Structural characteristics of neutral polysaccharides purified from coix seed and its anti‐insulin resistance effects on HepG2 cells DOI Creative Commons

Guozhen Wu,

Shuang Liu,

Zhenqiang Wang

и другие.

Food Science & Nutrition, Год журнала: 2024, Номер 12(10), С. 8419 - 8431

Опубликована: Сен. 8, 2024

Abstract Coix seed is recognized as a functional medicinal food due to its valuable biological activities, with polysaccharides being the primary active compounds. In this study, an ultrasonic‐assisted enzymatic extraction technique was employed, and response surface methodology used optimize yield of 9.55 ± 0.26%. A novel neutral polysaccharide, CSPsN‐1, purified molecular weight 7.75 kDa. CSPsN‐1 composed arabinose, galactose, glucose, xylose, mannose in molar ratios 0.48: 7.92: 86.39: 2.42: 2.79. Its backbone →4)‐α‐D‐Glc p ‐(1→ →3,4)‐α‐D‐Glc units, terminal residues α‐D‐Glc . vitro experiments, enhanced glucose consumption insulin‐resistant HepG2 cells upregulated GLUT4 expression by activating PI3K/AKT signaling pathway. These findings suggest that holds significant promise ingredient for treating insulin resistance related metabolic disorders.

Язык: Английский

Exploring the Bioactive Potential of Mushroom Aqueous Extracts: Antimicrobial, Antioxidant, and Prebiotic Properties DOI Creative Commons
Tiago Barros Afonso, Sara Marçal, P. A. C. B. Vale

и другие.

Applied Sciences, Год журнала: 2025, Номер 15(3), С. 1551 - 1551

Опубликована: Фев. 3, 2025

Mushrooms provide essential nutrients and bioactive compounds, namely glucans, protein, phenolic compounds. In this study, two aqueous extractions were performed (M1 M2) using Pleurotus ostreatus, eryngii, Agrocybe cylindracea. M1, a hot extraction (extract M1) (90 °C, 700 rpm, 1 h) was performed. M2, room-temperature M2A) followed by M2B) of the extract M2A residue The M2B extracts showed lowest yields (12.58–21.78%), while other ranged between 30.91% 46.03%. All had high protein (12.09–32.97 g/100 g dry extract), glucan (12.69–48.57 contents (7.90–16.65 mg GAEs/g extract) antioxidant (ABTS ORAC assays), antimicrobial, antibiofilm, prebiotic activities. So, they have potential to be used as functional ingredients or natural preservatives. Extracts from A. cylindracea stood out since higher content, activity, activity inhibited number foodborne bacteria (only M2A). However, unlike P. ostreatus at 40 mg/mL, cytotoxic effects.

Язык: Английский

Процитировано

0

Changes of the Physicochemical Properties and Structural Characteristics of Alkali-Extracted Polysaccharides from Agrocybe cylindracea Across the Growth Process DOI

Jin-Jin Liu,

Shi‐Kang Chen,

Xin Wang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(22), С. 12810 - 12821

Опубликована: Май 22, 2024

Polysaccharides derived from Agrocybe cylindracea have been demonstrated to exhibit various bioactivities. However, studies on their structural characteristics during the growth process are limited. This study aimed compare physicochemical properties and of alkali-extracted polysaccharides A. fruiting bodies (JACP) across four stages. Results showed that extraction yields protein levels JACP declined along with cylindracea, while contents neutral sugar glucose increased significantly. exhibited similar those Four polysaccharide subfractions were isolated each stage, including JACP-Et30, JACP-Et50, JACP-Et60, JACP-Et70. JACP-Et30 stages JACP-Et50 initial three identified as heteroglucans β-1,3-d-Glcp β-1,6-d-Glcp residues main chains, respectively. other considered β-1,6-d-glucans containing minor glucuronic acid. These predominantly replaced by Glcp at O-3 O-6 positions. Overall, variable properties, its remained stable process, offering new insights into potential applications in food medicinal industries.

Язык: Английский

Процитировано

1

Insights into dynamic evolution of glucuronofucogalactoglucan from water extract of Agrocybe cylindracea during maturation DOI

Jin-Jin Liu,

Shi‐Kang Chen,

Hui Luo

и другие.

Carbohydrate Polymers, Год журнала: 2024, Номер 339, С. 122235 - 122235

Опубликована: Май 9, 2024

Язык: Английский

Процитировано

0

Structural characteristics of neutral polysaccharides purified from coix seed and its anti‐insulin resistance effects on HepG2 cells DOI Creative Commons

Guozhen Wu,

Shuang Liu,

Zhenqiang Wang

и другие.

Food Science & Nutrition, Год журнала: 2024, Номер 12(10), С. 8419 - 8431

Опубликована: Сен. 8, 2024

Abstract Coix seed is recognized as a functional medicinal food due to its valuable biological activities, with polysaccharides being the primary active compounds. In this study, an ultrasonic‐assisted enzymatic extraction technique was employed, and response surface methodology used optimize yield of 9.55 ± 0.26%. A novel neutral polysaccharide, CSPsN‐1, purified molecular weight 7.75 kDa. CSPsN‐1 composed arabinose, galactose, glucose, xylose, mannose in molar ratios 0.48: 7.92: 86.39: 2.42: 2.79. Its backbone →4)‐α‐D‐Glc p ‐(1→ →3,4)‐α‐D‐Glc units, terminal residues α‐D‐Glc . vitro experiments, enhanced glucose consumption insulin‐resistant HepG2 cells upregulated GLUT4 expression by activating PI3K/AKT signaling pathway. These findings suggest that holds significant promise ingredient for treating insulin resistance related metabolic disorders.

Язык: Английский

Процитировано

0