Direct and pleiotropic effects of antimicrobial peptide transgene integration on reproductive, growth regulating, and non-coding loci in channel catfish (Ictalurus punctatus)

Agriculture Communications, Год журнала: 2024, Номер 2(2), С. 100044 - 100044

Опубликована: Июнь 1, 2024

Antimicrobial peptides (AMPs) show promise in enhancing resistance against pathogens. Previously, we integrated two AMP genes, cathelicidin (Cath) from alligator (Alligator mississippiensis or A. sinensis) and cecropin (Cec) moth (Hyalophora cecropia), into the channel catfish (Ictalurus punctatus) genome. This study examines efficacy of exogenous gene integration improving bacterial transgenic assesses direct pleiotropic effects replacement/knockout on survival growth based insertion site. Transgenic Cath- Cec-expressing fish exhibited similar higher rates (P > 0.05) compared to controls during initial culture. Integration Cec transgene doubled rate when challenged with Edwardsiella ictaluri, knock-in (KI) Cath further increasing resistance. Coupling KI mstn knockout (KO) increased 3-fold after E. ictaluri infection by 50% at 4 months post-fertilization (mpf). However, random had a minimal effect disease did not enhance growth. Random 2.5-fold 4-fold Flavobacterium covae, respectively, without affecting lh locus F. covae reduced 10% 24 mpf, whereas coupled mc4r KO resulted increase following controls, 80% 3 mpf. Simultaneous Cec, along mstn, while Dual genes yielded greatest disease. These may comprehension societal acceptance genetic engineering aquaculture.

Язык: Английский

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CRISPR/Cas13d mediated inhibition of Siniperca chuatsi rhabdovirus infection in fish cells

Aquaculture, Год журнала: 2025, Номер unknown, С. 742355 - 742355

Опубликована: Фев. 1, 2025

Язык: Английский

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Increasing the efficiency of gene editing with CRISPR-Cas9 via concurrent expression of the Beta protein

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 270, С. 132431 - 132431

Опубликована: Май 15, 2024

Язык: Английский

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Genome Manipulation Advances in Selected Aquaculture Organisms

Reviews in Aquaculture, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 15, 2024

ABSTRACT With the rising global demand for seafood and challenges posed by overfishing climate change, aquaculture sector has become increasingly important in providing high‐quality protein human consumption. Although traditional selection breeding programs have made great strides genetic improvement of species over past decades, faster more precise tools, such as genome manipulation, are needed performance enhancement stock. This review presents a comprehensive overview current status three major manipulation including RNA interference (RNAi), gene transfer, editing species, discusses advances made, faced, potential future directions this fast‐developing field. Taking catfish an example, paper reviews specific applications these techniques to improve traits growth, disease resistance, reproduction, nutritional profiles various commercially fishes crustaceans, highlighting successful ongoing research efforts. We also propose CRISPR/Cas9‐mediated multiplex knockout or replacement multiple genes parallel fish. Collectively, provides insights into evolving landscape sheds light on its implications sustainable practices responsible innovation.

Язык: Английский

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Effects of centrifugation treatment before electroporation on gene editing in pig embryos

In Vitro Cellular & Developmental Biology - Animal, Год журнала: 2024, Номер 60(7), С. 732 - 739

Опубликована: Июнь 4, 2024

Язык: Английский

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