Chemical Composition of Taraxacum Officinale Extracts and Anti-Proliferative Effect on A549 and HT-29 Cell Lines Through Regulating the Expression of Apoptosis-Related Genes DOI Open Access
Serkan Kapancık

Cumhuriyet Science Journal, Год журнала: 2024, Номер 45(4), С. 693 - 700

Опубликована: Дек. 30, 2024

The use of medicinal plants is still great importance as an alternative treatment method in neurodegenerative diseases, cardiovascular patients and cancer. Taraxacum officinale plant one the medical used many diseases diuretic, hypolipidemic antidiabetic thanks to active components it contains. In order examine effect officinal on proliferation apoptosis cancer, our study, we determined chemical composition by gas chromatography-mass spectrometry (GC-MS) its antioxidant status using a commercial kit. Additionally, anti-cancer water, methanol chloroform extracts A549 HT-29 cancer cell lines with help MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) method. Finally, aimed determine relationship between extract plant, which has highest properties, determining expressions BAX, BCL-2 Caspase-3, are among genes associated apoptosis, lines, Real-time Polymerase Chain Reaction (RT-PCR) analysis. We that increasing caspase-3 lung BAX decreasing expression colon lines. light data obtained, can say properties cause decrease regulating related

Язык: Английский

Characterization and investigation of Bio-inspired ZnO nanoparticles using Prosopis juliflora and Cordia dichotoma for antioxidant and antibacterial applications DOI Creative Commons

R. Subbiah,

S. Anitha,

R. Premila

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Abstract Green synthesis of zinc oxide nanoparticles was carried out using Prosopis juliflora bark extract and Cordia dichotoma leaf with acetate precursor in the presence 2 M NaOH solution. The prepared were characterized by Powder X-ray diffraction (PXRD), UV-visible absorption spectroscopy, Fourier transform infrared spectroscopy (FTIR), Photoluminescence (PL) to study its structural optical properties. studies confirmed hexagonal wurtzite phase samples an average crystallite size calculated Scherrer equation 38.22 nm 29.95 for mediated (PJZ) (CDZ) NPs respectively. Williamson-Hall modified form uniform deformation model (UDM), stress (USDM) energy-density (UDEDM) determine micro-strain ZnO nanomaterials. Tauc’s plot method revealed energy gap value 2.86 eV PJZ sample 2.95 CDZ due quantum confinement effect. FTIR spectra recorded ascertain biomolecules involved green synthesis. PL showed a strong UV emission peak around 370 visible broad band at 554 which is singly ionized oxygen vacancies. An effective prepare nanomaterials efficient antioxidant potential reported this study. current has also demonstrated that small particle responsible significant antibacterial activity against both gram-positive gram-negative bacteria.

Язык: Английский

Процитировано

0

Biogenic synthesis of zinc oxide nanoparticles mediated by the extract of Simarouba glauca leaf and its multifaceted applications DOI

Biswaprakash Sarangi,

Shashikanta Behera,

Rajesh Kumar Meher

и другие.

Inorganica Chimica Acta, Год журнала: 2025, Номер unknown, С. 122706 - 122706

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Chemical Composition of Taraxacum Officinale Extracts and Anti-Proliferative Effect on A549 and HT-29 Cell Lines Through Regulating the Expression of Apoptosis-Related Genes DOI Open Access
Serkan Kapancık

Cumhuriyet Science Journal, Год журнала: 2024, Номер 45(4), С. 693 - 700

Опубликована: Дек. 30, 2024

The use of medicinal plants is still great importance as an alternative treatment method in neurodegenerative diseases, cardiovascular patients and cancer. Taraxacum officinale plant one the medical used many diseases diuretic, hypolipidemic antidiabetic thanks to active components it contains. In order examine effect officinal on proliferation apoptosis cancer, our study, we determined chemical composition by gas chromatography-mass spectrometry (GC-MS) its antioxidant status using a commercial kit. Additionally, anti-cancer water, methanol chloroform extracts A549 HT-29 cancer cell lines with help MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) method. Finally, aimed determine relationship between extract plant, which has highest properties, determining expressions BAX, BCL-2 Caspase-3, are among genes associated apoptosis, lines, Real-time Polymerase Chain Reaction (RT-PCR) analysis. We that increasing caspase-3 lung BAX decreasing expression colon lines. light data obtained, can say properties cause decrease regulating related

Язык: Английский

Процитировано

0