The long non-coding RNA MSTRG.32189-PcmiR399b-PcUBC24 module regulates the phosphate accumulation and disease resistance to Botryosphaeria dothidea in pear DOI Creative Commons

Yuekun Yang,

Shamei Lv,

Xiaosan Huang

и другие.

Horticulture Research, Год журнала: 2024, Номер unknown

Опубликована: Дек. 30, 2024

Pear ring rot disease (Botryosphaeria dothidea) is a significant threat to the healthy development of pear industry. Recent research has identified functional role long non-coding RNAs (lncRNAs) in various biological processes plants. The lncRNAs defense response remains unknown. In this study, transcriptome sequencing was used analyze stem infected with B. dothidea. It 3555 lncRNAs, which 286 were significantly differentially expressed. GO and KEGG analyses showed that cis- trans-regulated target genes enriched multiple resistance-related pathways. More specifically, MSTRG.32189, predicted as an endogenous mimic (eTM), down-regulated dothidea infection, confirmed inhibit cleavage effect PcmiR399b on PcUBC24. OE-MSTRG.32189 transgenic Arabidopsis exhibited lower Pi content weaker resistance Botrytis cinerea compared wild type. callus, overexpression MSTRG.32189 negatively regulated PcmiR399b, decreased reduced resistance. Overexpressing callus opposite effects OE-MSTRG.32189. Overexpression knockout PcUBC24 further clarified regulates infection. Furthermore, ROS levels expressions pathway-related by MSTRG.32189-PcmiR399b-PcUBC24 module contributed Overall, our results demonstrated response, revealing phosphate accumulation infection pear.

Язык: Английский

The role of epigenetics in tomato stress adaptation DOI Creative Commons

Marianne Delarue,

Moussa Benhamed, Sotirios Fragkostefanakis

и другие.

New Crops, Год журнала: 2024, Номер 2, С. 100044 - 100044

Опубликована: Авг. 28, 2024

Climate change poses a major challenge to agriculture, affecting crop production through shifting weather patterns and an increase in extreme conditions such as heat waves, droughts, floods, all of which are further compounded by biotic stress factors.Tomatoes, vital dietary staple significant agricultural product worldwide, particularly susceptible these changes.The need for developing climate-resilient tomato varieties is more urgent than ever ensure food security.Epigenetic modifications, DNA methylation histone play essential roles gene expression regulation.These modifications can affect plant traits responses environmental stresses, enabling tomatoes maintain productivity despite variable climates or disease pressures.Tomato, model plant, offers valuable insights into the epigenetic mechanisms underlying fruit development stress.This review provides overview key discoveries regarding response resilience related epigenetics, highlighting their potential breeding strategies enhance against both abiotic challenges, thereby promoting sustainable practices context global climate change.

Язык: Английский

Процитировано

10
Harnessing the potential of non-coding RNA: An insight into its mechanism and interaction in plant biotic stress DOI
Syed Muhammad Iqbal Syed Othman, Arif Faisal Mustaffa, Nur Irdina Izzatie Mohd Zahid

и другие.

Plant Physiology and Biochemistry, Год журнала: 2024, Номер 207, С. 108387 - 108387

Опубликована: Янв. 18, 2024

Язык: Английский

Процитировано

5
Long Noncoding RNA–Messenger RNA (lncRNA–mRNA) Network in Resistance to Gummy Stem Blight (Stagonosporopsis cucurbitacearum) of Melon Plant Introduction (PI) 420145 DOI Creative Commons
Rui Zhang, Jing Chen, Yongbing Zhang

и другие.

Horticulturae, Год журнала: 2025, Номер 11(1), С. 31 - 31

Опубликована: Янв. 2, 2025

Gummy stem blight (GSB), caused by Stagonosporopsis cucurbitacearum (Didymella bryoniae), poses a growing threat to greenhouse melon production. Despite this, the defense mechanism of against S. remains poorly understood. In this study, employing electron microscopy and transcriptome sequencing, we investigated cellular ultrastructure differences gene expression dynamics two accessions, PI 420145 (resistant GSB) ‘Baipicui’ (susceptible), pre- post- inoculation. Our results revealed that exhibits thicker waxy layer on leaf surface limited conidial germination without obvious signs cell damage compared ‘Baipicui’. The transcriptomic analysis identified total 23,078 differentially expressed genes (DEGs) 974 long noncoding RNAs (DELs). Specifically, phenylpropanoid biosynthesis, cyanoamino acid metabolism, MAPK signaling–plant plant–pathogen interactions were enriched in 420145, while displayed enrichment metabolism autophagy. Additionally, through lncRNA–mRNA network construction, 38 targeted regulatory relationships four most significant KEGG pathways for disease resistance.

Язык: Английский

Процитировано

0
Non-coding RNA and hormonal regulation in plants DOI

Vidyut Balar,

Parth Bagadiya,

Riecha Joshi

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 225 - 248

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
The lncRNA20718-miR6022-RLPs module regulates tomato resistance to Phytophthora infestans DOI
Jiahui Zhang, Hongbo Wei, Yuhui Hong

и другие.

