Caspase-1/11 Controls Zika Virus Replication in Astrocytes by Regulating Glycolytic Metabolism DOI
Ingrid Sancho de Farias, Guilherme Ribeiro, Isaú H. Noronha

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Май 2, 2024

Abstract The Zika virus (ZIKV) poses a significant threat due to its association with severe neurological complications, particularly during pregnancy. Although viruses exhibit tropism for neural cells, including astrocytes, the role of these cells in controlling ZIKV replication remains unclear. In this study, we demonstrated that induces caspase-1 activation primary astrocytes despite absence classical signs inflammasome activation. Caspase-1/11−/− heightened permissiveness viral replication, accompanied by overactivation glycolytic metabolism. Inhibition glycolysis reversed susceptibility caspase-1/11−/− infection. Protein network analysis revealed mTORC as link between proteins involved and caspase-1, inhibition also suppressed replication. Furthermore, found impact caspase-1/11 on is dependent pyruvate transport mitochondria emphasizing mTORC/glycolytic pathway/pyruvate axis caspase-1/11-mediated control ZIKV. Overall, our findings elucidate caspase-1/11-dependent microbicidal mechanism providing insights into potential therapeutic targets

Язык: Английский

Fungi, immunosenescence and cancer DOI Creative Commons
Bin Xu,

Zan Luo,

Xing Niu

и другие.

Seminars in Cancer Biology, Год журнала: 2025, Номер 109, С. 67 - 82

Опубликована: Янв. 7, 2025

Язык: Английский

Процитировано

4
Fungal evasion of Drosophila immunity involves blocking the cathepsin-mediated cleavage maturation of the danger-sensing protease DOI Creative Commons
Guirong Tang, Shuangxiu Song, Junmei Shang

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(3)

Опубликована: Янв. 16, 2025

Entomopathogenic fungi play a critical role in regulating insect populations, and representative species from the Metarhizium Beauveria genera have been developed as eco-friendly biocontrol agents for managing agricultural pests. Relative to advances understanding antifungal immune responses Drosophila , knowledge of how evade defenses remains limited. In this study, we report identification characterization virulence-required effector Fkp1 robertsii . Library screening protein pull-down analysis unveiled that targets cathepsin protease CtsK1 inhibit its cleavage maturation danger-sensing serine Persephone (Psh), thereby facilitating fungal evasion defenses. The -like gene is also required bassiana infection. Transgenic expression suppressed hemolymph cysteine activity down-regulated genes. can mask Psh site without interfering with ability bait subtilisin proteases. Given evident compensatory relationship, our data indicate cascade more crucial than molecular pattern pathway defending flies against infections. This work reveals evolved distinct effectors block dual recognition pathways sheds lights on mechanisms mediating microbe–animal interactions.

Язык: Английский

Процитировано

1
Biomolecular condensates in immune cell fate DOI
Srikanth Kodali, Caroline M. Sands, Lei Guo

и другие.

Nature reviews. Immunology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

Язык: Английский

Процитировано

1
Plant secondary metabolites-mediated plant defense against bacteria and fungi pathogens DOI
Wenjuan Yang, Lu Zhang, Yong Yang

и другие.

Plant Physiology and Biochemistry, Год журнала: 2024, Номер 217, С. 109224 - 109224

Опубликована: Окт. 20, 2024

Язык: Английский

Процитировано

6
Anticancer Plant Secondary Metabolites Evicting Linker Histone H1.2 from Chromatin Activate Type I Interferon Signaling DOI Open Access
Olga Vlasova, Irina Antonova, Khamis Magomedova

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 375 - 375

Опубликована: Янв. 4, 2025

Previously we discovered that among 15 DNA-binding plant secondary metabolites (PSMs) possessing anticancer activity, 11 compounds cause depletion of the chromatin-bound linker histones H1.2 and/or H1.4. Chromatin remodeling or multiH1 knocking-down is known to promote upregulation repetitive elements, ultimately triggering an interferon (IFN) response. Herein, using HeLa cells and applying fluorescent reporter assay with flow cytometry, immunofluorescence staining quantitative RT-PCR, studied effects PSMs both evicting from chromatin not influencing their location in nucleus. We found (1) 8 PSMs, histone chromatin, activated significantly type I IFN signaling pathway out these resveratrol, berberine, genistein, delphinidin, naringenin curcumin also caused LINE1 expression. Fisetin quercetin, which induced eviction only signaling, but expression; (2) curcumin, sanguinarine kaempferol, causing significant H1.4 presence activate less intensively without any changes (3) four did eviction, displayed neither activation nor enhancement Thus, have shown for first time destabilization observed by accompanied expression often impacts this activation.

