Manufacturing of Liposomes Using a Stainless-Steel Microfluidic Device: An Investigation into Design of Experiments DOI
Ahmad Mirkani, Mohammad Reza Nabid, Sarvenaz Pakian

и другие.

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

Liposomes are highly beneficial nanocarrier systems due to their biocompatibility, low toxicity, and exceptional inclusiveness, which lead improved drug bioavailability. For biological applications, accurate control over these nanoparticles' mean size distribution is essential. Micromixers facilitate the continuous production of liposomes, enhancing precision regulation reproducibility. In this research, performance a stainless steel 316L micromixer was evaluated by using COMSOL Multiphysics simulations. The liposomes were precisely optimized design experiments techniques in microfluidic setup, then dexamethasone sodium phosphate (DSP) successfully encapsulated liposome nanoparticles. physicochemical characteristics such as ζ-potential, size, DSP loading capacity, encapsulation efficiency, release, assessed. Transmission electron microscopy dynamic light scattering analysis used examine structures liposomes. release kinetics study conducted analyze delivery system, Higuchi equation determined be most suitable equation. chip shown capable creating small-sized with small 130 nm, exhibiting monodispersed polydispersity populations.

Язык: Английский

Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4 DOI Creative Commons

Mengjiao Xue,

Yiwei Tian, Hua Zhang

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 15

Опубликована: Янв. 6, 2025

The aim of this study was to investigate the effect curcumin nanocrystals (Cur-NCs) on ferroptosis in high-glucose (HG)-induced HK-2 cells and streptozotocin (STZ)-induced diabetic nephropathy model (DN) rats. purpose is determine whether Cur NCs can become a promising treatment option for diabetes by reducing ferroptosis. Cur-NCs were prepared using microfluidic technology studied dynamic light scattering transmission electron microscopy. treated with 30 mM HG create renal tubule damage cell model. Then, viability evaluated varying concentrations (0.23, 0.47, 0.94, 1.87, 3.75, 7.5, 15, μg/mL) Cell Counting Kit-8 (CCK-8). Furthermore, vivo experiments carried out roles STZ-induced DN results showed that greatly enhanced levels LDH, MDA, Iron, lipid ROS, apoptosis, NCOA4, TFR-1, while decreasing expression GSH, GPX4, SLC7A11, FTH-1. These effects induced could be attenuated Cur-NCs. also reduced HG-induced decrease viability, as well increase ROS however erastin inhibit their effects. inhibited This demonstrates significantly attenuate recovering implying may therapy DN.

Язык: Английский

Процитировано

0

Recent Advancement of Nanocrystal Dosage Forms DOI Creative Commons
Jiayi Chen, Zhifeng Zhao,

Bhuvanesh Yathavan

и другие.

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 3, 2025

Drug nanocrystal (NC) is a formulation approach, which has been extensively exploited to enhance drug delivery for application in both dissolution rate improvement and sustained release of poorly water-soluble drugs by size reduction stabilization the particles. Due unique physicochemical pharmacokinetic properties NCs entail, versatility be incorporated into various dosage forms different disease treatments, ease scalability from laboratory development optimization setting commercial production well-developed manufacturing technologies, have attracted tremendous industrial interest, reflected increasing financial research effort over last decade. This chapter intends overview as viable approach with introduction on important aspects principles analytical characterizations. The conventional novel methods are highlighted an emphasis widely used wet milling high-pressure homogenization technologies. recent trends utilized pre-clinical studies clinical trials discussed further. In addition, currently marketed products future prospective elaborated.

Язык: Английский

Процитировано

0

Manufacturing of Liposomes Using a Stainless-Steel Microfluidic Device: An Investigation into Design of Experiments DOI
Ahmad Mirkani, Mohammad Reza Nabid, Sarvenaz Pakian

и другие.

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

Liposomes are highly beneficial nanocarrier systems due to their biocompatibility, low toxicity, and exceptional inclusiveness, which lead improved drug bioavailability. For biological applications, accurate control over these nanoparticles' mean size distribution is essential. Micromixers facilitate the continuous production of liposomes, enhancing precision regulation reproducibility. In this research, performance a stainless steel 316L micromixer was evaluated by using COMSOL Multiphysics simulations. The liposomes were precisely optimized design experiments techniques in microfluidic setup, then dexamethasone sodium phosphate (DSP) successfully encapsulated liposome nanoparticles. physicochemical characteristics such as ζ-potential, size, DSP loading capacity, encapsulation efficiency, release, assessed. Transmission electron microscopy dynamic light scattering analysis used examine structures liposomes. release kinetics study conducted analyze delivery system, Higuchi equation determined be most suitable equation. chip shown capable creating small-sized with small 130 nm, exhibiting monodispersed polydispersity populations.

Язык: Английский

Процитировано

0