Molecular Neurobiology, Год журнала: 2023, Номер unknown
Опубликована: Окт. 11, 2023
Язык: Английский
Pediatric Respirology and Critical Care Medicine, Год журнала: 2025, Номер 9(1), С. 27 - 32
Опубликована: Янв. 1, 2025
Abstract Background: Croup (laryngotracheobronchitis) is the most common pediatric disease often associated with viral infection. The incidence of croup was also high during coronavirus 2019 (COVID-19) pandemic. Materials and Methods: This retrospective cohort study based on TriNetX US Collaborative Network. Cases were patients severe acute respiratory syndrome 2 (SARS-CoV-2) infection confirmed by positive ribonucleic acid findings or other related diagnoses, whereas controls participants but no evidence COVID-19. hazard ratio (HR) incident calculated for case control groups, 95% confidence interval (95% CI) considered statistical significance. Results: Among COVID-19 group, 854 developed within 7 days after diagnosis (HR = 2.77, CI [2.44, 3.15]). higher in first 3 Compared non-COVID highest risk between 5 years age 4.37, [2.87, 6.64]). Omicron wave, followed Alpha Delta waves. from January 2023 to June 5.40, [3.52, 8.28)]). Conclusion: Our results showed that caused SARS-CoV-2 virus did not decrease due weakening virus. It because subsequent subtype. Therefore, children croup, especially those over age, should still be taken into consideration.
Язык: Английский
Процитировано
1
Micromachines, Год журнала: 2023, Номер 14(9), С. 1744 - 1744
Опубликована: Сен. 7, 2023
The pandemic of COVID-19 and its widespread transmission have made us realize the importance early, quick diagnostic tests for facilitating effective cure management. primary obstacles encountered were accurately distinguishing from other illnesses including flu, common cold, etc. While polymerase chain reaction technique is a robust determination SARS-CoV-2 in patients COVID-19, there arises high demand affordable, quick, user-friendly, precise point-of-care (POC) therapeutic settings. necessity available with rapid outcomes spurred advancement POC that are characterized by speed, automation, precision accuracy. Paper-based devices gained increasing interest recent years because rapid, low-cost detection without requiring external instruments. At present, microfluidic paper-based analysis garnered public attention accelerated development such POCT efficient multistep assays. In current review, our focus will be on fabrication modules SARS-CoV-2. Here, we included discussion various strategies viral moieties. compilation these would offer comprehensive insight into causative agent preparedness future pandemics. We also provide descriptive outline platforms, involving mechanisms, as well commercial kit their outlook.
Язык: Английский
Процитировано
19
Vaccines, Год журнала: 2024, Номер 12(5), С. 459 - 459
Опубликована: Апрель 25, 2024
Understanding the antibody response to SARS-CoV-2, virus responsible for COVID-19, is crucial comprehending disease progression and significance of vaccine therapeutic development. The emergence highly contagious variants poses a significant challenge humoral immunity, underscoring necessity grasping intricacies specific antibodies. This review emphasizes pivotal role antibodies in shaping immune responses their implications diagnosing, preventing, treating SARS-CoV-2 infection. It delves into kinetics characteristics explores current antibody-based diagnostics, discussing strengths, clinical utility, limitations. Furthermore, we underscore potential SARS-CoV-2-specific antibodies, various therapies such as monoclonal polyclonal anti-cytokines, convalescent plasma, hyperimmunoglobulin-based therapies. Moreover, offer insights vaccines, emphasizing neutralizing order confer immunity along with emerging concern (VOCs) circulating Omicron subvariants. We also highlight challenges field, risks antibody-dependent enhancement (ADE) shed light on associated original antigenic sin (OAS) effect long COVID. Overall, this intends provide valuable insights, which are advancing sensitive diagnostic tools, identifying efficient therapeutics, developing effective vaccines combat evolving threat global scale.
Язык: Английский
Процитировано
4
Vaccines, Год журнала: 2025, Номер 13(3), С. 293 - 293
Опубликована: Март 10, 2025
Background. The pathogenic coronaviruses (CoVs) MERS-CoV and SARS-CoV-2, which are responsible for the MERS outbreak COVID-19 pandemic, respectively, continue to infect humans, with significant adverse outcomes. There is a continuing need develop mucosal vaccines against these respiratory viral pathogens prevent entry replication at sites. receptor-binding domain (RBD) of CoV spike (S) protein critical vaccine target, glycan masking unique approach designing subunit improved neutralizing activity. Methods. We evaluated efficacy immunity, broad activity, cross-protection afforded by combined glycosylated encoding RBDs original SARS-CoV-2 strain (SARS2-WT-RBD), Omicron-XBB.1.5 variant (SARS2-Omi-RBD), (MERS-RBD). Results. Intranasal administration three-RBD cocktail induced effective, durable IgA systemic IgG antibodies specific S CoVs, thereby infection pseudotyped SARS-CoV-2-WT, Omicron-XBB.1.5, MERS-CoV. protected immunized mice from challenge MERS-CoV, leading reduction in titers lungs. By contrast, individual RBD proteins only such immune responses either or protecting subsequent MERS-CoV; they did not provide simultaneous protection both CoVs. Conclusions. This study describes strategy efficacious that induce cross-neutralizing highlighting potential design other pathogens.
