Comprehensive insights and In silico analysis into the emerging role of LincRNAs in lung diseases pathogenesis; a step toward ncRNA precision
Functional & Integrative Genomics,
Год журнала:
2025,
Номер
25(1)
Опубликована: Фев. 6, 2025
Abstract
Long
non-coding
RNAs
(lncRNAs)
have
emerged
as
essential
regulators
of
gene
expression,
significantly
influencing
various
biological
processes.
Approximately
half
all
lncRNAs
are
classified
long
intergenic
(lincRNAs),
which
situated
among
coding
genes.
Recent
studies
documented
the
role
lincRNAs
in
pathogenesis
lung
diseases,
including
cancer,
pulmonary
fibrosis,
and
arterial
hypertension.
These
can
modulate
expression
through
mechanisms,
epigenetic
modifications,
transcriptional
regulation,
post-transcriptional
regulation.
By
functioning
competing
endogenous
(ceRNAs),
affect
activity
microRNAs
(miRNAs)
their
corresponding
target
This
review
delves
into
intricate
mechanisms
by
contribute
to
development
progression
diseases.
Furthermore,
it
discusses
potential
therapeutic
targets.
Язык: Английский
Distinct 5-methylcytosine profiles of LncRNA in breast cancer brain metastasis
BMC Cancer,
Год журнала:
2025,
Номер
25(1)
Опубликована: Март 27, 2025
Recent
studies
have
identified
a
complex
relationship
between
methylation
patterns
and
the
development
of
various
cancers.
Breast
cancer
(BC)
is
second
leading
cause
mortality
among
women.
Approximately
5–20%
BC
patients
are
at
risk
brain
metastases
(BCBM).
Although
5-methylcytosine
(m5C)
has
been
as
an
important
regulatory
modifier,
its
distribution
in
BCBM
not
well
understood.
This
study
aimed
to
investigate
m5C
BCBM.
Samples
from
(231-BR
cells)
(MDA-MB-231
groups
were
subjected
comprehensive
analysis
long
non-coding
RNA
(lncRNA)
using
methylated
immunoprecipitation
next-generation
sequencing
(MeRIP-seq).
The
expression
levels
genes
adjacent
tissues
verified
through
quantitative
real-time
polymerase
chain
reaction
(RT-qPCR).
Enrichment
pathway
analyses
Gene
Ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
predict
potential
functions
MeRIP-seq
23,934
peaks
21,236
BC.
A
total
9,480
annotated
(BCBM)
8,481
mapped.
Notably,
1,819
sites
lncRNA
upregulated
BCBM,
whereas
2,415
Significant
hypermethylated
lncRNAs
included
ENST00000477316,
ENST00000478098
uc002gtt.1,
hypomethylated
ENST00000600912,
ENST00000493668,
ENST00000544651
ENST00000464989.
These
results
by
qPCR
MeRIP-qPCR
Considering
strong
association
regulators
lncRNA,
we
examined
13
observed
significant
differences
normal
tissues.
In
addition,
interaction
altered
differentially
expressed
vitro
was
analyzed.
GO
KEGG
pathways
revealed
that
significantly
associated
with
linked
signaling
pathways.
uncovered
variations
compared
findings
provide
new
theoretical
understanding
mechanisms
Язык: Английский
Serum-derived exosomal miR-7977 combined with miR-451a as a potential biomarker for pancreatic ductal adenocarcinoma
BMC Cancer,
Год журнала:
2025,
Номер
25(1)
Опубликована: Фев. 19, 2025
To
explore
the
potential
of
serum
exosomal
miRNAs
as
novel
biomarkers
for
pancreatic
ductal
adenocarcinoma
(PDAC).
Serum
were
screened
and
verified
by
microarray
analysis
quantitative
real-time
PCR
(qRT-PCR)
in
patients
with
PDAC
healthy
controls.
The
correlation
between
clinical
characteristics
candidate
was
analyzed,
diagnostic
performance
evaluated.
miR-7977
miR-451a
significantly
upregulated
compared
controls,
levels
exosomes
closely
associated
stage
metastasis
patients.
area
under
curve
(AUC)
values
0.825
0.804
training
set
0.796
0.830
validation
set,
respectively.
A
biomarker
panel
consisting
these
two
resulted
a
power
an
AUC
0.901
0.918
set.
might
be
PDAC.
These
miRNAs,
when
combined,
exhibit
optimal
performance.
Язык: Английский
piRNAs and circRNAs acting as diagnostic biomarkers in clear cell renal cell carcinoma
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 5, 2025
The
discovery
of
diverse
functions
and
mechanisms
in
cancer
has
underscored
the
significance
emerging
non-coding
RNAs
(ncRNAs),
such
as
PIWI-interacting
(piRNAs)
circular
(circRNAs),
within
clinical
context
cancer.
Understanding
their
role
clear
cell
renal
carcinoma
(ccRCC)
is
imperative
necessitates
comprehensive
investigation.
This
study
aims
to
further
explore
diagnostic
potential
piRNAs
circRNAs
for
ccRCC.
dysregulated
ccRCC
were
identified
using
small
RNA
(sRNA)
high-throughput
sequencing
technology,
while
expression
samples
was
assessed
by
RT-qPCR.
A
paired
t-test
performed
compare
levels
between
adjacent
tissues.
Additionally,
ROC
curve
analysis
conducted
evaluate
specificity,
sensitivity,
area
under
(AUC)
circRNAs.
High-throughput
revealed
a
significant
downregulation
17
694
tissues,
accompanied
upregulation
5
490
RT-qPCR
demonstrated
markedly
lower
piR-has-150997,
133872,
132556,
154502,
uniq-84737
group
compared
tissue
(p
<
0.05).
When
considering
combined
detection
piR-hsa-150997,
piR-hsa-133872,
piR-hsa-132556,
piR-hsa-154502,
uniq_84737,
circABCC1,
circNETO2_006,
circARID1B_037,
AUC
found
be
high
at
an
approximate
value
=
0.878.
performance
uniq-84737,
circARID1B_037
demonstrates
promise
model
incorporating
could
serve
ideal
marker
system
with
utility.
Язык: Английский