Sivelestat sodium alleviated sepsis-induced acute lung injury by inhibiting TGF-β/Smad signaling pathways through upregulating microRNA-744-5p

Journal of Thoracic Disease, Год журнала: 2024, Номер 16(10), С. 6616 - 6633

Опубликована: Окт. 1, 2024

Acute lung injury (ALI) is one of the most common critical illnesses in clinical practice, with sepsis being cause ALI. Sivelestat sodium (SV) hydrate a highly effective inhibitor neutrophil elastase, specifically targeting ALI related to systemic inflammatory response syndrome. The aim this study examine mechanisms by which SV can reduce severity resulting from sepsis.

Язык: Английский

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Astragalus regulates the intestinal immune response during sepsis by mediating ILC3 proliferation through RORγt

Heliyon, Год журнала: 2023, Номер 9(7), С. e17766 - e17766

Опубликована: Июнь 28, 2023

BackgroundSepsis is a common complication of many diseases and associated with high morbidity mortality rates. Astragalus can improve humoral innate immunity, inhibit inflammatory responses, protect immune cells organs from damage. However, to the best our knowledge there are no reports on whether astragalus regulate intestinal function during sepsis.MethodsIn this study, rat cecal ligation puncture model sepsis was used investigate effects treatment, following which apoptosis rate lymphocytes Peyer's patches (PP) determined. Type 3 lymphoid (ILC3) were cultured in vitro further evaluate mechanisms astragalus.ResultsThe level PP rats significantly increased, number ILC3 reduced, compared sham operation group, aggravated injury ultimately led death rats. treatment inhibited PP, increased ILC3, improved environment group. RT-PCR revealed that retinoic acid-related orphan receptor γt (RORγt) agonist LYC-55716 both promote expression interleukin (IL)-17A, IL-17F, IL-22, interferon-γ, granulocyte-macrophage colony-stimulating factor mRNA. Mechanistically, promotes proliferation through RORγt, thereby reducing damage.ConclusionAstragalus, via generation improves sepsis.

Язык: Английский

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Calycosin Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Mice through the miR-375-3p /ROCK2 Axis

Journal of Investigative Surgery, Год журнала: 2023, Номер 36(1)

Опубликована: Июль 3, 2023

Objective: Septic patients are especially vulnerable to acute lung injury (ALI). Calycosin (CAL) has various promising pharmacological activities. This paper aims expound on the role of CAL in mice with sepsis-induced ALI and associated mechanisms.Methods: Mouse models were established using lipopolysaccharide (LPS). Pulmonary histopathological changes observed by HE staining. Cell apoptosis was assessed TUNEL edema evaluated measuring wet/dry weight. Bronchoalveolar lavage fluid (BALF) collected count inflammatory cells. In vitro LPS MLE-12 miR-375-3p expression determined RT-qPCR. viability MTT assay flow cytometry. Levels cytokines ELISA. The target relationship between ROCK2 analyzed dual-luciferase assay. protein level Western blot.Results:miR-375-3p weakly-expressed ALI, treatment elevated expression. mitigated pulmonary tissue damage edema, decreased cells, downregulated levels pro-inflammatory cytokines, upregulated anti-inflammatory ALI. increased cell inflammation Inhibition partially abrogated CAL-mediated protective action attenuated LPS-induced targeting ROCK2.Conclusion: upregulates ROCK2, thus protecting against mice.

Язык: Английский

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Pharmacological effects of Bufei Jianpi granule on chronic obstructive pulmonary disease and its metabolism in rats

Frontiers in Pharmacology, Год журнала: 2022, Номер 13

Опубликована: Дек. 15, 2022

This work was performed to determine the pharmacological effects of Bufei Jianpi granules on chronic obstructive pulmonary disease and its metabolism in rats. Chronic (COPD), ranked as third leading cause death worldwide, is seriously endangering human health. At present, pathogenesis COPD complex unclear, drug treatment mainly aims alleviate improve symptoms; however, they cannot achieve purpose eradicating disease. granule (BJG) a Chinese medicine developed by First Affiliated Hospital Henan University Traditional Medicine for treating COPD. study focuses BJG rats, aiming provide scientific basis developing against A total 72 Sprague–Dawley (SD) rats were divided into blank group, model positive control groups (2.36, 1.18, 0.59 g/kg). Except other administered lipopolysaccharide (LPS) combined with smoking 6 weeks establish model. After another treatment, therapeutic effect evaluated. In (2.36 g/kg) cough condition significantly relieved body weight close that group. Compared mortality 16.7% no deaths occurred (1.18 groups. The lung tissue damage less than MV, PIF, PEF, EF50 observably increased dose-dependent manner, while sRaw, Raw, FRC obviously decreased. Also, contents IL-6, IL-8, TNF-α, PGE2, MMP-9, NO serum BALF lowered dramatically all All indicators present an obvious dose–effect relationship. On this basis, UPLC-QTOF-MS/MS technology used analyze characteristic metabolites under physiological pathological conditions. 17 prototype 7 metabolite components detected, concentration most pathologic state. It suggested has absorption chemical exhibited significant differences

Язык: Английский

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Lipopolysaccharide-induced lung cell inflammation and apoptosis are enhanced by circ_0003420/miR-424-5p/TLR4 axis via inactivating the NF-κB signaling pathway

Transplant Immunology, Год журнала: 2022, Номер 74, С. 101639 - 101639

Опубликована: Июнь 3, 2022

Язык: Английский

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