Critical Reviews in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 1 - 26

Опубликована: Окт. 3, 2024

Immunoassays based on the specific antigen-antibody interactions are efficient tools to detect various compounds and estimate their content. Usually, these assays implemented in water-saline media with composition close physiological conditions. However, many substances insoluble or cannot be molecularly dispersed such media, which objectively creates problems when interacting aquatic environments. Thus, obtaining immunoreactants implementing immunoassays of need special methodological solutions. Hydrophobicity antigens as well limited ability functionalization conjugation often overlooked developing for compounds. The main key finding is possibility influence behavior hydrophobic immunoassays, requires approaches summarized review. Using examples two groups compounds-surfactants (alkyl- bisphenols) fullerenes, we systematized existing knowledge experience development immunoassays. This review addresses challenges immunodetection poorly soluble proposes solutions use hydrotropes, other solubilization techniques, alternative receptors (aptamers imprinted polymers).

Язык: Английский