Язык: Английский

Процитировано

0
ChNLRC4, a cytoplasmic pattern recognition receptor, activates the pyroptosis signaling pathway in Mollusca DOI
Tianxiang Lin, Lu Liu, Liang Zeng

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 139632 - 139632

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Interplay of swine acute diarrhoea syndrome coronavirus and the host intrinsic and innate immunity DOI Creative Commons
Fei Zhao,

Xiao Cong,

Xiaobo Huang

и другие.

Veterinary Research, Год журнала: 2025, Номер 56(1)

Опубликована: Янв. 9, 2025

Abstract Swine acute diarrhoea syndrome coronavirus (SADS-CoV), a novel HKU2-related of bat origin, is newly emerged swine enteropathogenic that causes severe in piglets. SADS-CoV has broad cell tropism with the capability to infect wide variety cells from human and diverse animals, which implicates its ability hold high risks cross-species transmission. The intracellular antiviral immunity, comprised intrinsic innate represents first line host defence against viral infection prior onset adaptive immunity. To date, there are no vaccines drugs approved prevent or treat infection. Understanding mutual relationship between immunity crucial for development SADS-CoV. Here, we review recent advancements our understanding interplay extensive in-depth investigation on their interactive will contribute identification new targets developing intervention strategies control

Язык: Английский

Процитировано

0
Trained Decoy Nanocages Confer Selective Cuproptosis and Metabolic Reprogramming for Drug-Resistant Bacterial Targeting Therapy DOI
Wenting Zhang, Jiahao Hu,

Huiqun Hu

и другие.

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Nonantibiotic strategies are urgently needed to treat acute drug-resistant bacterial pneumonia. Recently, nanomaterial-mediated cuproptosis has arisen widespread interest due its superiority against antibiotic resistance. However, it may also cause indiscriminate and irreversible damage healthy cells. Here, we synthesized trained decoy mCuS@lm nanocages, consisting of membranes, copper sulfide, mitoquinone, luteolin for selective targeted therapeutic strategies. The nanocages could amplify through quorum sensing inhibition that cuts off interactions modulates virulence factors biofilm formation. Meanwhile, the protect cells from cuproptosis-induced mitochondrial-targeted antioxidants. Trained biomimetic membranes facilitated broad-spectrum targeting ability functioned as a neutralize cytokine storms during Moreover, reprogram metabolic conditions both bacteria host In conclusion, provide an approach challenging

Язык: Английский

Процитировано

0
IFIT3: a crucial mediator in innate immunity and tumor progression with therapeutic implications DOI Creative Commons
Rihan Wu, Hao Yang, Chunlei Liu

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 24, 2025

Interferon-Induced Protein with Tetratricopeptide Repeats 3 (IFIT3) plays a dual role in innate immunity and tumor immunity, functioning as both viral defense molecule regulator of progression. This review explores the mechanisms through which IFIT3 modulates immune responses, including interferon signaling, RIG-I-like receptors, NF-κB pathway. facilitates evasion promotes inflammation-mediated growth by regulating checkpoints microenvironment, its emerging target for cancer immunotherapy opens new avenues therapeutic strategies. Finally, this paper underscores IFIT3's potential clinical applications modulation highlighting need further research on IFIT3-targeted therapies.

Язык: Английский

Процитировано

0
Filamentation activates bacterial Avs5 antiviral protein DOI Creative Commons
Yiqun Wang, Yuqing Tian, Xu Yang

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 11, 2025

Bacterial antiviral STANDs (Avs) are evolutionarily related to the nucleotide-binding oligomerization domain (NOD)-like receptors widely distributed in immune systems across animals and plants. EfAvs5, a type 5 Avs from Escherichia fergusonii, contains an N-terminal SIR2 effector domain, NOD, C-terminal sensor conferring protection against diverse phage invasions. Despite established roles of STAND prokaryotic eukaryotic immunity, mechanism underlying their collaboration remains unclear. Here we present cryo-EM structures EfAvs5 filaments, elucidating mechanisms dimerization, filamentation, filament bundling, ATP binding, NAD+ hydrolysis, all which crucial for anti-phage defense. The NOD domains engage intra- inter-dimer interaction form individual filament, while outward contribute bundle formation. Filamentation potentially stabilizes dimeric configuration, thereby activating NADase activity EfAvs5. Furthermore, identify nucleotide kinase gp1.7 T7 as activator demonstrating its ability induce filamentation activity. Together, uncover assembly Avs5 unique switch enzyme activities perform defenses. protect Here, authors reveal that forms clustered filaments hydrolysis can be activated by protein, mechanism.

Язык: Английский

Процитировано

0