Analysis of foreign body response and systemic toxicity of additively manufactured nanocellulose reinforced alginate gelatin-based scaffolds with interconnected 3D porous structure

Journal of Biomaterials Applications, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

The last two decaes have witnessed significant efforts to develop gelatin/alginate based scaffolds using variants of 3D printing techniques. However, their biocompatibility for regenerating complex soft tissues remains insufficiently explored. Addressing this gap, we fabricated 3D-printed alginate-gelatin (3A5G) and nanocellulose-reinforced (3A5G1C) hydrogel with clinically relevant dimensions (15 mm diameter, 5 height) the host tissue responses were critically analyzed. distinct advantages nanocellulose in modulating mechanical strength, viscoelasticity, swelling, degradation characteristics established our prior studies. This investigation aimed comprehensively evaluate foreign body response these a rat model. animals exhibited healthy metabolic activity, evidenced by progressive weight gain, localized healing, normal mobility over 30 days. Histological analyses could not reveal any adverse immune reaction at 7- or 30-days, post-implantation. Hematological serum biochemical assessments indicated progression from acute (7 days) sub-acute (30 inflammation, following subcutaneous implantation, without signature systemic toxicity. Immune marker evaluation (TNF-α, CD-8, CD-68, COX-2, IL-6) confirmed absence pathological responses, even incorporation. Immunohistochemical analysis CD31 staining demonstrated enhanced vascularization both 7 toxicity scaffold products favorable outcomes underline potential regeneration. incorporation further scaffolds' functional performance, particularly promoting vascularization, positioning them as promising candidates engineering applications.

Язык: Английский

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3D Printing and Electrospinning of Drug- and Graphene-Enhanced Polycaprolactone Scaffolds for Osteochondral Nasal Repair

Materials, Год журнала: 2025, Номер 18(8), С. 1826 - 1826

Опубликована: Апрель 16, 2025

A novel bi-layered scaffold, obtained via 3D printing and electrospinning, was designed to improve osteochondral region reconstruction. The upper electrospun membrane will act as a barrier against unwanted tissue infiltration, while the lower 3D-printed layer provide porous structure for ingrowth. Graphene integrated into scaffold its antibacterial properties, drug Osteogenon® (OST) added promote bone regeneration. composite scaffolds were subjected comprehensive physical, thermal, mechanical evaluations. Additionally, their biological functionality assessed by means of NHAC-kn cells. 0.5% graphene addition PCL significantly increased strain at break, enhancing material ductility. GNP also acted an effective nucleating agent, raising crystallization temperatures supporting mineralization. high surface area facilitated rapid apatite formation attracting calcium phosphate ions. This confirmed FTIR, µCT SEM analyses, which highlighted positive impact on mineral deposition. synergistic interaction between nanoplatelets created bioactive environment that enhanced cell adhesion proliferation, promoted superior formation. These findings highlight scaffold’s potential promising biomaterial repair regenerative medicine.

Язык: Английский

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Scaffold for Tissue Engineering Design Fabrication and Applications

IGI Global eBooks, Год журнала: 2025, Номер unknown, С. 335 - 366

Опубликована: Апрель 25, 2025

Recently, Tissue engineering (TE) has been rapidly growing field in biomedical field, it provides the solutions to problem such as immunological rejection and a shortage of available donors. TE scaffolds encourage cell adhesion, proliferation, differentiation by simulating extracellular matrix with three-dimensional matrices. Current, advancements artificial intelligence (AI) nanotechnology have improved design, pore size, mechanical properties. However, lack ability vascularization, immune compatibility, scalability. In order address this issue, surface-functionalized anti-inflammatory cytokines IL-10 that can increase integration, less rejection, long-term performance. Therefore, is considered combination AI scaffold design will enhance bioactivity, strength, cellular behavior modeling. Regenerative medicine poised for revolution due significant scaffold-based TE.

Язык: Английский

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State-of-the-art in natural hydrogel-based wound dressings: Design, functionalization, and fabrication approaches

Advances in Colloid and Interface Science, Год журнала: 2025, Номер unknown, С. 103527 - 103527

Опубликована: Апрель 1, 2025

Язык: Английский

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Advancements and Applications of 4D Bioprinting in Biomedical Science

Chinese Journal of Polymer Science, Год журнала: 2024, Номер unknown

Опубликована: Дек. 25, 2024

Язык: Английский

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Impact of Cell Geometry on the Mechanical and Failure Characteristics of 3d Voronoi Hap Through Sla Technique

Опубликована: Янв. 1, 2024

Язык: Английский

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Human Septal Cartilage Tissue Engineering: Current Methodologies and Future Directions

Bioengineering, Год журнала: 2024, Номер 11(11), С. 1123 - 1123

Опубликована: Ноя. 7, 2024

Nasal septal cartilage tissue engineering is a promising and dynamic field with the potential to provide surgical options for patients complex reconstruction needs mitigate risks incurred by other sources. Developments in cell source selection, expansion, scaffold creation, three-dimensional (3D) bioprinting have advanced recent years. The usage of medicinal signaling cells nasal chondroprogenitor can enhance chondrocyte proliferation, stimulate growth, limit dedifferentiate. New scaffolds combined innovations 3D allowed creation more durable customizable constructs. Future developments may increase technical accessibility manufacturability, lower costs, help incorporate these methods into pre-clinical studies clinical applications engineering.

Язык: Английский

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Functional polysaccharide-based hydrogel in bone regeneration: From fundamentals to advanced applications

Carbohydrate Polymers, Год журнала: 2024, Номер 352, С. 123138 - 123138

Опубликована: Дек. 13, 2024

Язык: Английский

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