Mechanistic Exploration of Shugan Jianpi Formula for Treating Triple-Negative Breast Cancer Under Chronic Stress: A Network Pharmacology-Guided Experimental Study DOI Creative Commons
Fen Liu,

L. T. Li,

Yiming Zhang

и другие.

Drug Design Development and Therapy, Год журнала: 2025, Номер Volume 19, С. 4585 - 4603

Опубликована: Май 1, 2025

This study aimed to investigate the pharmacological mechanisms of Shugan Jianpi Formula (SGJPF) in treating TNBC using network pharmacology and molecular biology approaches. HPLC/MS identified key compounds SGJPF. In vitro assays were performed on norepinephrine (NE)-stimulated MDA-MB-231 SUM159PT cells mimic triple-negative breast cancer (TNBC) under chronic psychological stress (CPS) evaluate SGJPF's effects cell proliferation, apoptosis, cycle, migration, invasion. A mouse model exposed CPS was used assess influence tumor growth. explored via docking, with target validation through Western blotting, immunohistochemistry, immunofluorescence. analysis 806 SGJPF, including flavonoids, polyphenols, saponins, polysaccharides, alkaloids, terpenoids, coumarins, organic acids, glycosides. Network docking analyses SRC, ERK (MAPK1), STAT3 as pivotal targets underlying anti-tumor SGJPF TNBC. Both vivo experiments confirmed that exerts its therapeutic modulation SRC/ERK/STAT3 signaling axis. vitro, effectively inhibited invasion, while promoting apoptosis NE-stimulated cells. a CPS-induced model, significantly alleviated progression, further corroborating potential novel strategy for highlights axis, offering robust foundation investigation into clinical application.

Язык: Английский

Invasion and metastasis in cancer: molecular insights and therapeutic targets DOI Creative Commons

Yongxing Li,

Fengshuo Liu,

Qingjin Cai

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2025, Номер 10(1)

Опубликована: Фев. 20, 2025

The progression of malignant tumors leads to the development secondary in various organs, including bones, brain, liver, and lungs. This metastatic process severely impacts prognosis patients, significantly affecting their quality life survival rates. Research efforts have consistently focused on intricate mechanisms underlying this corresponding clinical management strategies. Consequently, a comprehensive understanding biological foundations tumor metastasis, identification pivotal signaling pathways, systematic evaluation existing emerging therapeutic strategies are paramount enhancing overall diagnostic treatment capabilities for tumors. However, current research is primarily metastasis within specific cancer types, leaving significant gaps our complex cascade, organ-specific tropism mechanisms, targeted treatments. In study, we examine sequential processes elucidate driving organ-tropic systematically analyze tumors, those tailored organ involvement. Subsequently, synthesize most recent advances technologies challenges opportunities encountered pertaining bone metastasis. Our objective offer insights that can inform future practice crucial field.

Язык: Английский

Процитировано

7
Urchin-like magnetic nanoparticles loaded with type X collagen siRNA and Stattic to treat triple negative breast cancer under rotating magnetic field like an “enchanted micro-scalpel” DOI
Jie Liu,

Xiao-Rong Qiu,

Yi-Le Tian

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 300, С. 140318 - 140318

Опубликована: Янв. 27, 2025

Язык: Английский

Процитировано

0
Identification of Common Angiogenesis Marker Genes in Chronic Lung Diseases and Their Relationship with Immune Infiltration Based on Bioinformatics Approaches DOI Creative Commons
Lu Liu, Man Wang,

Shihuan Yu

и другие.

Biomedicines, Год журнала: 2025, Номер 13(2), С. 331 - 331

Опубликована: Янв. 31, 2025

Objective: This study aims to explore the role of angiogenesis-related genes in chronic lung diseases (ILD and COPD) using bioinformatics methods, with goal identifying novel therapeutic targets slow disease progression prevent its deterioration into fibrosis or pulmonary artery hypertension. Methods: The research methods encompassed differential analysis, WGCNA (Weighted Gene Co-expression Network Analysis), multiple machine learning approaches screen for key genes. Set Enrichment Analysis (GSEA), Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) were utilized assess related biological functions pathways. Additionally, immune cell infiltration was analyzed evaluate status correlation between immunity. Results: COPD ILD are closely associated pathways angiogenesis, responses, others, both groups linked inflammation-related signaling established a disease-related gene set comprising 171 further screened out 21 angiogenesis. Ultimately, four genes—COL10A1, EDN1, MMP1, RRAS—were identified through methods. These angiogenesis processes, clustering analysis based on them can reflect different states variations infiltration. Conclusions: COL10A1, RRAS represent potential slowing preventing their deterioration. Furthermore, monocytes exhibited consistent patterns across control groups, as well among subgroups, suggesting significant development diseases.

Язык: Английский

Процитировано

0
Mechanistic Exploration of Shugan Jianpi Formula for Treating Triple-Negative Breast Cancer Under Chronic Stress: A Network Pharmacology-Guided Experimental Study DOI Creative Commons
Fen Liu,

L. T. Li,

Yiming Zhang

и другие.

Drug Design Development and Therapy, Год журнала: 2025, Номер Volume 19, С. 4585 - 4603

Опубликована: Май 1, 2025

This study aimed to investigate the pharmacological mechanisms of Shugan Jianpi Formula (SGJPF) in treating TNBC using network pharmacology and molecular biology approaches. HPLC/MS identified key compounds SGJPF. In vitro assays were performed on norepinephrine (NE)-stimulated MDA-MB-231 SUM159PT cells mimic triple-negative breast cancer (TNBC) under chronic psychological stress (CPS) evaluate SGJPF's effects cell proliferation, apoptosis, cycle, migration, invasion. A mouse model exposed CPS was used assess influence tumor growth. explored via docking, with target validation through Western blotting, immunohistochemistry, immunofluorescence. analysis 806 SGJPF, including flavonoids, polyphenols, saponins, polysaccharides, alkaloids, terpenoids, coumarins, organic acids, glycosides. Network docking analyses SRC, ERK (MAPK1), STAT3 as pivotal targets underlying anti-tumor SGJPF TNBC. Both vivo experiments confirmed that exerts its therapeutic modulation SRC/ERK/STAT3 signaling axis. vitro, effectively inhibited invasion, while promoting apoptosis NE-stimulated cells. a CPS-induced model, significantly alleviated progression, further corroborating potential novel strategy for highlights axis, offering robust foundation investigation into clinical application.

Язык: Английский

Процитировано

0