Influence of Modification of Cysteine Residues in the Carbohydrate-Binding Domain on Cellulase Activity: Achieving Enhanced Performance and pH-Sensitive Recovery DOI
Huihui Zhang, Zhongliang Su, Yuyang Sun

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

The conversion of cellulosic biomass into fermentable sugars represents a pivotal stage in the environmentally friendly and sustainable production clean fuels chemicals. In this paper, new strategy to enhance enzyme performance was proposed. This can achieve pH-sensitive recovery cellulase while improving catalytic activity stability enzyme. achieved by means chemical modification cysteine residue (−SH) carbohydrate-binding domain (CBD) with PEG-based modifier (Mal-PEG-FA). It shown that altered conformation cellulase, which significantly improved efficiency CBD enzyme, turn promoted particular, modified molecular weight 10 k demonstrated highest level activity, an increase 130.02% comparison native cellulase. Ultimately, effective aqueous phase system accomplished adjusting pH system. After five cycles, still maintained 81.0% its initial enzymatic activity. research is significant strategic importance for promotion efficient utilization.

Язык: Английский

Influence of Modification of Cysteine Residues in the Carbohydrate-Binding Domain on Cellulase Activity: Achieving Enhanced Performance and pH-Sensitive Recovery DOI
Huihui Zhang, Zhongliang Su, Yuyang Sun

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

The conversion of cellulosic biomass into fermentable sugars represents a pivotal stage in the environmentally friendly and sustainable production clean fuels chemicals. In this paper, new strategy to enhance enzyme performance was proposed. This can achieve pH-sensitive recovery cellulase while improving catalytic activity stability enzyme. achieved by means chemical modification cysteine residue (−SH) carbohydrate-binding domain (CBD) with PEG-based modifier (Mal-PEG-FA). It shown that altered conformation cellulase, which significantly improved efficiency CBD enzyme, turn promoted particular, modified molecular weight 10 k demonstrated highest level activity, an increase 130.02% comparison native cellulase. Ultimately, effective aqueous phase system accomplished adjusting pH system. After five cycles, still maintained 81.0% its initial enzymatic activity. research is significant strategic importance for promotion efficient utilization.

Язык: Английский

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