Transcriptomic and metabolomic analyses reveal the mechanism of color difference between two kinds of Cistanche deserticola before and after drying

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Янв. 23, 2025

Introduction Cistanche deserticola is an important traditional Chinese herbal medicine. The fresh cistanche squamous stem typically yellow-white and brown after drying. Oil a variant with purple that turns black color difference between oil obvious, the former has higher market price. However, mechanism underlying of remains unclear. Methods This study evaluated total flavone contents in compared differential metabolites differentially expressed genes (DEGs) iridoid dried samples were determined by high-performance liquid chromatography, finally polysaccharides them to comprehensively analyze formation cistanche. Results results showed flavonoid content was significantly than Metabolomic analysis identified 50 accumulated (DAMs) (34 up-regulated 16 down-regulated), including carbohydrates, terpenoids, flavonoids. Moreover, 3,376 DEGs selected, among which significant IGS1 CYP84A1 down-regulated 4CLL1 , F6H2-2-1 5MAT1 jointly regulated biosynthesis affected accumulation metabolites. Significant CCD7 gene carotenoid component production, UGT85A24 promoted geniposidic acid. In addition, polysaccharide those Discussion expression flavonoids terpenoid FLS mainly caused Dried darker cistanche, due iridoids former. preliminarily revealed causes differences provided references for systematic its germplasm resources, as well breeding C. .

Язык: Английский

Optimization of Extraction Process, Structural Characterization, and Antioxidant and Hypoglycemic Activity Evaluation of Polysaccharides From the Medicinal and Edible Plant: Cistanche deserticola Ma

Phytochemical Analysis, Год журнала: 2025, Номер unknown

Опубликована: Янв. 23, 2025

ABSTRACT Introduction Cistanche deserticola Ma (CD), an edible and medicinal plant native to Xinjiang, Inner Mongolia, Gansu in China, is rich bioactive polysaccharides known for their health‐promoting properties. The of C. (CDPs) have been shown possess a range beneficial activities, including immunomodulatory, anti‐aging, antioxidant, anti‐osteoporosis effects. Objective This study seeks identify the optimal conditions extracting CDPs using hot water. Additionally, it aims evaluate chemical properties, antioxidant activity, hypoglycemic effects, cytotoxicity. findings will provide theoretical foundation potential use functional foods pharmaceuticals. Methodology employed response surface methodology optimize water extraction CDPs. extracted were characterized chemical, spectroscopic, instrumental analyses. Furthermore, cytotoxicity evaluated through relevant assays assess health benefits. Results Under conditions, yield was 45.85% ± 1.91%. identified as acidic heteropolysaccharides with wide molecular weight distribution, ranging from 0.3 128.2 kDa. They composed primarily glucose (51.21%), arabinose (32.86%), galactose (17.88%), smaller amounts galacturonic acid (4.66%), rhamnose (1.85%), mannose (1.32%), glucosamine hydrochloride (1.08%), xylose (0.56%). Antioxidant demonstrated that exhibited significant free radical scavenging metal ion chelation, reducing power. inhibited α‐glucosidase α‐amylase vitro mixed‐type mechanism, well static fluorescence quenching. Cytotoxicity showed nontoxic L02 AML12 cells. Conclusion offers pharmaceuticals provides valuable insights development new agents natural sources.

Язык: Английский

Lactiplantibacillus plantarum LZU-J-Q21 enhanced the functional metabolic profile and bioactivity of Cistanche deserticola

Food Chemistry X, Год журнала: 2024, Номер 24, С. 101941 - 101941

Опубликована: Окт. 31, 2024

Язык: Английский