International Journal of Pharmaceutics, Год журнала: 2024, Номер unknown, С. 124844 - 124844
Опубликована: Окт. 1, 2024
Язык: Английский
Small, Год журнала: 2023, Номер 20(3)
Опубликована: Сен. 7, 2023
Abstract The past two decades have witnessed a rapid progress in the development of surface charge‐reversible nanoparticles (NPs) for drug delivery and diagnosis. These NPs are able to elegantly address polycation dilemma. Converting their charge from negative/neutral positive at target site, they can substantially improve drugs diagnostic agents. By specific stimuli like shift pH redox potential, enzymes, or exogenous such as light heat, reversal NP be achieved site. activated enhances adhesion cells facilitates cellular uptake, endosomal escape, mitochondrial targeting. Because these properties, efficacy incorporated well sensitivity agents essentially enhanced. Furthermore, shown overcome biofilm formed by pathogenic bacteria shuttle antibiotics directly cell membrane microorganisms. In this review, up‐to‐date design emerging applications diagnosis highlighted.
Язык: Английский
Процитировано
35
Carbohydrate Polymers, Год журнала: 2023, Номер 305, С. 120547 - 120547
Опубликована: Янв. 5, 2023
Язык: Английский
Процитировано
20
Carbohydrate Polymers, Год журнала: 2023, Номер 316, С. 121070 - 121070
Опубликована: Май 31, 2023
This study aimed to evaluate the effect of thiolated α-cyclodextrin (α-CD-SH) on cellular uptake its payload. For this purpose, α-CD was using phosphorous pentasulfide. Thiolated characterized by FT-IR and 1H NMR spectroscopy, differential scanning calorimetry (DSC), powder X-ray diffractometry (PXRD). Cytotoxicity α-CD-SH evaluated Caco-2, HEK 293, MC3T3 cells. Dilauryl fluorescein (DLF) coumarin-6 (Cou) serving as surrogates for a pharmaceutical payload were incorporated in α-CD-SH, analyzed flow cytometry confocal microscopy. Endosomal escape investigated microscopy hemolysis assay. Results showed no cytotoxic within 3 h, while dose-dependent cytotoxicity observed 24 h. The DLF Cou up 20- 11-fold enhanced compared native α-CD, respectively. Furthermore, provided an endosomal escape. According these results, is promising carrier shuttle drugs into cytoplasm target
Язык: Английский
Процитировано
13
Drug Delivery and Translational Research, Год журнала: 2024, Номер 14(11), С. 3173 - 3185
Опубликована: Фев. 21, 2024
It was the aim of this study to design charge converting lipid nanoparticles (LNP) via a microfluidic mixing technique used for preparation and coating LNP. LNP consisting 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), cholesterol, N-(carbonyl-methoxypolyethyleneglycol-2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (MPEG-2000-DSPE), various cationic surfactants were prepared at diverging flow rate ratios (FRR) mixing. Utilizing second chip in set-up, coated with polyoxyethylene (9) nonylphenol monophosphate ester (PNPP). examined their stability different physiologically relevant media as well hemolytic cytotoxic effects. Finally, phosphate release conversion PNPP-coated evaluated after incubation alkaline phosphatase on Caco2-cells. produced an FRR 5:1 exhibited size between 80 150 nm positive zeta potential. Coating PNPP within led exhibiting negative After 1 U/ml 4 h, potential containing 1,2-dioleoyloxy-3-trimethylammonium-propane chloride (DOTAP) component shifted from - 35 mV approximately + 5 mV. other remained slightly enzymatic cleavage. Manufacturing DOTAP connection two chips instrument proves show efficient change phosphatase.
