Comparative transcriptomics ofLathyrus sativusreveals accession-specific resistance responses againstErysiphe pisi DOI Creative Commons
Ricardo Marques, Telma Fernandes, Pedro M. Barros

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 25, 2024

Lathyrus sativus (grass pea) is a valuable crop for sustainable agriculture, offering both dietary benefits and desirable agronomic traits. However, its yield stability limited by different diseases such as powdery mildew caused Erysiphe pisi . Frequent fungal resistance to pesticides growing environmental concerns highlight the need research investment develop resistant varieties. Four L. accessions, exhibiting varying levels of E. (resistant, partially resistant, susceptible, susceptible), were analysed using dual RNA-seq identify key defence mechanisms effector genes involved in this plant-pathogen interaction. The transcriptomic analysis highlighted host biphasic response, characterised an initial burst gene expression, followed quiescent phase, second wave intense expression at 72 hours after inoculation. Common mechanisms, including antifungal protein cell wall reinforcement, reactive oxygen species-mediated activated all accessions compared susceptible accession. Unique responses accession integrate early reinforcement structural barriers with sustained chemical defences stress responses. Overall, exhibited front-loaded focused on biotic stimuli interspecies interactions infection stages. In contrast, partial weaker baseline system, slower less robust response specifically targeting pathogen infection. We identified potential effectors, hydrolysis, nutrient acquisition, virulence, higher diversity effectors This study identifies novel targets within complex sativus-Erysiphe interaction that will support future breeding programs aimed enhancing other related species.

Язык: Английский

Effects of Different Cutting Styles on Physiological Properties in Fresh-Cut Carrots DOI Creative Commons
Ning Zhou, Sen Ma, Minwei Zhang

и другие.

Plants, Год журнала: 2024, Номер 13(12), С. 1665 - 1665

Опубликована: Июнь 16, 2024

With the internationalization of Chinese culture, ready-to-cook food has become popular. Vegetables in preparations are usually cut into slices, cubes, and shreds. Carrots, as a typical side dish, were selected model this work. The polyphenol content, antioxidant capacity, O2−, hydrogen peroxide, malondialdehyde, lignin, enzymes, other enzymes activities analyzed. results indicated that these parameters insignificantly different between three cutting styles. Therefore, metabolomics is further employed. Pathway enrichment glyceollin II 6″-malonylgenistin metabolites particularly expressed isoflavonoid biosynthesis pathway; (+)-gallocatechin, trans-chlorogenic acid, (−)-epiafzelechin specifically identified flavonoid pathway after slicing; shredding caused expression coniferyl aldehyde eugenol, which phenylpropanoid pathway. These indicate styles do not change physiological indicators carrots but induce specific metabolites.

Язык: Английский

Процитировано

1
Lignin lite: Boosting plant power through selective downregulation DOI Open Access
Narasimham Dokka,

Maniraj Rathinam,

Rohini Sreevathsa

и другие.

Plant Cell & Environment, Год журнала: 2024, Номер 47(12), С. 4945 - 4962

Опубликована: Авг. 8, 2024

SUMMARY STATEMENT This article explores the dual benefits of reducing lignin content in plants, which streamlines biofuel production while maintaining robust defence mechanisms. It discusses how plants compensate for lower levels through alternative strategies, recent biotechnological advances modification, and implications agriculture industry.

Язык: Английский

Процитировано

1
First record of blue ring in a dicotyledonous angiosperm wood DOI
Jiří Doležal, Francesca Secchi,

Niccolò Tricerri

и другие.

Trees, Год журнала: 2024, Номер 39(1)

Опубликована: Дек. 18, 2024

Язык: Английский

Процитировано

1
Exploring Lignin Biosynthesis Genes in Rice: Evolution, Function, and Expression DOI Open Access
Munsif Ali Shad, Xukai Li, Muhammad Junaid Rao

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 10001 - 10001

Опубликована: Сен. 17, 2024

Lignin is nature’s second most abundant vascular plant biopolymer, playing significant roles in mechanical support, water transport, and stress responses. This study identified 90 lignin biosynthesis genes rice based on phylogeny motif constitution, they belong to PAL, C4H, 4CL, HCT, C3H, CCoAOMT, CCR, F5H, COMT, CAD families. Duplication events contributed largely the expansion of these gene families, such as mainly attributed tandem segmental duplication. Microarray data 33 tissue samples covering entire life cycle suggested fairly high expressions rather variable F5H expressions. Some members lignin-related (OsCCRL11, OsHCT1/2/5, OsCCoAOMT1/3/5, OsCOMT, OsC3H, OsCAD2, OsPAL1/6) were expressed all tissues examined. The expression patterns can be divided into two major groups with eight subgroups, each showing a distinct co-expression representing typically primary secondary cell wall constitutions. strongly co-expressed typical walls. Combined HPLC analysis showed increased monomer (H, G, S) contents from young old growth stages five genotypes. Based genes’ microarray data, 27 selected for qRT-PCR analysis. Four (OsPAL9, OsCAD8C, OsCCR8, OsCOMTL4) significantly negatively correlated monomers. Furthermore, eleven certain genotypes during stages. Among them, six (OsC3H, OsCCR2, OsPAL2, OsPAL8) overlapped expressions, highlighting their importance biosynthesis.