Plant Cell Reports, Год журнала: 2024, Номер 43(2)

Опубликована: Фев. 1, 2024

Язык: Английский

Процитировано

3
Regulatory Peptide Encoded by the Primary Transcript of miR396a Influences Gene Expression and Root Development in Solanum lycopersicum DOI
Zhengjie Wang, Ruili Lv,

Chenglin Su

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(29), С. 16390 - 16402

Опубликована: Июль 12, 2024

MicroRNAs (miRNAs) are the processing products of primary miRNAs (pri-miRNAs) that regulate expression target genes. Recent studies have demonstrated some pri-miRNAs can encode small peptides (miPEPs) perform significant biological functions. The function miPEPs in tomatoes, an important model horticultural crop, remains to be investigated. Here, we characterized sequence tomato miR396a using 5' RACE and confirmed presence miPEP396a by verifying translational activity start codon. It primarily resides nucleus exert its additionally regulates pri-miR396a, miR396a, Transcriptomic metabolomic analyses showed

Язык: Английский

Процитировано

2
Transcriptomic and epigenetic responses shed light on soybean resistance to Phytophthora sansomeana DOI Creative Commons
Gwonjin Lee, Charlotte N. DiBiase, Beibei Liu

и другие.

The Plant Genome, Год журнала: 2024, Номер 17(3)

Опубликована: Июль 12, 2024

Phytophthora root rot, caused by oomycete pathogens in the genus, poses a significant threat to soybean productivity. While resistance mechanisms against sojae have been extensively studied soybean, molecular basis underlying immune responses sansomeana remains unclear. In this study, we investigated transcriptomic and epigenetic of two resistant (Colfax NE2701) susceptible (Williams 82 Senaki) lines at four time points (2, 4, 8, 16 h post inoculation [hpi]) after P. inoculation. Comparative analyses revealed greater number differentially expressed genes (DEGs) upon pathogen lines, particularly 8 hpi. These DEGs were predominantly associated with defense response, ethylene, reactive oxygen species-mediated pathways. Moreover, DE transposons upregulated inoculation, more them enriched near Colfax than other lines. Notably, identified long non-coding RNA (lncRNA) within mapped region gene that exhibited exclusive upregulation potentially regulating flanking LURP-one-related genes. Furthermore, DNA methylation analysis increased CHH (where H = A, T, or C) levels lncRNAs delayed compared Williams 82. Overall, our results provide comprehensive insights into sansomeana, highlighting potential roles regulation plant defense.

Язык: Английский

Процитировано

2
Update on functional analysis of long non-coding RNAs in common crops DOI Creative Commons
Aijing Zhang,

Wenxuan Pi,

Yashuo Wang

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Май 30, 2024

With the rapid advances in next-generation sequencing technology, numerous non-protein-coding transcripts have been identified, including long noncoding RNAs (lncRNAs), which are functional comprising more than 200 nucleotides. Although lncRNA-mediated regulatory processes extensively investigated animals, there has considerably less research on plant lncRNAs. Nevertheless, multiple studies major crops showed lncRNAs involved crucial processes, growth and development, reproduction, stress responses. This review summarizes progress lncRNA roles several crops, presents key strategies for exploring discusses current challenges future prospects. The insights provided this will enhance our comprehension of functions with potential implications improving crop genetics breeding.

Язык: Английский

Процитировано

1
A leucine-rich repeat receptor-like protein kinase enhances tomato resistance to Phelipanche aegyptiaca DOI
Lu Zhang, Zhaoqun Yao, Xiaolei Cao

и другие.

Scientia Horticulturae, Год журнала: 2024, Номер 337, С. 113353 - 113353

Опубликована: Авг. 14, 2024

Язык: Английский

Процитировано

1
Transcriptomic and epigenetic responses shed light on soybean resistance toPhytophthora sansomeana DOI Creative Commons
Gwonjin Lee, Charlotte N. DiBiase, Beibei Liu

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 30, 2024

Abstract Phytophthora root rot caused by oomycete pathogens in the genus poses a significant threat to soybean productivity. While resistance mechanisms against sojae have been extensively studied soybean, molecular basis underlying immune responses sansomeana remains unclear. We investigated transcriptomic and epigenetic of two resistant (Colfax NE2701) susceptible (Williams 82 Senaki) lines at four time points (2, 4, 8, 16 hours post inoculation, hpi) after P. inoculation. Comparative analyses revealed greater number differentially expressed genes (DEGs) upon pathogen inoculation lines, particularly 8 hpi, predominantly associated with ethylene reactive oxygen species-mediated defense pathways. Moreover, DE transposons were up-regulated enriched near Colfax. A long non-coding RNA (lncRNA) within mapped region gene exhibited exclusive up-regulation potentially regulating flanking LURP-one-related genes. Furthermore, DNA methylation analysis increased CHH levels lncRNAs delayed Colfax compared Williams 82. Overall, our results provide comprehensive insights into , highlighting potential roles regulation plant defense.

Язык: Английский

Процитировано

0