Fluorescence detection for enrofloxacin by using Aptamer and fluorophore SYBR Green I DOI

Chenchen Li,

Wei‐Chuang Kong,

Jia-Le Cui

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 11, 2024

Abstract Enrofloxacin (ENR), as a type of antimicrobial, is commonly used in animal sterilization. However, excessive ENR residues food will seriously harm human health. Therefore, this work, we developed quantitative fluorescence method for based on aptamer and SYBR Green I (SG-I). DNA aptamers with G-quadruplex space structure can be recognized inserted by SG-I to emit intense green fluorescence. In the presence ENR, preferentially bound specifically resulting change conformation, which prevented from inserting into cause quenching. The sensor quantitatively detect linear concentration range 0-600 nM, limit detection was 0.158 nM. detecting chicken, fish pork samples recovery rate 99.74–104.02%, relative standard deviation (RSD) less than 6%. aptamer-based provided safety.

Язык: Английский

Recent Advances in Electrochemical Aptasensors for Detection of Clinical and Veterinary Drugs DOI

Masoomeh Naseri,

Али Ниази, Atisa Yazdanipour

и другие.

Critical Reviews in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 1 - 15

Опубликована: Март 18, 2025

Nowadays, aptamer-based biosensors and electrochemical measurements represent one of the efficient tools for detection drugs in both medical veterinary. Precise trace values analysis chemicals, especially drugs, plays a crucial role food therapeutic safety evaluations that are often time-consuming costly. Ultimately, accurate determination medications like antibiotics food, environmental resources, biological matrices is very important protecting public health drug monitoring (TDM) effective treatment. This review highlights recent advancements aptasensors as an innovative approach offering high sensitivity, specificity, rapid clinical veterinary at lower costs. We provide comprehensive overview discuss challenges prospects aptasensing residue across various samples.

Язык: Английский

Процитировано

0
A label-free aptasensor based on a fluorescent probe containing styrylbenzothiazole for detection of enrofloxacin DOI

Wei‐Chuang Kong,

Chenchen Li,

Juntao Niu

и другие.

Analytical Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

We have successfully constructed a fluorescent aptasensor based on an ENR aptamer and the probe PBTZM containing styrylbenzothiazole that enabled rapid, label-free detection of with high sensitivity.

Язык: Английский

Процитировано

0
Bio-inspired Synthesis of Silver and Zinc Oxide Nanoparticles Using Syzygium salicifolium: Unveiling Versatile In Vitro Biomedical and Photocatalytic Applications DOI
Annie Princy Nadar, Krishnan Sellappan

Journal of Cluster Science, Год журнала: 2025, Номер 36(3)

Опубликована: Апрель 25, 2025

Язык: Английский

Процитировано

0
Exo Ⅲ-assisted amplification signal strategy synergized with Au@Pt NFs/CoSe2 for sensitive detection of enrofloxacin DOI

Xiaoying Cui,

Lina Lv, Ke Zhao

и другие.

Bioelectrochemistry, Год журнала: 2024, Номер 160, С. 108750 - 108750

Опубликована: Май 28, 2024

Язык: Английский

Процитировано

2
Fluorescence detection for enrofloxacin by using Aptamer and fluorophore SYBR Green I DOI

Chenchen Li,

Wei‐Chuang Kong,

Jia-Le Cui

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 11, 2024

Abstract Enrofloxacin (ENR), as a type of antimicrobial, is commonly used in animal sterilization. However, excessive ENR residues food will seriously harm human health. Therefore, this work, we developed quantitative fluorescence method for based on aptamer and SYBR Green I (SG-I). DNA aptamers with G-quadruplex space structure can be recognized inserted by SG-I to emit intense green fluorescence. In the presence ENR, preferentially bound specifically resulting change conformation, which prevented from inserting into cause quenching. The sensor quantitatively detect linear concentration range 0-600 nM, limit detection was 0.158 nM. detecting chicken, fish pork samples recovery rate 99.74–104.02%, relative standard deviation (RSD) less than 6%. aptamer-based provided safety.

Язык: Английский

Процитировано

0