BMC Complementary Medicine and Therapies,
Год журнала:
2023,
Номер
23(1)
Опубликована: Ноя. 9, 2023
Abstract
Malaria
is
a
global
health
challenge
with
endemicity
in
sub-Saharan
Africa,
where
there
are
multiple
drug-resistant
strains
and
limited
access
to
modern
care
facilities,
especially
rural
areas.
Studies
indicate
that
African
traditional
medicine
could
make
substantial
contribution
the
reduction
of
malaria-related
deaths
achievement
universal
coverage
(UHC),
particularly
these
regions.
Thus,
this
study
evaluated
curative
antimalarial
effects
Chromolaena
odorata
leaf
extract
using
mouse
model.
Forty-five
(45)
albino
mice
weighing
between
18
22
g
were
grouped
into
nine
groups
5
animals
each.
Animals
2–9
infected
chloroquine-resistant
strain
Plasmodium
berghei,
while
3–9
subsequently
treated
10
mg/kg
chloroquine,
combination
1.4
artemether
8.75
lumefantrine
(Coartem),
varying
concentrations
fraction
from
aqueous
C.
at
day
3
post-infection.
The
findings
treatment
400
ethanolic
crude
resulted
significant
decrease
parasite
load
(97.6%),
which
was
comparable
activities
conventional
drugs
chloroquine
(98.6%)
Coartem
(98.8%).
ethyl
acetate
fractions
also
ameliorated
alterations
red
blood
cells,
white
platelets
animals.
high
activity
displayed
by
be
due
presence
quercetin
kaempferol,
as
detected
performance
liquid
chromatography
(HPLC)
analysis.
suggest
serve
an
alternative
source
malaria
therapy,
Africa.
Molecules,
Год журнала:
2022,
Номер
27(11), С. 3643 - 3643
Опубликована: Июнь 6, 2022
Arimisia
annua
L.
is
an
important
anticancer
herb
used
in
traditional
Chinese
medicine.
The
molecular
basis
underpinning
the
activity
complex
and
not
fully
understood,
but
herbal
polysaccharides,
broadly
recognised
as
having
immunomodulatory,
antioxidant
activities,
are
potential
key
active
agents.
To
examine
functions
of
polysaccharides
from
A.
annua,
their
immunomodulatory
potentials
were
evaluated,
well
structural
characterization.
water-soluble
(AAPs)
fractionated
using
size-exclusion
chromatography
to
obtain
three
dominant
fractions,
AAP-1,
AAP-2
AAP-3,
masses
centered
around
1684,
455
5.8kDa,
respectively.
isolated
evaluated
by
measuring
radical
scavenging
activities
against
DPPH●
(2,2-diphenyl-1-picrylhydrazyl
radical),
ABTS●+
(2,2′-azino-bis
(3-ethylbenzothiazoline-6-sulphonic
acid
ion),
OH●
(hydroxyl
radical).
AAP-1
displayed
high
these
radicals,
which
68%,
73%
78%,
lower
than
other
two
fractions.
Immunostimulatory
AAPs
measured
mouse
macrophages.
polysaccharide
fractions
significant
stimulated
production
tumor
necrosis
factor-α
(TNF-α)
interleukin-6
(IL-6).
showed
immunostimulatory
(16-fold
increase
IL-6
compared
control
13-fold
TNF-α)
with
low
toxicity
(>60%
cell
viability
at
125
μg/mL
concentration).
Preliminary
characterization
was
carried
out
gas
(GC)
FTIR
techniques.
results
indicate
that
pyranose-containing
β-linkages,
AAP-3
a
β-fructofuranoside.
suggest
candidates
for
immunotherapy
cancer
treatment.
ACS Omega,
Год журнала:
2023,
Номер
8(47), С. 45078 - 45087
Опубликована: Ноя. 14, 2023
Here,
we
propose
the
molecular
hybridization
of
dihydroartemisinin
(DHA)
and
ursodeoxycholic
bile
acid
(UDCA),
approved
drugs,
for
preparation
antiviral
agents
against
SARS-CoV-2.
DHA
UDCA
were
selected
on
basis
their
recently
demonstrated
in
vitro
activity
A
selection
DHA-UDCA-based
hybrids
obtained
by
varying
nature
linkage
conjugation
point
as
well
unconjugated
tested
cytotoxicity
anti-SARS-CoV-2
Vero
E6
Calu-3
human
lung
cells.
The
hybrid
DHA-t-UDCMe,
via
click
chemistry
a
gram
scale,
was
identified
potential
candidate
SARS-CoV-2
infection
treatment
due
to
significant
reduction
viral
replication,
possibly
involving
ACE2
downregulation,
no
cytotoxicity,
chemical
stability.
Applied Biological Chemistry,
Год журнала:
2024,
Номер
67(1)
Опубликована: Март 21, 2024
Abstract
Artemisia
species
have
significant
commercial,
medical,
and
economic
value
are
widely
used
in
the
traditional
medicine
pharmaceutical
industries.
