Cancer Letters, Год журнала: 2025, Номер 626, С. 217792 - 217792
Опубликована: Май 22, 2025
Язык: Английский
Journal of Experimental & Clinical Cancer Research, Год журнала: 2025, Номер 44(1)
Опубликована: Янв. 25, 2025
Abstract Background Exosomes, as extracellular membrane vesicles, play important roles in intercellular communication and can influence tumour progression. Circular RNAs (circRNAs) have been reported various malignancies are also components of exosomes. However, the role exosomal circRNAs gastric cancer (GC) progression has not completely clarified. Methods The enriched GC were identified using circRNA sequencing. biological function circMAN1A2 was investigated a series vitro vivo experiments. PKH-67 staining used to label molecular mechanism via mass spectrometry, immunoprecipitation, Western blot, single-cell RNA-sequencing data analyses. Results In our study, we determined that (hsa_circ_0000118) GC-derived Higher expression related poor survival patients (HR = 2.917, p 0.0120). Exosomal promoted suppressed antitumour activity T cells. Moreover, bound SFPQ cells cells, promoting G1/S phase transition cell cycle while inhibiting activation receptor signalling pathway decrease activity. Mechanistically, competed with FBXW11 for binding SFPQ, preventing FBXW11-mediated k48-linked ubiquitination protein degradation, thereby stabilizing expression. Conclusions Our work confirms critical immunosuppression GC. This novel axis circMAN1A2-SFPQ provides new insights into circRNA-based diagnostic therapeutic strategies.
Язык: Английский
Процитировано
3
Journal of Orthopaedic Translation, Год журнала: 2025, Номер 50, С. 44 - 55
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
1
Bioactive Materials, Год журнала: 2025, Номер 48, С. 149 - 170
Опубликована: Фев. 18, 2025
Язык: Английский
Процитировано
1
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Фев. 25, 2025
Extracellular vesicles (EVs) hold promise for tissue regeneration, but their low yield and limited therapeutic efficacy hinder clinical translation. Bioreactors provide a larger culture surface area stable environment large-scale EV production, yet ability to enhance is limited. Physical stimulation, by inducing cell differentiation modulating cargo composition, offers more efficient, cost-effective, reproducible approach compared the loading of EVs biochemical priming parental cells. Herein, effects 3D-printed perfusion bioreactor with topographical cue on macrophage bioactivity were assessed. The results indicate that increased 12.5-fold enhanced in promoting osteogenic angiogenesis via upregulated miR-210-3p. Mechanistically, fluid shear stress activates Piezo1, triggering Ca2+ influx Yes-associated protein (YAP) nuclear translocation, secretion enhancing M2 polarization conjunction morphological changes guided aligned topography. Moreover, porous electrospun membrane-hydrogel composite scaffold loaded bioreactor-derived exhibited outstanding rat cranial defect model. This study presents scalable, platform production EVs, potentially overcoming key challenges translating EV-based therapies clinic.
