Isolation and Probiotic Characterization of Lactic Acid Bacteria from the Phylloplanes of Edible and Culinary Leaves DOI Creative Commons

S Shashank,

Bablu Kumar,

V. C. Suvarna

и другие.

Опубликована: Апрель 18, 2025

Abstract The phylloplane of edible and culinary plants harbours diverse microbial communities, including lactic acid bacteria (LAB), which remain underexplored for their probiotic potential. This study aimed to isolate LAB from the leaf surfaces eight plant species: Sauropus androgynus, Piper betle, Centella asiatica, Artocarpus heterophyllus, Pandanus amaryllifolius, Musa paradisiaca, Curcuma longa , Ricinus communis . isolates were characterized essential traits, tolerance acid, bile salts, NaCl, phenol, temperature resistance, haemolytic activity, antibiotic susceptibility, auto-aggregation, cell surface hydrophobicity. All exhibited γ-haemolytic confirming non-pathogenic nature. Notably, Lactobacillus acidophilus LAB-10 displayed superior gastrointestinal stress tolerance, high hydrophobicity (72% 60%, respectively), strong auto-aggregation (42% 36%). These strains tolerated NaCl concentrations up 6%, phenol (0.4%), grew optimally at 37–40□°C pH 2.0–3.5. Antibiotic susceptibility varied, with some resistant multiple antibiotics. highlights as a valuable source functional attributes suitable potential applications in foods therapeutic formulations.

Язык: Английский

Isolation and Probiotic Characterization of Lactic Acid Bacteria from the Phylloplanes of Edible and Culinary Leaves DOI Creative Commons

S Shashank,

Bablu Kumar,

V. C. Suvarna

и другие.

Опубликована: Апрель 18, 2025

Abstract The phylloplane of edible and culinary plants harbours diverse microbial communities, including lactic acid bacteria (LAB), which remain underexplored for their probiotic potential. This study aimed to isolate LAB from the leaf surfaces eight plant species: Sauropus androgynus, Piper betle, Centella asiatica, Artocarpus heterophyllus, Pandanus amaryllifolius, Musa paradisiaca, Curcuma longa , Ricinus communis . isolates were characterized essential traits, tolerance acid, bile salts, NaCl, phenol, temperature resistance, haemolytic activity, antibiotic susceptibility, auto-aggregation, cell surface hydrophobicity. All exhibited γ-haemolytic confirming non-pathogenic nature. Notably, Lactobacillus acidophilus LAB-10 displayed superior gastrointestinal stress tolerance, high hydrophobicity (72% 60%, respectively), strong auto-aggregation (42% 36%). These strains tolerated NaCl concentrations up 6%, phenol (0.4%), grew optimally at 37–40□°C pH 2.0–3.5. Antibiotic susceptibility varied, with some resistant multiple antibiotics. highlights as a valuable source functional attributes suitable potential applications in foods therapeutic formulations.

Язык: Английский

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