Artemisinin,
a
powerful
antimalarial
agent,
is
an
important
metabolite
that
primarily
accumulates
within
glandular
trichomes
(GTs)
on
leaf
surface
of
plants.
Trichomes
arising
from
elongation
epidermal
cells
can
be
classified
into
GTs
non-glandular
(NGTs)
based
their
morphology.
NGTs
present
species,
relationship
between
artemisinin
has
been
extensively
studied;
however,
correlation
remains
relatively
unexplored.
In
this
study,
we
inferred
derivatives
trichome
characteristics
type
developmental
stage,
age
conducted
analyses
to
investigate
factors
influencing
content
across
different
species.
Artemisinin
its
exhibited
variations
distribution
age,
with
decreasing
trend
observed
most
as
stage
progressed.
Noticeable
differences
among
were
shape,
morphology,
distribution.
Although
data
did
not
evidently
differentiate
groups,
principal
component
analysis
revealed
was
positively
associated
density,
indicating
coefficient
0.56
(
p
<
0.0001).
Therefore,
number
may
affect
Plants,
Год журнала:
2024,
Номер
13(7), С. 1050 - 1050
Опубликована: Апрель 8, 2024
In
order
to
determine
the
distribution
area
and
amount
of
Artemisia
annua
Linn.
(A.
annua)
in
China,
this
study
estimated
current
A.
specimens
based
on
field
survey
sample
data
obtained
from
Fourth
National
Census
Chinese
Medicinal
Resources.
The
was
calculated
using
maximum
entropy
model
(MaxEnt
model)
spatio-temporal
kriging
interpolation.
influencing
factors
affecting
spatial
variations
were
studied
geographic
probes.
results
indicated
that
China
about
700
billion
2019.
mainly
distributed
circular
coastal
belt
Shandong
Peninsula,
central
Hebei,
Tianjin,
western
Liaoning,
along
Yangtze
River
middle
lower
reaches
Jiangsu,
Anhui,
northern
Chongqing
provinces.
main
are
precipitation
wettest
warmest
seasons,
average
annual
precipitation,
temperature
coldest
driest
seasons.
show
is
strongly
influenced
by
temperature.
Biotechnology and Applied Biochemistry,
Год журнала:
2023,
Номер
70(6), С. 1870 - 1880
Опубликована: Июль 9, 2023
Artemisinin
is
the
most
practical
medication
for
treatment
of
malaria,
but
only
very
minimally
synthesized
in
Artemisia
annua,
significantly
less
than
market
needs.
In
this
study,
indole-3-acetic
acid
(IAA)
was
used
to
investigate
its
effects
on
trichomes,
artemisinin
accumulation,
and
biosynthetic
gene
expression
A.
anuua.
The
results
showed
that
exogenous
IAA
could
contribute
growth
development
annua
increase
density
trichomes.
Analysis
using
liquid
chromatography-tandem
mass
spectrometry
(LC-MS/MS)
indicated
dihydroartemisinic
(DHAA)
contents
were
increased
by
1.9-fold
(1.1
mg/g)
2.1-fold
(0.51
after
comparison
with
control
lines
(CK),
respectively.
Furthermore,
quantitative
real-time
PCR
AaADS,
AaCYP71AV1,
AaALDH1,
AaDBR2,
four
critical
enzyme
genes
biosynthesis
artemisinin,
had
relatively
high
transcription
levels
leaves
treated
IAA.
summary,
study
a
feasible
strategy
enhance
production,
which
paves
way
further
metabolic
engineering
biosynthesis.
Chem & Bio Engineering,
Год журнала:
2024,
Номер
1(1), С. 44 - 52
Опубликована: Янв. 4, 2024
Artemisinin,
a
drug
used
to
treat
malaria,
can
be
chemically
synthesized
or
extracted
from
Artemisia
annua
L.
However,
the
extraction
method
for
artemisinin
biomass
needs
more
sustainable
while
maintaining
enhancing
its
bioactivity.
This
work
investigates
use
of
aqueous
solutions
salts
and
ionic
liquids
with
hydrotropic
properties
as
alternative
solvents
Among
investigated
solvents,
cholinium
salicylate
sodium
were
found
most
promising.
To
optimize
process,
response
surface
was
further
applied,
in
which
time,
hydrotrope
concentration,
temperature
optimized.
The
optimized
conditions
resulted
yields
up
6.50
6.44
mg·g–1,
obtained
salicylate,
respectively.
extracts
tested
their
antimalarial
activity,
showing
higher
efficacy
against
Plasmodium
falciparum
strain
compared
pure
(synthetic)
conventional
organic
solvents.
Characterization
revealed
presence
together
other
compounds,
such
artemitin,
chrysosplenol
D,
arteannuin
B,
J.
These
compounds
act
synergistically
enhance
activity
extracts.
Given
growing
concern
about
resistance,
results
here
pave
way
development
biobased
drugs.
