Co-expression of endoglucanase and cellobiohydrolase from yak rumen in lactic acid bacteria and its preliminary application in whole-plant corn silage fermentation DOI Creative Commons

Xuerui Wan,

Yongjie Sunkang, Yijun Chen

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Сен. 17, 2024

Endoglucanase (EG) and cellobiohydrolase (CBH) which produced by microorganisms, have been widely used in industrial applications. In order to construct recombinant bacteria that produce high activity EG CBH, this study, eg (endoglucanase) cbh (cellobiohydrolase) were cloned from the rumen microbial genome of yak subsequently expressed independently co-expressed within Lactococcus lactis NZ9000 (L. NZ9000). The strains L. NZ9000/pMG36e-usp45-cbh lactis-cbh), NZ9000/pMG36e-usp45-eg lactis-eg), NZ9000/pMG36e-usp45-eg-usp45-cbh lactis-eg-cbh) successfully constructed demonstrated ability secrete EG, EG-CBH. sodium carboxymethyl cellulose enzyme was highest, regenerated amorphous (RAC) specific substrate optimum reaction temperature CBH 60°C, while enzymes EG-CBH tolerant higher temperatures (80°C). pH 6.0. Mn2+, Fe2+, Cu2+, Co2+ could promote CBH. Similarly, Ba2+, concentrations Ca2+, addition engineered whole-plant corn silage improved nutritional quality feed, with lowest pH, acid detergent fiber (ADF), neutral (NDF) contents observed lactis-eg group (p < 0.05), ammonia nitrogen (NH3-N), highest lactic (LA) crude protein (CP) + lactis-cbh not satisfactory. Consequently, lactis-cbh, lactis-eg, lactis-eg-cbh constructed, promoted fermentation degrading sugar, enabling secretory expression for potential applications degradation.

Язык: Английский

Covalent immobilization of thermotolerant recombinant nano-coupled xylanase for improved stability and reusability in the saccharification process DOI
Shahid Aslam,

Rukhma,

Iram Liaqat

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142169 - 142169

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Latest advances in research methods for high-yield cellulase production DOI
Xinxin Xiang, Youhong Zhang, Wei Wei

и другие.

Sustainable Energy & Fuels, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Lignocellulosic biomass, due to its accessibility, abundance, and environmental friendliness, has become a promising renewable resource.

Язык: Английский

Процитировано

0
Co-expression of endoglucanase and cellobiohydrolase from yak rumen in lactic acid bacteria and its preliminary application in whole-plant corn silage fermentation DOI Creative Commons

Xuerui Wan,

Yongjie Sunkang, Yijun Chen

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Сен. 17, 2024

Endoglucanase (EG) and cellobiohydrolase (CBH) which produced by microorganisms, have been widely used in industrial applications. In order to construct recombinant bacteria that produce high activity EG CBH, this study, eg (endoglucanase) cbh (cellobiohydrolase) were cloned from the rumen microbial genome of yak subsequently expressed independently co-expressed within Lactococcus lactis NZ9000 (L. NZ9000). The strains L. NZ9000/pMG36e-usp45-cbh lactis-cbh), NZ9000/pMG36e-usp45-eg lactis-eg), NZ9000/pMG36e-usp45-eg-usp45-cbh lactis-eg-cbh) successfully constructed demonstrated ability secrete EG, EG-CBH. sodium carboxymethyl cellulose enzyme was highest, regenerated amorphous (RAC) specific substrate optimum reaction temperature CBH 60°C, while enzymes EG-CBH tolerant higher temperatures (80°C). pH 6.0. Mn2+, Fe2+, Cu2+, Co2+ could promote CBH. Similarly, Ba2+, concentrations Ca2+, addition engineered whole-plant corn silage improved nutritional quality feed, with lowest pH, acid detergent fiber (ADF), neutral (NDF) contents observed lactis-eg group (p < 0.05), ammonia nitrogen (NH3-N), highest lactic (LA) crude protein (CP) + lactis-cbh not satisfactory. Consequently, lactis-cbh, lactis-eg, lactis-eg-cbh constructed, promoted fermentation degrading sugar, enabling secretory expression for potential applications degradation.

Язык: Английский

Процитировано

1