Comprehensive MALDI mass spectrometry imaging of tumor regions post-neoadjuvant therapy
Analytical and Bioanalytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 20, 2025
Язык: Английский
Unveiling purine metabolism dysregulation orchestrated immunosuppression in advanced pancreatic cancer and concentrating on the central role of NT5E
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 1, 2025
The
dismal
efficacy
of
immunotherapy
for
Pancreatic
cancer
(PC)
can
be
predominantly
ascribed
to
its
distinctive
cold-tumor
properties.
by-products
purine
metabolic
reprogramming
are
extensively
engaged
in
tumor
immune
modulation,
influencing
the
functions
and
recruitment
cells
molding
an
microenvironment
that
is
propitious
growth.
We
harnessed
single-cell
transcriptomics
spatial
concurrently
analyze
metabolism
(PM)
features
PC
microenvironment.
quantitatively
appraised
PM
traits
diverse
cell
subsets
via
scoring
algorithms
such
as
AUCell
Ucell.
Moreover,
development
cell-cell
interaction
analysis
elucidated
alterations
TME
induced
by
dysregulation.
Additionally,
we
defined
disorder
characteristics
patients
utilized
this
assess
phenotypes
prognoses
patient
population.
Also,
identified
crucial
intermediate
genes
impact
establishment
immunosuppressive
environment
within
PC,
validated
them
through
sectioning
co-culture
experiments.
Multi
-
dimensional
transcriptome
data
unique
heterogeneity
microenvironment,
which
manifested
fibroblasts
demonstrating
higher
scores
TME.
Cellchat
revealed
malignant
with
elevated
expression
were
concomitantly
associated
frequent
interactions
CAFs
well
high
ligand-receptor
pairs
transcription
factors.
Spatial
further
corroborated
finding.
Furthermore,
newly
constructed
criteria
indicated
levels
a
lack
response
Finally,
study
singular
role
NT5E
immunosuppression
resulting
from
PC.
CCK8
invasion
experiments
following
model
demonstrated
intervention
targeting
could
reverse
augmented
malignancy
co-cultured
CAFs.
potentially
key
target
reversing
"stiff-cancer"
This
demonstrates
disorders
impinge
upon
exacerbate
engendered
progression
fibrosis.
Therapeutic
strategies
or
may
offer
ray
hope
advanced
PDAC.
Язык: Английский
Purine Metabolism Pathway Influence on Running Capacity in Rats
Dengbo Chen,
Christian Noble Biney,
Qian Wang
и другие.
Metabolites,
Год журнала:
2025,
Номер
15(4), С. 241 - 241
Опубликована: Апрель 2, 2025
Background:
The
natural
differences
in
running
capacities
among
rats
remain
poorly
understood,
and
the
mechanisms
driving
these
need
further
investigation.
Methods:
Twenty
male
Sprague-Dawley
(SD)
were
selected.
High
low
capacity
identified
using
Treadmill
Exhaustion
Tests.
Peripheral
blood
was
collected
for
serum
isolation,
followed
by
a
metabolomics
analysis
LC-MS/MS.
Data
preprocessed,
principal
component
(PCA)
partial
least
squares-discriminant
(PLS-DA)
applied
to
identify
metabolic
profile
differences.
Significant
metabolites
screened,
pathway
enrichment
conducted
KEGG
database
determine
key
pathways.
Forty
SD
(equal
female)
randomly
divided
into
an
inosine
triphosphate
(ITP)
group
(24.29
mg/kg.bw
daily)
control
group.
Running
assessed
after
one
week
of
continuous
treatment.
Results:
Three
independent
measurements
showed
consistent
capacity.
A
total
519
differential
identified,
with
255
up-regulated
264
down-regulated.
revealed
significant
Purine
Metabolism
(ITP-ATP)
high
(p
<
0.05).
ITP-treated
exhibited
significantly
higher
than
controls
0.05),
confirming
efficacy
dietary
ITP
supplementation.
Conclusions:
is
influenced
ITP-ATP
pathway,
exogenous
administration
through
intervention
improves
ability.
Язык: Английский
iTRAQ-Based Proteomic Profiling of Skin Aging Protective Effects of Tremella fuciformis-Derived Polysaccharides on D-Galactose-Induced Aging Mice
Molecules,
Год журнала:
2024,
Номер
29(21), С. 5191 - 5191
Опубликована: Ноя. 2, 2024
In
the
present
study,
a
heteromannan
primarily
composed
of
mannose,
fucose,
xylose,
glucose,
and
arabinose
at
molar
ratio
4.78:1.18:1:0.82:0.11
containing
low
proportion
glucuronic
acid
with
weight-average
molecular
weights
3.6
×
106
Da,
named
NTP,
was
prepared
from
fruiting
body
Tremella
fuciformis.
The
anti-skin-aging
effects
NTP
on
d-Galactose-induced
aging
mice
biological
mechanisms
were
investigated
by
an
iTRAQ-based
proteomics
approach.
substantially
mitigated
skin
characterized
decreased
loss
hydroxyproline
hyaluronic
reduced
oxidative
stress
in
skin.
Moreover,
43
differentially
expressed
proteins
(DEPs)
identified
response
to
which
23
up-regulated
20
down-regulated.
Bioinformatics
analysis
revealed
that
these
DEPs
mainly
involved
functions
cellular
metabolic
regulations,
immune
system
responses,
structural
components.
findings
provided
new
insights
into
underlying
actions
T.
fuciformis-derived
polysaccharides
facilitated
applications
naturally
functional
foods.
Язык: Английский