Development of a consolidated bioprocess for the production of citric acid using Aspergillus niger as biocatalyst DOI Creative Commons
Ludovica Varriale, Katrin Kuka, N. Tippkötter

и другие.

Biofuels Bioproducts and Biorefining, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Abstract The industrial production of citric acid, an ingredient in beverages, pharmaceuticals, and cosmetics, is based on microbial fermentation glucose or sucrose. Given the elevated cost these sugars, lignocellulosic biomass emerging as a cost‐effective environmentally friendly feedstock for sustainable bioprocesses. However, materials requires that they are first broken down enzymatically. This can be achieved by filamentous fungus Aspergillus niger , which has ability to secrete hydrolytic enzymes produce acid. Here, we investigated acid using consolidated bioprocess, all conversion steps – from solid substrate final product occurred single process stage. press cake derived perennial ryegrass ( Lolium perenne ) was used remaining juice were utilized additional carbon source. produced citrate successfully only when supplemented with (2.1 ± 0.0 g kg DM −1 especially (84.7 0.3 ). Confocal laser scanning microscopy revealed differences fungal mycelia source supplemented. Overall, results indicate successful implementation solid‐state A. fed cake.

Язык: Английский

Novel fermentation strategy utilizing a probiotic complex to improve the feeding value of distillers’ grains DOI Creative Commons
Zhiwei Wang, Yangguang Li, Haoran Tang

и другие.

Industrial Crops and Products, Год журнала: 2025, Номер 226, С. 120748 - 120748

Опубликована: Фев. 20, 2025

Язык: Английский

Процитировано

0

Trends in Enzyme Production from Citrus By-Products DOI Open Access
Caio de Azevedo Lima, Alex Graça Contato, Fernanda de Oliveira

и другие.

Processes, Год журнала: 2025, Номер 13(3), С. 766 - 766

Опубликована: Март 6, 2025

Citrus fruit production generates substantial by-products, primarily from juice processing, which represent significant environmental and economic challenges. However, these residues, rich in polysaccharides, flavonoids, essential oils, enzymes, offer an untapped resource for biotechnological applications. This review explores the potential of citrus by-products as substrates enzyme production, focusing on key industrial enzymes such cellulases, pectinases, xylanases, ligninases, lipases, proteases. Various microbial strains have demonstrated ability to convert residues into high-value through solid-state submerged fermentation. The optimization fermentation conditions—including temperature, pH, moisture content, carbon-to-nitrogen ratio—further enhances enzymatic yields. valorization waste aligns with circular economy principles, reducing impacts while supporting sustainable bioproduct development food, biofuel, pharmaceutical, textile industries. Future research should focus scaling up using improve feasibility advance biorefineries.

Язык: Английский

Процитировано

0

Development of a consolidated bioprocess for the production of citric acid using Aspergillus niger as biocatalyst DOI Creative Commons
Ludovica Varriale, Katrin Kuka, N. Tippkötter

и другие.

Biofuels Bioproducts and Biorefining, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Abstract The industrial production of citric acid, an ingredient in beverages, pharmaceuticals, and cosmetics, is based on microbial fermentation glucose or sucrose. Given the elevated cost these sugars, lignocellulosic biomass emerging as a cost‐effective environmentally friendly feedstock for sustainable bioprocesses. However, materials requires that they are first broken down enzymatically. This can be achieved by filamentous fungus Aspergillus niger , which has ability to secrete hydrolytic enzymes produce acid. Here, we investigated acid using consolidated bioprocess, all conversion steps – from solid substrate final product occurred single process stage. press cake derived perennial ryegrass ( Lolium perenne ) was used remaining juice were utilized additional carbon source. produced citrate successfully only when supplemented with (2.1 ± 0.0 g kg DM −1 especially (84.7 0.3 ). Confocal laser scanning microscopy revealed differences fungal mycelia source supplemented. Overall, results indicate successful implementation solid‐state A. fed cake.

Язык: Английский

Процитировано

0