Язык: Английский
Процитировано
1
Small Methods, Год журнала: 2025, Номер unknown
Опубликована: Апрель 8, 2025
Abstract Exosomes, small extracellular vesicles with lipid bilayer membranes, play a crucial role in cellular communication and can transfer diverse biological cargo, including proteins, lipids, nucleic acids, from donor to recipient cells. Exosomes possess immunological properties, such as antigen delivery immune activation, along excellent drug capabilities, making them promising candidates for vaccine development. For different diseases, exosome‐based vaccines be designed therapeutic or prophylactic by leveraging immunity humoral immunity. With the emergence of precision medicine, personalized demonstrate exceptional potential. This review systematically introduces sources, biogenesis mechanisms, components exosomes describes their regulatory roles system. Subsequently, preparation, administration, therapy are discussed. Finally, applications clinical trials fields anti‐infection anti‐tumor therapies particularly highlighted, an analysis potential challenges future
Язык: Английский
Процитировано
1
Translational Stroke Research, Год журнала: 2025, Номер unknown
Опубликована: Фев. 7, 2025
Язык: Английский
Процитировано
1
Journal of Experimental & Clinical Cancer Research, Год журнала: 2025, Номер 44(1)
Опубликована: Май 2, 2025
Abstract Extracellular vesicles (EVs), particularly engineered variants, have emerged as promising tools in cancer immunotherapy due to their inherent ability modulate immune responses and deliver therapeutic agents with high specificity minimal toxicity. These nanometer-sized vesicles, which include exosomes (Exos) other subtypes, naturally participate intercellular communication are capable of carrying a diverse range bioactive molecules, including proteins, lipids, RNAs, metabolites. Recent advancements the biogenesis EVs, such strategies modify surface characteristics cargo, significantly expanded potential effective vehicles for targeted therapies. Tailoring contents incorporating immunomodulatory molecules or gene-editing (GETs), has shown outcomes enhancing anti-tumor immunity overcoming immunosuppressive tumor microenvironment (TME). Moreover, optimizing delivery mechanisms, through both passive active targeting strategies, is crucial improving clinical efficacy EV-based This review provides an overview recent developments engineering EVs immunotherapy, focusing on biogenesis, methods content customization, innovations cargo delivery. Additionally, addresses challenges associated translation therapies, issues related scalability, safety, By offering insights into current state field identifying key areas future research, this aims advance application treatment.
Язык: Английский
Процитировано
0
Advanced NanoBiomed Research, Год журнала: 2025, Номер unknown
Опубликована: Май 20, 2025
Kidney stone ranks as one of the most prevalent disorders in urology department, causing substantial personal suffering and healthcare costs globally. However, prediction, early diagnosis, treatment kidney disease are still limited. Extracellular vesicles (EVs), loaded with nucleic acids, proteins, metabolites, lipids, released by a wide variety cell types have potential biomarkers for disease. Meanwhile, some natural EVs derived from plants animals been evidenced to effects on elimination calcium oxalate crystals. More importantly, recent explorations elucidated multifaceted role therapeutic applications. These engineered can be RNAs, oligonucleotides, peptides, small molecules; this approach has shown great promise targeted drug delivery presents solution challenges prevention treatment. This review focuses blood, urine, kidney, gut microbiota, urine bacteria, which contribute crystal elimination. The is significant, offering personalized options. it crucial assess moving EV‐based therapies laboratory settings clinical
Язык: Английский
Процитировано
0
Molecular Oncology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 24, 2025
Extracellular vesicles (EVs) have been studied for several decades and are attracting growing interest among life scientists oncologists. Understanding the extent of diversity their cellular origins, structure, molecular composition, consequently functions is still under progress. EVs offer numerous diagnostic therapeutic possibilities, but many fundamental questions about need to be resolved in order effectively safely implement applications treatment human diseases.
Язык: Английский
Процитировано
0
medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Фев. 25, 2025
Summary Background Cutaneous leishmaniasis (CL) is characterized by immune dysregulation that facilitates chronic infection. Objective To investigate the role of T cells in immunopathology CL characterizing landscape skin lesions using single-cell RNA sequencing (scRNA-seq). Methods We performed scRNA-seq to profile from PBMC and patients. Results analyzed transcriptional distinct populations CD4+ CD8+ migratory lesions. resident displayed expression exhaustion markers were categorized into progenitor, transitional, terminal stages, with HAVCR2 (TIM-3) identified as a potential driver dysfunction. Compared psoriasis healthy skin, showed reduced frequency regulatory (Tregs), potentially linked oxidative stress, DNA damage, increased apoptosis. also detected double-negative (DN) γδ cells, suggesting their antigen presentation via MHC class II through TGF-β signaling. Conclusion This study provides novel insights evasion mechanisms CL, identifying TIM-3, Treg modulation, functional DN targets for future immunotherapies.
Язык: Английский
Процитировано
0