Fabrication and Characterization of Electrospun DegraPol® Tubes Releasing TIMP-1 Protein to Modulate Tendon Healing

Materials, Год журнала: 2025, Номер 18(3), С. 665 - 665

Опубликована: Фев. 3, 2025

Background: Tendon rupture repair can result from fibrotic scar formation through imbalanced ECM deposition during remodeling. The tissue inhibitors of matrix metalloprotease (TIMPs) not only decrease degradation, regulated by metalloproteases (MMPs), but also restrict TGF-β1 activation and thus diminish fibrosis. Methods: Rabbit tenocytes (rbTenocytes) rabbit adipose-derived stem cells (rbASCs) were cultivated under different TIMP-1 concentrations. Proliferation gene expression assessed. was incorporated into emulsion electrospun DegraPol® (DP) tubes that characterized SEM for fiber thickness, pore size, wall thickness. Static dynamic water contact angles, FTIR spectra, release kinetics determined. Results: While the proliferation rbTenocytes rbACS affected supplementation in vitro, Col1A1 increased rbTenocytes, ki67 both cell types, tenomodulin types at 100 ng/mL TIMP-1, alkaline phosphatase ALP rose significantly rbASCs. Electrospun TIMP-1/DP fibers had a ~5 μm diameter, ~10 mesh thickness ~200 μm. meshes more hydrophilic than pure DP meshes. released with sustained up to 7 days. Conclusions: may be used modulate reaction when applied around conventionally sutured tendon ruptures.

Язык: Английский

Research Progress on the Preparation and Application of Decellularized Tendons

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(4), С. 251 - 251

Опубликована: Апрель 6, 2025

Tendons connect animal skeletons to skeletal muscles, playing a crucial role in weight-bearing and maintaining motor functions. After decellularization, tendon extracellular matrix (tECM) retains the physicochemical characteristics similar those of native tendons. This has made tECM promising biomaterial fields tissue engineering regenerative medicine recent years. paper summarizes origin, structure, ECM components tendons, reviews decellularization methods, discusses advancements research applications decellularized Furthermore, it explores future development trends xenogeneic materials, aiming provide reference for fundamental biomaterials related

Язык: Английский

Tendon injury repair strategy driven by supramolecular hydrogen bonding and dynamic covalent bond self-assembly microspheres

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162726 - 162726

Опубликована: Апрель 1, 2025

Язык: Английский