Tetramethylpyrazine Confers Protection Against Oxidative Stress and NLRP3-Dependent Pyroptosis in Rats with Endometriosis

Organogenesis, Год журнала: 2025, Номер 21(1)

Опубликована: Фев. 18, 2025

Tetramethylpyrazine (TMP) has been confirmed to suppress inflammation in endometriosis (EMs). Herein, this study investigated whether and how TMP affected NLRP3 inflammasomes oxidative stress EMs. After establishment of an EMs rat model, rats were treated with different concentrations TMP. The size endometriotic lesions the latency frequency torsion recorded, followed by measurement relevant indicators (TNF-α, IL-6, IL-2, IL-10, MDA, SOD, GSH, CAT, ROS, NLRP3, ASC, GSDMD, caspase-1, Nrf2, HO-1). experimentally determined that treatment markedly decreased improved levels inflammatory proteins, markers, inflammasome, pyroptotic proteins elevated EMs, all which reversed upon treatment. Additionally, activities CAT lowered partly abrogated Furthermore, downregulation Nrf2 HO-1 was counteracted To sum up, represses excessive stress, inflammasome activation, pyroptosis may activate Nrf2/HO-1 pathway.

Язык: Английский

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Mitochondrial Dynamics and Metabolism in Macrophages for Cardiovascular Disease: a review

Phytomedicine, Год журнала: 2025, Номер 140, С. 156620 - 156620

Опубликована: Март 7, 2025

Язык: Английский

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Pyroptosis in septic lung injury: Interactions with other types of cell death

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 169, С. 115914 - 115914

Опубликована: Ноя. 24, 2023

Sepsis is a life-threatening systemic inflammatory response syndrome caused by the host imbalanced to infection. Lung injury most common complication of sepsis and one leading causes patient death. Pyroptosis specific programmed cell death characterized release cytokines. Appropriate pyroptosis can reduce tissue damage exert protective effect against infection during sepsis. However, overactivated results in massive death, septic shock, multiple organ dysfunction syndrome, even an increased risk secondary Recent studies suggest that interact with cross-regulate other types programs establish complex network which participates occurrence development lung injury. This review will focus on interactions between including apoptosis, necroptosis, PANoptosis, NETosis, autophagy, ferroptosis, summarize role sepsis-induced injury, discuss potential therapeutic strategies targeting treatment.

Язык: Английский

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Hydroxysafflor yellow A protects against colitis in mice by suppressing pyroptosis via inhibiting HK1/NLRP3/GSDMD and modulating gut microbiota

Toxicology and Applied Pharmacology, Год журнала: 2023, Номер 467, С. 116494 - 116494

Опубликована: Март 29, 2023

Язык: Английский

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Mechanism of Action and Therapeutic Implications of Nrf2/HO-1 in Inflammatory Bowel Disease

Antioxidants, Год журнала: 2024, Номер 13(8), С. 1012 - 1012

Опубликована: Авг. 20, 2024

Oxidative stress (OS) is a key factor in the generation of various pathophysiological conditions. Nuclear erythroid 2 (NF-E2)-related (Nrf2) major transcriptional regulator antioxidant reactions. Heme oxygenase-1 (HO-1), gene regulated by Nrf2, one most critical cytoprotective molecules. In recent years, Nrf2/HO-1 has received widespread attention as regulatory pathway for intracellular defense against oxidative stress. It considered potential target treatment inflammatory bowel disease (IBD). This review highlights mechanism action and therapeutic significance IBD complications (intestinal fibrosis colorectal cancer (CRC)), well phytochemicals targeting IBD. The results suggest that effects on mainly involve following aspects: (1) Controlling to reduce intestinal inflammation injury; (2) Regulation flora repair mucosal barrier; (3) Prevention ferroptosis epithelial cells. However, due complex role Nrf2/HO-1, more nuanced understanding exact mechanisms involved way forward future.

Язык: Английский

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Melatonin alleviates septic ARDS by inhibiting NCOA4-mediated ferritinophagy in alveolar macrophages

Cell Death Discovery, Год журнала: 2024, Номер 10(1)

