Fe3+ Reducing Power as the Most Common Assay for Understanding the Biological Functions of Antioxidants DOI Open Access
İlhami Gülçın, Saleh Alwasel

Processes, Год журнала: 2025, Номер 13(5), С. 1296 - 1296

Опубликована: Апрель 24, 2025

Antioxidants counteract the harmful effects of free radicals on metabolism and prevent fatty food degradation during processing storage. The Fe3+-reducing assay, based reduction ferric ions (Fe3+) to ferrous (Fe2+) in presence antioxidants acting as reducing agents, is widely recognized used evaluate antioxidant capacity various biological samples, including plant extracts, food, beverages, pharmaceuticals. Reduction Fe3+ Fe2+ also crucial biogeochemical cycling, microbial metabolism, industrial applications. This review comprehensively describes its adaptation different analytes, identification most potent antioxidants, optimization measurement techniques. It outlines chemical fundamental principles ability, along with an in-depth analysis activity, covering biochemical mechanisms, contributions, analytical methods, practical applications recent advances future perspectives research. assay straightforward, testing compounds or extracts are mixed solution, their absorbance measured after a specific incubation period. Despite significant advancements instrumentation techniques, this method remains largely unchanged.

Язык: Английский

Structure Characterization and Antioxidant Activity of a Novel Polysaccharide from Bacillus natto Fermented Millet Bran DOI Creative Commons
Hongsheng Zhang,

Xia Fan,

Wenjie Zhao

и другие.

Foods, Год журнала: 2025, Номер 14(2), С. 278 - 278

Опубликована: Янв. 16, 2025

To improve the high-value application of millet bran, a water-soluble polysaccharide was extracted from fermented bran (FMBP) by using Bacillus natto fermentation. A neutral polysaccharide, FMBP-1, separated and purified FMBP an anion exchange column. Its structure antioxidant activity in vitro were characterized determined. The molecular weight FMBP-1 1.154 × 104 Da, its distribution relatively uniform. monosaccharide composition, FT-IR, methylation, NMR results indicated that only composed glucose α-(1→4)-D-glucan branched at O-6 with terminal 1-linked α-D-Glcp as side chain. In addition, assays possessed certain capacities for scavenging free radicals reducing power, this concentration-dependent manner. This research will provide fundamental data regarding structure–activity relationship polysaccharides theoretical foundation utilization within food pharmaceutical industries.

Язык: Английский

Процитировано

0

Physicochemical Properties and Cytoprotective Effects on PC12 Cells of Polysaccharides from Belamcanda chinensis (L.) DC. Obtained via a Gradient Ethanol Precipitation Method DOI Creative Commons

Yuanqi Duan,

Jinfeng Sun, Yongkang Xue

и другие.

Molecules, Год журнала: 2025, Номер 30(5), С. 998 - 998

Опубликована: Фев. 21, 2025

Given that the preparation method of polysaccharides affects functional properties, four types acidic (BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a) were prepared using gradient ethanol precipitation method. Then, a series chemical instrumental analysis techniques used to compare structural characteristics morphology. Neuroprotective effects explored OGD/R-induced PC12 cells. The results showed BCP30-1a, BCP90-1a had similar characteristic groups contained both β-glycosidic α-glycosidic bonds. Their molecular weights, in descending order, 198.398 kDa, 184.690 184.556 184.217 respectively. In addition, different proportions glycosidic bonds, namely, Manp-(1→, →5)-Araf-(1→, →3)-Galp (or GalAp)-(1→, →4)-Glcp-(1→ →3,6)-Galp-(1→. BCP30-1a also certain proportion Galp-(1→, each polysaccharide microscopic good thermal stability. Finally, exhibited cytoprotective on cells based OGD/R model. These findings provide novel regulatory strategy for activity BCPs offer scientific evidence supporting application research field ischemic stroke.

Язык: Английский

Процитировано

0

Pioneering polysaccharide extraction with deep eutectic solvents: A review on impacts to extraction yield, physicochemical properties and bioactivities DOI
Muhammad Hasnun Md Yusoff, Muhammad Hakimin Shafie

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 306, С. 141469 - 141469

Опубликована: Фев. 25, 2025

Язык: Английский

Процитировано

0

Steam explosion improved the physicochemical properties, hypoglycemic effects of polysaccharides from Clerodendranthus spicatus leaf via regulating IRS1/PI3K/AKT/GSK-3β signaling pathway in IR-HepG2 cells DOI

Xiaoyu Zhang,

Tingting Zhang, Jia Wang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142586 - 142586

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

A polysaccharide PFH-3a from Fritillariae hupehensis bulbus: Preparation, structural characterization and antioxidant activity in vitro DOI

Jiang Xin,

Junyin Zhang, Caihong Li

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 143210 - 143210

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Comparative Analysis of Three Extraction Methods for Ginger Leaf Polysaccharide: Chemical Composition, Antioxidant activity, and Hypolipidemic Effects DOI
Zhaohui Zhang,

Zhao Zhong-hua,

Tianyi Cao

и другие.

Journal of Applied Research on Medicinal and Aromatic Plants, Год журнала: 2025, Номер unknown, С. 100639 - 100639

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Fe3+ Reducing Power as the Most Common Assay for Understanding the Biological Functions of Antioxidants DOI Open Access
İlhami Gülçın, Saleh Alwasel

Processes, Год журнала: 2025, Номер 13(5), С. 1296 - 1296

Опубликована: Апрель 24, 2025

Antioxidants counteract the harmful effects of free radicals on metabolism and prevent fatty food degradation during processing storage. The Fe3+-reducing assay, based reduction ferric ions (Fe3+) to ferrous (Fe2+) in presence antioxidants acting as reducing agents, is widely recognized used evaluate antioxidant capacity various biological samples, including plant extracts, food, beverages, pharmaceuticals. Reduction Fe3+ Fe2+ also crucial biogeochemical cycling, microbial metabolism, industrial applications. This review comprehensively describes its adaptation different analytes, identification most potent antioxidants, optimization measurement techniques. It outlines chemical fundamental principles ability, along with an in-depth analysis activity, covering biochemical mechanisms, contributions, analytical methods, practical applications recent advances future perspectives research. assay straightforward, testing compounds or extracts are mixed solution, their absorbance measured after a specific incubation period. Despite significant advancements instrumentation techniques, this method remains largely unchanged.

Язык: Английский

Процитировано

0