Toxins,
Год журнала:
2023,
Номер
15(4), С. 273 - 273
Опубликована: Апрель 6, 2023
Many
insects,
including
the
Plutella
xylostella
(L.),
have
developed
varying
degrees
of
resistance
to
many
insecticides,
Bacillus
thuringiensis
(Bt)
toxins,
bioinsecticides
derived
from
Bt.
The
polycalin
protein
is
one
potential
receptors
for
Bt
and
previous
studies
confirmed
that
Cry1Ac
toxin
can
bind
P.
xylostella,
but
whether
associated
with
toxins
remains
controversial.
In
this
study,
we
compared
midgut
larvae
Cry1Ac-susceptible
-resistant
strains,
found
expression
Pxpolycalin
gene
was
largely
reduced
in
resistant
strains.
Moreover,
spatial
temporal
patterns
showed
it
mainly
expressed
larval
stage
tissue.
However,
genetic
linkage
experiments
its
transcript
level
were
not
linked
resistance,
whereas
both
PxABCC2
levels
resistance.
fed
on
a
diet
containing
no
significant
change
short
term.
Furthermore,
knockout
ATP-binding
cassette
transporter
subfamily
C2
(ABCC2)
genes
separately
by
CRISPR/Cas9
technology
resulted
decreased
susceptibility
toxin.
Our
results
provide
new
insights
into
role
ABCC2
proteins
mechanism
underlying
insects
toxins.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Янв. 3, 2023
ABSTRACT
Transcription
factor
aryl
hydrocarbon
receptor
(AhR)
can
enhance
insect
resistance
to
insecticides
by
regulating
the
detoxification
metabolic
network.
Our
previous
studies
have
confirmed
that
overexpression
of
cytochrome
P450
monooxygenases
(P450s)
and
glutathione
S-transferases
(GSTs)
are
both
involved
in
lambda
-cyhalothrin
Cydia
pomonella
.
In
this
study,
we
report
AhR
regulates
GSTs
thus
conferring
C.
Spatiotemporal
expression
patterns
indicated
gene
(
CpAhR
)
was
highly
expressed
Malpighian
tubules
larvae.
Moreover,
induced
exposure
up-regulated
a
-cyhalothrin-resistant
population.
RNA
interference
(RNAi)
could
effectively
decrease
relative
level
CpGSTe3
enzyme
activity
GSTs,
but
not
P450s,
further
reducing
tolerance
larvae
-cyhalothrin.
Furthermore,
β-naphthoflavone
(BNF),
novel
agonist
AhR,
increase
enzyme,
resulting
enhancement
These
results
demonstrate
activate
leading
development
-cyhalothrin,
which
enriches
theory
insecticide
regulation
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Ноя. 27, 2024
Migratory
insect
infestation
caused
by
Sogatella
furcifera
is
a
serious
threat
to
rice
production.
The
most
effective
method
available
for
S.
control
intensive
insecticide
spraying,
which
cause
widespread
resistance.
RNA
interference
(RNAi)
insecticides
hold
enormous
potential
in
managing
pest
However,
the
instability
and
poor
efficiency
of
cross-kingdom
trafficking
are
key
obstacles
application
agricultural
management.
We
present
dendritic
mesoporous
silica
nanoparticles
(DMSNs)-based
nanocarrier
delivering
siRNA
nitenpyram
inhibit
metabolic
detoxification
development
furcifera,
thereby
preventing
its
proliferation.
This
nano
complex
(denoted
as
N@UK-siRNA/DMSNs)
significantly
enhanced
stability
(efficacy
lasting
21
days)
released
cargos
GSH
or
planthopper
bodily
fluid
with
maximum
release
rate
84.99%.
Moreover,
UK-siRNA
targeting
two
transcription
factors
(Ultraspiracle
Krüppel-homolog
1)
downregulated
developmental
genes
Ultraspiracle
(0.09-fold)
1
(0.284-fold),
downstream
ABC
SfABCH4
(0.016-fold)
P450
CYP6FJ3
(0.367-fold).
N@UK-siRNA/DMSNs
inhibited
detoxification,
enhancing
susceptibility
nanogram
level
(LC50
250–252
ng/mL),
resulting
5.37–7.13-fold
synergistic
ratio.
work
proposes
comprehensive
management
strategy
controlling
ensure
green
safe
production
rice.
Schematic
fabrication
molecular
mechanism
resolution
improving
sensitivity
nitenpyram.
assembled
onto
DMSNs
interfacial
modification.
With
phloem
xylem
pathway,
could
transport
bi-directionally
feeding
sites
then
response
glutathione
(GSH).
from
would
downregulate
USP
Kr-H1.
releasing
competitively
bind
acetylcholine
(ACh)
receptors,
inhibiting
transmission
nerve
impulses