Опубликована: Май 24, 2024

Abstract Ferroptosis is a novel form of programmed cell death which can exacerbate lung injury in septic acute respiratory distress syndrome (ARDS). Alveolar macrophages, crucial innate immune cells, play pivotal role the pathogenesis ARDS. Ferritinophagy process ferritin degradation mediated by nuclear receptor coactivator 4 (NCOA4) releases large amounts iron ions thus promoting ferroptosis. Recent evidence revealed that inhibiting macrophage ferroptosis effectively attenuate pulmonary inflammatory injury. Melatonin (MT), an endogenous neurohormone, has antioxidant and anti-inflammatory effects reduce However, it not clear whether MT’s protective effect related to inhibition ferritinophagy. Our vitro experiments demonstrated MT decreased intracellular malondialdehyde (MDA), Fe 2+ , lipid peroxidation levels, increased glutathione (GSH) levels proliferation, upregulated peroxidase (GPX4) heavy chain 1 (FTH1) protein LPS-treated macrophages. Mechanistically, antiferroptotic on macrophages was significantly compromised overexpression NCOA4. vivo alleviated expression NCOA4 FTH1 alveolar mice. Furthermore, improved mitigated damage tissue, ultimately increasing survival rates These findings indicate inhibit NCOA4-mediated ferritinophagy manner, thereby ameliorating

Язык: Английский

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Acute Lung Injury and the NLRP3 Inflammasome

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 3801 - 3813

Опубликована: Июнь 1, 2024

Abstract: Acute lung injury (ALI) manifests through harm to the capillary endothelium and alveolar epithelial cells, arising from a multitude of factors, leading scattered interstitial alterations, pulmonary edema, subsequent acute hypoxic respiratory insufficiency. (ALI), along with its more serious counterpart, distress syndrome (ARDS), carry fatality rate that hovers around 30– 40%. Its principal pathological characteristic lies in unchecked inflammatory reaction. Currently, main strategies for treating ALI are alleviation inflammation prevention failure. Concerning etiology ALI, NLRP3 Inflammasome is essential body's innate immune response. The composition this inflammasome complex includes NLRP3, pyroptosis mediator ASC, pro-caspase-1. Recent research has reported response centered on inflammasomes plays key part may hence be prospective candidate therapeutic intervention. In review, we present an overview ailment characteristics constitution operation within framework. We also explore targeting combat injury. Keywords: injury, inflammasome, caspase-1, IL-1β, IL-18

Язык: Английский

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Kirenol inhibits inflammation challenged by lipopolysaccharide through the AMPK-mTOR-ULK1 autophagy pathway

International Immunopharmacology, Год журнала: 2023, Номер 116, С. 109734 - 109734

Опубликована: Янв. 25, 2023

Kirenol is a bioactive substance isolated from Herba Siegesbeckiae. Although the anti-inflammatory activity of kirenol has been well documented, its role in autophagy remains unknown. The present study aimed to investigate protective on inflammation challenged by lipopolysaccharide (LPS) acute lung injury (ALI) cell and mouse models unravel underlying mechanisms, with particular focus autophagy. For this purpose, an ALI were established, effects expression molecules related examined. results revealed that could significantly inhibit inflammatory cytokines secretion cells mice injured LPS; effect may be attributed enhanced as evidenced up-regulation LC3-II down-regulation p62 both vitro vivo. Phosphorylated AMPK ULK1 increased, while phosphorylated mTOR decreased kirenol-treated model. Moreover, inhibition using inhibitor or 3-MA chloroquine (CQ) reversed autophagy-enhancement exposure vitro, indicating enhance activating AMPK-mTOR-ULK1 pathway. RNA sequencing suggested was strongly biological functions response signaling Further vivo model studies demonstrated against inflammation, such improved histopathology, edema, leukocyte infiltration abolished 3-MA. These findings implicate can LPS-induced via

Язык: Английский

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Saikosaponin A and Its Epimers Alleviate LPS-Induced Acute Lung Injury in Mice

Molecules, Год журнала: 2023, Номер 28(3), С. 967 - 967

Опубликована: Янв. 18, 2023

The purpose of this work was to illustrate the effect processing with vinegar on saikosaponins Bupleurum chinense DC. (BC) and protective effects saikosaponin A (SSA), b1 (SSb1), b2 (SSb2), D (SSD) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) mice. We comprehensively evaluated anti-inflammatory potential mechanisms SSA, SSb1, SSb2, SSD through an LPS-induced ALI model using intratracheal injection. results showed that significantly decreased pulmonary edema; reduced levels IL-6, TNF-α, IL-1β serum tissues; alleviated pathological damage; genes expression NF-κB/TLR4-related proteins. Interestingly, they were similar structure, but SSb2 had a better at same dose, according principal component analysis. These findings indicated it may not have been comprehensive only use SSA as indicators evaluate quality BC, especially contents SSb1 vinegar-processed BC increased.

Язык: Английский

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Role of mitochondrial stress and the NLRP3 inflammasome in lung diseases

Inflammation Research, Год журнала: 2023, Номер 72(4), С. 829 - 846

Опубликована: Март 11, 2023

Язык: Английский

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