bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 20, 2024
ABSTRACT
Cancer
models
in
animal
studies
play
a
central
role
cancer
research,
particularly
investigating
vascularized
tumor
tissues
for
the
validation
of
immune
cell
therapies.
However,
xenografts
relying
solely
on
cells
are
ineffective
optimal
tissue
formation.
Additionally,
modeling
using
hydrogels
with
to
promote
vascularization
often
leaves
behind
residual
biomaterials
that
inhibit
integration
surrounding
tissues.
To
address
these
issues,
we
utilized
straightforward
vivo
method
completely
degradable,
crosslinker-free
carboxymethyl
chitosan
(CMCTS)/oxidized
hyaluronic
acid
(OHA)
hydrogel
encapsulates
high-density
human
situ
injection.
The
CMCTS/oHA
was
fully
degraded
within
3
weeks,
enabling
three-dimensional
(3D)
condensation
vitro
.
2
weeks
after
subcutaneous
injection
mice,
solid
tumors
formed,
native
host
vasculature
infiltrating
transplanted
cells,
confirming
spontaneous
degradation.
Following
this,
macrophages
were
administered
via
tail
vein
injection,
enhancing
accumulation
mouse
humanized
twofold
and
showing
murine
adjacent
vasculature.
This
study
thus
provides
proof-of-concept
facile
model
mice
validating
HIGHLIGHTS
oHA
prepared
sodium
periodate
treatment,
which
facilitated
formation
short
period,
allowing
3D
High-density
cell-laden
injected
subcutaneously
resulting
generation
therapeutic
observed
adhere
through
bloodstream
Materials Today Bio,
Год журнала:
2025,
Номер
31, С. 101451 - 101451
Опубликована: Янв. 5, 2025
Recently,
injectable
hydrogels
have
garnered
significant
attention
in
tissue
engineering
due
to
their
controlled
flowability,
strong
plasticity,
adaptability,
and
good
biocompatibility.
However,
research
on
readily
situ-forming
capable
of
forming
functional
three-dimensional
(3D)
condensations
remains
limited.
This
study
explores
the
development
evaluation
a
carboxymethyl
chitosan
(CMCTS)/oxidized
hyaluronic
acid
(oHA)
hydrogel
incorporated
with
silver
sulfadiazine
(AgSD)
for
applications
inherent
antibacterial
activity.
Through
physicochemical
analysis,
optimal
formulation
CMCTS/oHA
was
established.
The
demonstrated
excellent
injectability,
enabling
minimally
invasive
situ
delivery.
In
vitro
cytotoxicity
assays
identified
0.1
%
AgSD
as
concentration,
supporting
cell
proliferation
while
exhibiting
antimicrobial
efficacy
against
S.
mutans
E.
faecalis.
vivo
studies
revealed
complete
degradation
biocompatibility,
no
adverse
reactions.
hydrogel's
ability
form
3D
aggregates
support
regeneration
underscores
its
potential
future
applications.
Consequently,
CMCTS/oHA/AgSD
developed
this
holds
application
wide
range
bioengineering
fields,
including
substance
delivery
systems
engineering,
indicating
clinical
application.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
ABSTRACT
Recently,
injectable
hydrogels
have
garnered
significant
attention
in
tissue
engineering
due
to
their
controlled
flowability,
strong
plasticity,
adaptability,
and
good
biocompatibility.
However,
research
on
readily
situ
-forming
capable
of
forming
functional
three-dimensional
(3D)
condensations
remains
limited.
This
study
explores
the
development
evaluation
a
carboxymethyl
chitosan
(CMCTS)
/
oxidized
hyaluronic
acid
(oHA)
hydrogel
incorporated
with
silver
sulfadiazine
(AgSD)
for
applications
inherent
antibacterial
activity.
Through
physicochemical
analysis,
optimal
formulation
CMCTS/oHA
was
established.
The
demonstrated
excellent
injectability,
enabling
minimally
invasive
delivery.
In
vitro
cytotoxicity
assays
identified
0.1%
AgSD
as
concentration,
supporting
cell
proliferation
while
exhibiting
antimicrobial
efficacy
against
S.
mutans
E.
faecalis
.
vivo
studies
revealed
complete
degradation
biocompatibility,
no
adverse
reactions.
hydrogel’s
ability
form
3D
aggregates
support
regeneration
underscores
its
potential
future
applications.
Consequently,
CMCTS/oHA/AgSD
developed
this
holds
application
wide
range
bioengineering
fields,
including
substance
delivery
systems
engineering,
indicating
clinical
application.
International Journal of Nanomedicine,
Год журнала:
2025,
Номер
Volume 20, С. 4571 - 4587
Опубликована: Апрель 1, 2025
Topical
application
of
17β-estradiol
(E2)
has
been
shown
to
improve
various
hallmark
features
skin
aging,
including
enhancing
elasticity
and
hydration,
reducing
wrinkles,
promoting
collagen
synthesis.
However,
the
role
estrogen
in
UVB-induced
photoaging
remains
unclear.
Furthermore,
E2's
clinical
is
limited
by
issues
such
as
bioavailability
potential
adverse
effects.
Therefore,
this
study
aims
explore
E2
prepare
a
gold
(Au)@mesoporous
polydopamine
(mPDA)-hyaluronic
acid
(HA)/carboxymethyl
chitosan
(CMCS)
nanoparticle
composite
hydrogel
(Au/E2@mPDA-HCG)
for
treatment
photoaging.
This
successfully
fabricated
mPDA
with
well-defined
mesoporous
structure
incorporated
Au
NPs
into
mesopores
using
an
situ
growth
method,
thereby
constructing
Au@mPDA
loaded
E2.
Subsequently,
Au/E2@mPDA
were
embedded
HA/CMCS
develop
Au/E2@mPDA-HCG
hydrogel.
The
was
characterized
through
vitro
vivo
experiments,
its
efficacy
improving
evaluated.
revealed
that
deficiency
significantly
exacerbates
photoaging,
likely
mechanisms
closely
associated
increased
oxidative
stress
reduced
production.
Moreover,
demonstrated
favorable
morphological
characteristics
biocompatibility.
In
experimental
results
indicated
effectively
enhanced
therapeutic
treating
evidenced
significant
mitigation
inflammatory
responses,
along
promotion
conclusion,
suggests
combination
Au@mPDA@HCG
nanocomposite
offers
promising
strategy