Язык: Английский

Процитировано

0
Metal oxide nanoparticles as a promising method to reduce biotic stress in plant cell wall: A review DOI Creative Commons
Yalew Yiblet,

Miseganaw Sisay

Heliyon, Год журнала: 2024, Номер 10(19), С. e37939 - e37939

Опубликована: Сен. 20, 2024

Язык: Английский

Процитировано

0
Preservation of minimally processed carrots using the combination of ultrasound and mild heat ascorbic acid DOI Creative Commons
Jiayi Wang, N. Zhou,

Sen Ma

и другие.

Ultrasonics Sonochemistry, Год журнала: 2024, Номер 111, С. 107086 - 107086

Опубликована: Сен. 27, 2024

Ultrasound (US) in combination with chemical disinfectants is an efficient and cost-effective hurdle technology for disinfecting minimally processed produce (MPP). However, the demand non-chemical disinfection methods increasing. In addition, have been ineffective simultaneously improving physiological properties inactivating pathogens MPP. this study, a novel safe method called mild heat ascorbic acid (MHAsA; 1 % AsA at 50 °C) was combined US to process carrots. Physiological microbial inactivation efficacy were analyzed during storage period (0-5 days). The findings indicated that US-MHAsA induced highest levels of antioxidant enzymes (superoxide dismutase catalase) activities accelerated glutathione-ascorbate cycle, resulting lower reactive oxygen species (ROS) malondialdehyde content compared MHAsA. phenylalanine lyase, initial enzyme lignin synthesis process, than US. On other hand, its ability inactivate cinnamyl alcohol dehydrogenase, final better both there no significant differences among three groups. against (polyphenol oxidase peroxidase) involved browning consistent across treatments. Analysis Escherichia coli O157:H7 Salmonella Typhimurium revealed achieved lowest cross-contamination incidence (10-12 %) washing, which significantly by (75-82 %). During from day 0 5, two on carrots control group increased 6.25 6.64 log CFU/g, while counts observed group, decreasing 4.44 3.74 CFU/g. 5.22 6.32 5 not control. These indicate effectively reduces risk pathogen contamination enhances MPP scavenge ROS.

Язык: Английский

Процитировано

0
Influence of pH on the Formation of Benzyl Ester Bonds Between Dehydrogenation Polymers and Konjac Glucomannan DOI Creative Commons
Peng Wang, Xu Zhang,

Xi Le

и другие.

Molecules, Год журнала: 2024, Номер 29(21), С. 5166 - 5166

Опубликована: Окт. 31, 2024

A thorough understanding of the lignin–carbohydrate complex (LCC) structure has a significant meaning in high-value utilization lignocellulose. In this work, (DHPKGC) was obtained by an addition reaction between konjac glucomannan (KGM) and quinone methides generated synthesis dehydrogenation polymers (DHPs) to simulate formation LCCs. The effect pH on prepared DHPKGC investigated. characterized Fourier Transform Infrared (FTIR), 13C-Nuclear Magnetic Resonance (13C-NMR), two-dimensional Heteronuclear Single Quantum Coherence Nuclear (2D HSQC NMR) analyses. results indicated 4.0 conducive polymerization DHPs oxidized KGM TEMPO/NaClO/NaBr system. addition, resultant connected benzyl ester linkages. Overall, study aims gain greater insight into process LCC plants.

Язык: Английский

Процитировано

0
Comparative transcriptomics ofLathyrus sativusreveals accession-specific resistance responses againstErysiphe pisi DOI Creative Commons
Ricardo Marques, Telma Fernandes, Pedro M. Barros

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 25, 2024

Lathyrus sativus (grass pea) is a valuable crop for sustainable agriculture, offering both dietary benefits and desirable agronomic traits. However, its yield stability limited by different diseases such as powdery mildew caused Erysiphe pisi . Frequent fungal resistance to pesticides growing environmental concerns highlight the need research investment develop resistant varieties. Four L. accessions, exhibiting varying levels of E. (resistant, partially resistant, susceptible, susceptible), were analysed using dual RNA-seq identify key defence mechanisms effector genes involved in this plant-pathogen interaction. The transcriptomic analysis highlighted host biphasic response, characterised an initial burst gene expression, followed quiescent phase, second wave intense expression at 72 hours after inoculation. Common mechanisms, including antifungal protein cell wall reinforcement, reactive oxygen species-mediated activated all accessions compared susceptible accession. Unique responses accession integrate early reinforcement structural barriers with sustained chemical defences stress responses. Overall, exhibited front-loaded focused on biotic stimuli interspecies interactions infection stages. In contrast, partial weaker baseline system, slower less robust response specifically targeting pathogen infection. We identified potential effectors, hydrolysis, nutrient acquisition, virulence, higher diversity effectors This study identifies novel targets within complex sativus-Erysiphe interaction that will support future breeding programs aimed enhancing other related species.

Язык: Английский

Процитировано

0