BMC Complementary Medicine and Therapies, Год журнала: 2025, Номер 25(1)
Опубликована: Март 8, 2025
Shilajit is a pale-brown to blackish-brown fluid that varies in consistency and released from rock layers various mountain ranges across the world. For thousands of years, traditional medical systems several nations have included shilajit one form or another as rejuvenator adaptogen. Numerous medicinal qualities been attributed it, which confirmed by contemporary scientific analysis. This vitro study was established investigate effect on human Periodontal ligament (hPDL) cell wound closure. The viability evaluated using 3-(4,5-dimethylthiazol-2-yl)—2,5-diphenyltetrazolium bromide tetrazolium reduction (MTT) test following 24-hour exposure shilajit. With use an inverted phase contrast microscope, morphological alterations were noted. Acridine orange/ethidium dual labeling, evaluate apoptotic death treated cells. An healing utilized wounded hPDL monolayers for 24 h presence absence Matrix metalloproteinases-2 9 (MMP-2 MMP-9) cells with measured enzyme-linked immunosorbent assay (ELISA) technique, real-time PCR used examine gene expression linked apoptosis. Shilajit's cytotoxicity evaluation showed dosages high 3 mg/mL had no adverse effects maintains viability, suggesting possible stimulatory growth. Cell reduced significantly (p < 0.05) more than 4 mg/mL, indicating at higher concentrations. According scratch assay, administration doses 2 accelerated improved migration promoted controlled inflammatory response supported periodontal upregulating genes involved collagen synthesis, type I alpha 1 chain (COL1A1) pro-inflammatory cytokines, tumor necrosis factor (TNF-α) and, interleukin-1-beta (IL-1β), according data. In addition, raised protein levels MMP2 MMP9, two important enzymes tissue remodeling. Shilajit-treated substantial increase proliferation discernible activity. Our research offers novel proof promotes proliferation, turn these results, may improve regeneration, accelerate healing, encourage growth
Язык: Английский