Targeting Cysteine Protease B to Discover Antileishmanial Drugs: Directions and Advances
European Journal of Medicinal Chemistry,
Год журнала:
2025,
Номер
289, С. 117500 - 117500
Опубликована: Март 11, 2025
Язык: Английский
Detection of LPG2 and GP63 genes sequencing in patients with cutaneous leishmaniasis
Journal of Parasitic Diseases,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 8, 2025
Язык: Английский
Detection of LPG2 and GP63 Genes Sequencing in Patients with Cutaneous Leishmaniasis
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 19, 2024
Abstract
Cutaneous
Leishmaniasis
(CL)
is
a
significant
health
issue,
particularly
in
Iraq,
with
notable
increase
cases
during
the
winter
months.
This
cross-sectional
study
was
conducted
at
Ramadi
Teaching
Hospital,
selected
due
to
rising
incidence
of
CL.
A
total
10
patients,
aged
18
40,
an
average
age
29,
participated
study,
which
ran
from
December
10,
2023,
March
2024.
Blood
samples
were
collected
lesions
CL
patients
using
capillary
tubes,
ensuring
sufficient
volume
by
multiple
tubes
per
sample.
Patients
undergoing
treatment
Pentostam
as
prescribed.
The
blood
transferred
containing
sodium
chloride
solution
and
stored
-20°C
until
DNA
extraction.
After
storage,
genomic
successfully
extracted
samples.
served
template
for
polymerase
chain
reaction
(PCR)
analysis,
utilizing
specific
primers
designed
amplify
genes
associated
virulence
factors.
Among
samples,
7
tested
positive
extraction,
while
3
negative.
Of
PCR
electrophoresis
indicated
that
GP63
gene
4
Conversely,
LPG2
gene,
control
group
included
facilitate
comparison
both
genes.
Analysis
confirmed
Leishmania
major
sole
causative
agent
Anbar.
Язык: Английский
Immunomodulation by Leishmania parasites: Potential for controlling other diseases
Parasitology International,
Год журнала:
2024,
Номер
104, С. 102987 - 102987
Опубликована: Ноя. 7, 2024
In
the
mammalian
hosts,
Leishmania
parasites
survive
and
proliferate
within
phagolysosomes
of
macrophages.
To
avoid
being
killed
by
immune
cells,
utilize
their
molecules
to
manipulate
macrophages'
functions
for
survival.
Targets
such
immunomodulatory
are
not
limited
macrophages,
as
Leishmania-derived
sometimes
show
influence
on
other
cells
neutrophils,
dendritic
T
B
cells.
This
review
covers
research
immunomodulation
host
immunity
introduces
some
examples
parasite-derived
participating
in
immunomodulation.
For
example,
cell
surface
lipophosphoglycan
(LPG)
can
modulate
TLR2
signaling
PI3K/Akt
axis
macrophages
leading
induction
Th2
Because
chronic
secretion
inflammatory
cytokines
is
one
causes
immune-mediated
diseases
atherosclerosis,
Crohn's
disease,
rheumatoid
arthritis,
LPG
may
be
useful
a
drug
suppress
conditions.
The
unique
characteristics
leishmanial
pose
promise
source
drugs
controlling
than
leishmaniasis.
Язык: Английский
Protocol to culture Leishmania protozoans for lipophosphoglycan extraction and purification
STAR Protocols,
Год журнала:
2024,
Номер
5(4), С. 103468 - 103468
Опубликована: Дек. 1, 2024
Lipophosphoglycan
(LPG)
is
a
macromolecule
on
the
surface
of
Leishmania
spp.
parasites.
The
biochemical
structure
LPG
varies
throughout
parasites'
life
cycle
between
proliferative
(procyclic)
and
infective
(metacyclic)
stages,
as
well
species
strains.
Here,
we
outline
protocol
for
growing
parasites
in
vitro
to
harvest
LPG.
We
describe
steps
parasite
differentiation
extraction
purification.
has
applications
medical
research,
such
trained
immunity
immunotherapy
cancer.
For
complete
details
use
execution
this
protocol,
please
refer
dos
Santos
et
al.1.
Язык: Английский