Язык: Английский
Процитировано
5
Biomacromolecules, Год журнала: 2023, Номер 24(6), С. 2587 - 2595
Опубликована: Май 24, 2023
The aim of this study was to develop peptide antibiotic-polyphosphate nanoparticles that are able overcome the enzymatic and mucus barriers providing a targeted drug release directly on intestinal epithelium. Polymyxin B-polyphosphate (PMB-PP NPs) were formed via ionic gelation between cationic anionic polyphosphate (PP). resulting NPs characterized by particle size, polydispersity index (PDI), zeta potential, cytotoxicity Caco-2 cells. protective effect these for incorporated PMB evaluated degradation studies with lipase. Moreover, diffusion investigated porcine mucus. Isolated alkaline phosphatase (IAP) employed trigger consequent release. PMB-PP exhibited an average size 197.13 ± 14.13 nm, PDI 0.36, potential -11.1 3.4 mV concentration time-dependent toxicity. They provided entire protection toward significantly (p < 0.05) higher permeating properties than PMB. When incubated isolated IAP 4 h, monophosphate constantly released from raised up -1.9 0.61 mV. According findings, promising delivery systems protect antibiotics against degradation, barrier provide at
Язык: Английский
Процитировано
10
Journal of Drug Delivery Science and Technology, Год журнала: 2023, Номер 87, С. 104789 - 104789
Опубликована: Июль 26, 2023
Язык: Английский
Процитировано
10
International Journal of Pharmaceutics, Год журнала: 2024, Номер 651, С. 123817 - 123817
Опубликована: Янв. 17, 2024
In this study, we present a novel approach for overcoming intestinal barriers by utilizing heparanase-responsive charge-converting nanocarriers (NCs). These NCs are designed to undergo charge conversion in response the activity of heparanase (HPSE), an enzyme commonly overexpressed cancer cells. Nanostructured lipid carriers (NLCs) and solid (SLNs) with positively charged core were coated heparin (Hep), resulting negative surface size between 195 220 nm. However, upon encountering heparanase, undergoes enzymatic cleavage, zeta potential shift from -22.1 +8.3 mV NLC-Hep -19.8 5.1 SLN-Hep. Heparin-coated showed more than 6-fold higher mucus permeating properties compared uncoated NCs. vitro experiments using heparanase-expressing cell line HT29 demonstrated up 4-fold improved cellular uptake co-incubation HPSE inhibitor suramin. Furthermore, was investigated on Caco-2 cells Caco-2/HT29-MTX co-culture. Overall, study highlights as promising strategy enhancing uptake.
Язык: Английский
Процитировано
3
Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер 95, С. 105540 - 105540
Опубликована: Март 6, 2024
This work aimed to investigate the influence of surface decoration orally administered lipid-based nanocarriers (LNCs) through S-protected thiolated surfactant on key properties such as mucodiffusion, adhesion and cellular uptake. comparative study with simple thiolation was performed chemically modified polyoxyethylene stearyl ether (PSE) different PEG chains exhibiting free thiol groups, which were subsequently protected by mercaptonicotinic acid or cysteine. Successively, nanostructured lipid carriers (NLCs) solid nanoparticles (SLNs) decorated PSE surfactants their impact formulations evaluated in-vitro studies intestinal mucosa. Characterization mucus diffusion first assess ability LNCs reach adsorption epithelia. Then, uptake endosomal escape analysis showed successful performance cysteine-protected thiolate surfactant-based samples in being more taken up among investigated evade from adopted endosome model. Furthermore, results confirmed that chain length determines efficacy produced systems, showing shorter polymer corresponds better Hence, considering obtained results, resulting adoption PSE10-Cys could represent interesting nanosized vehicles for drug delivery mucosal tissues.
Язык: Английский
Процитировано
3
Food Research International, Год журнала: 2023, Номер 175, С. 113631 - 113631
Опубликована: Окт. 26, 2023
Язык: Английский
Процитировано
9
Colloids and Surfaces B Biointerfaces, Год журнала: 2023, Номер 229, С. 113439 - 113439
Опубликована: Июль 5, 2023
Язык: Английский
Процитировано
8