Язык: Английский
Процитировано
0
Annals of Medicine and Surgery, Год журнала: 2023, Номер 85(12), С. 5845 - 5846
Опубликована: Окт. 4, 2023
Yadav, Shailendra; Zaman, Kamran; Bashyal, Prashant; Bhatta, Rashmi; Bhandari, Shailaj; Mohanty, Aroop; Sah, Ranjit Author Information
Процитировано
4
Опубликована: Июнь 26, 2024
Wastewater-based surveillance has emerged as an important method for monitoring SARS-CoV-2. This study investigated the presence of SARS-CoV-2 in wastewater Zambia. We conducted a longitudinal Copperbelt and Eastern provinces Zambia from October 2023 to December during which 155 samples were collected. The subjected three different concentration methods, namely bag-mediated filtration, skimmed milk flocculation, polythene glycol-based assays. Molecular detection nucleic acid was using real-time PCR. Whole genome sequencing Illumina COVIDSEQ assay. Of samples, 62 (40%) tested positive these, 13 sequences sufficient length determine lineages obtained two phylogenetically analyzed. Various Omicron subvariants detected including BA.5, XBB.1.45, BA.2.86, JN.1. Some these have been clinical cases Interestingly, phylogenetic analysis positioned sequence Province B.1.1.529 clade, suggesting that earlier variants late 2021 could still be circulating may not wholly replaced by newer subvariants. stresses need integrating into mainstream strategies circulation
Язык: Английский
Процитировано
1
Vaccine, Год журнала: 2024, Номер 42(23), С. 126203 - 126203
Опубликована: Авг. 22, 2024
Язык: Английский
Процитировано
1
Journal of Infection and Public Health, Год журнала: 2024, Номер 17(11), С. 102556 - 102556
Опубликована: Сен. 30, 2024
Язык: Английский
Процитировано
1
Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)
Опубликована: Окт. 16, 2024
Our previous study has found that monoclonal antibodies targeting a conserved epitope peptide spanning from residues 1144 to 1156 of SARS-CoV-2 spike (S) protein, namely S(1144-1156), can broadly neutralize all the prevalent strains, including wild type, Alpha, Epsilon, Delta, and Gamma variants. In study, S(1144-1156) was conjugated with bovine serum albumin (BSA) formulated Montanide ISA 51 adjuvant for inoculation in BALB/c mice its potential as vaccine candidate. Results showed titers S protein-specific IgGs neutralizing mouse sera against various variants, Omicron sublineages, were largely induced along three doses immunization. The significant release IFN-γ IL-2 also observed by ELISpot assays through stimulating vaccinated splenocytes peptide. Furthermore, vaccination S(1143-1157)- S(1142-1158)-EGFP fusion proteins elicit more than S(1144-1156)-EGFP protein. Interestingly, antisera collected inoculated exhibited better efficacy BA.2.86 JN.1 subvariants BA.1, BA.2, XBB subvariants. Since amino acid sequences are highly among immunogen containing core be designed effective COVID-19 vaccine. KEY POINTS: • Inoculation induce bnAbs stimulated splenocytes. S(1143-1157) S(1142-1158) vaccines nAbs mice.
Язык: Английский
Процитировано
1
Frontiers in Cellular and Infection Microbiology, Год журнала: 2023, Номер 13
Опубликована: Дек. 7, 2023
Ongoing SARS-CoV-2 infections are driven by the emergence of various variants, with differential propensities to escape immune containment. Single nucleotide polymorphisms (SNPs) in RNA genome result altered protein structures and when these changes occur S -gene, encoding spike protein, ability virus penetrate host cells initiate an infection can be significantly altered. As a result, vaccine efficacy prior immunity may diminished, potentially leading new waves infection. Early detection variants using rapid scalable approach will paramount for continued monitoring infections. In this study, we developed minor groove-binding (MGB) probe-based qPCR assays targeted specific SNPs which present concern (VOC), namely E484K, N501Y, G446S D405N mutations. A total 95 archived positive clinical specimens collected Johannesburg, South Africa between February 2021 March 2022 were assessed assays. To independently confirm SNP detection, Sanger sequencing relevant region -gene performed. Where PCR product could generated sequenced, 100% concordant highlighting robustness approach. These assays, described, offer opportunity easy scaling variant-defining setting.
Язык: Английский
Процитировано
2