Biomedicines,
Год журнала:
2024,
Номер
12(9), С. 2028 - 2028
Опубликована: Сен. 5, 2024
Skin
bioprinting
has
the
potential
to
revolutionize
treatment
approaches
for
injuries
and
surgical
procedures,
while
also
providing
a
valuable
platform
assessing
screening
cosmetic
pharmaceutical
products.
This
technology
offers
key
advantages,
including
flexibility
reproducibility,
which
enable
creation
of
complex,
multilayered
scaffolds
that
closely
mimic
intricate
microenvironment
native
skin
tissue.
The
development
an
ideal
hydrogel
is
critical
successful
these
with
incorporated
cells.
In
this
study,
we
used
formulation
developed
in
our
laboratory
fabricate
3D-bioprinted
model.
composition
was
carefully
selected
based
on
its
high
compatibility
human
cells,
incorporating
alginate,
methyl
cellulose,
nanofibrillated
cellulose.
One
challenges
process,
particularly
commercialization
large-scale
production,
ensuring
consistency
minimal
batch-to-batch
variations.
To
address
this,
explored
methods
preserve
physicochemical
properties
hydrogels,
focus
freezing
techniques.
We
validated
pre-frozen
hydrogels'
printability,
rheology,
mechanical
surface
properties.
Our
results
revealed
extended
times
significantly
reduced
viscosity
formulations
due
ice
crystal
formation,
leading
redistribution
polymer
chains.
reduction
resulted
more
challenging
extrusion
increased
macro-
microporosity
as
confirmed
by
nanoCT
imaging.
porosity
led
greater
water
uptake,
swelling,
compromised
scaffold
integrity,
altered
degradation
kinetics.
insights
gained
from
study
lay
solid
foundation
advancing
vitro
model
promising
applications
preclinical
clinical
research.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 5, 2024
Abstract
Injectable
porous
microspheres
represent
a
promising
therapeutic
platform
for
cell
delivery,
drug
and
tissue
regeneration.
Yet,
the
engineering
of
silk
fibroin
with
highly
interconnected
structure
remains
an
unsolved
challenge.
In
this
study,
simple
efficient
method
is
developed
that
does
not
require
use
organic
solvents
to
prepare
predictable
structure.
Through
extensive
screening,
addition
glucose
found
direct
formation
from
interior
surface
microspheres.
Compared
(SF
microspheres)
produced
through
combination
electro‐spray,
cryopreservation,
freeze
drying,
fibroin‐glucose
(SF‐Glu
demonstrates
enhanced
capabilities
in
promoting
adhesion
proliferation
vitro.
Both
SF‐Glu
SF
exhibit
capacity
maintain
sustained
release
kinetics
loaded
model
drug.
Furthermore,
facilitate
recruitment
endogenous
cells,
capillary
migration,
macrophage
phenotype
switch
following
subcutaneous
injection
rats.
This
study
opens
new
avenue
construction
microspheres,
which
could
lead
broader
range
applications
regenerative
medicine.
