immobilized
supports
In
addition,
mechanisms
effects
clarification
with
pectinase,
laccase,
xylanase
fruit
elaborated
upon.
Furthermore,
suggestions
prospects
provided
future
studies
this
area.
Food Reviews International,
Год журнала:
2023,
Номер
40(1), С. 457 - 503
Опубликована: Фев. 14, 2023
Lipase
has
been
used
extensively
in
the
industrial
sector
for
edible
fats
and
oils
processing.
However,
its
scale
application
is
often
hampered
by
limitations
such
as
low
stability
poor
reusability.
A
countermeasure
taken
lipase
immobilization
which
a
technique
to
confine
enzyme
molecules
within
fixed
space
while
maintaining
catalytic
activity.
This
usually
enhances
allows
reusability
together
with
other
advantages
like
simple
product
separation
better
design
options
bioreactor.
Meanwhile,
involves
high
cost
thus
making
them
less
attractive
certain
applications
(e.g.,
monoglyceride
diglyceride
production,
vitamin
C
esters
synthesis)
compared
chemical
catalysts.
Their
lower
activity
free
catalysts
also
challenge
that
needs
be
overcome.
Therefore,
this
paper
will
discuss
important
characteristics
of
lipase,
trending
materials
protocols
immobilization,
current
immobilized
oil
fat
industries.
provide
thorough
understanding
technology
modifications
so
strategy
can
more
appropriately
designed
optimized
benefits.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Фев. 7, 2024
Enzyme
therapy
based
on
differential
metabolism
of
cancer
cells
has
demonstrated
promising
potential
as
a
treatment
strategy.
Nevertheless,
the
therapeutic
benefit
reported
enzyme
drugs
is
compromised
by
their
uncontrollable
activity
and
weak
stability.
Additionally,
thermozymes
with
high
thermal-stability
suffer
from
low
catalytic
at
body
temperature,
preventing
them
functioning
independently.
Langmuir,
Год журнала:
2024,
Номер
40(17), С. 8921 - 8938
Опубликована: Апрель 16, 2024
In
this
work,
a
trimetallic
(Ni/Co/Zn)
organic
framework
(tMOF),
synthesized
by
solvothermal
method,
was
calcinated
at
400
and
600
°C
the
final
products
were
used
as
support
for
lipase
immobilization.
The
material
annealed
(Ni-Co-Zn@400)
had
an
improved
surface
area
(66.01
m2/g)
pore
volume
(0.194
cm3/g),
which
showed
highest
enzyme
loading
capacity
(301
mg/g)
with
specific
activity
of
0.196
U/mg,
could
protect
against
thermal
denaturation
65
°C.
optimal
pH
temperature
8.0
45
but
tolerate
levels
7.0–8.0
temperatures
40–60
Moreover,
immobilized
(Ni-Co-Zn@Lipase,
Ni-Co-Zn@400@Lipase,
or
Ni-Co-Zn@600@Lipase)
be
recovered
reused
over
seven
cycles
maintaining
80,
90,
11%
its
original
maintained
residual
>90%
after
40
storage
days.
remarkable
thermostability
stability
suggest
that
rigid
structure
acted
protective
shield
denaturation,
while
tolerance
toward
alkaline
range
indicates
shift
in
ionization
state
attributed
to
unequal
partitioning
hydroxyl
hydrogen
ions
within
microenvironment
active
site,
suggesting
acidic
residues
may
have
been
involved
forming
enzyme–support
bond.
high
capacity,
activity,
encouraging
stability,
recoverability
tMOF@Lipase
indicate
multimetallic
MOF
better
platform
efficient
