Moscow University Biological Sciences Bulletin, Год журнала: 2023, Номер 78(2), С. 45 - 52
Опубликована: Июнь 1, 2023
Язык: Английский
International Immunopharmacology, Год журнала: 2024, Номер 142, С. 113051 - 113051
Опубликована: Сен. 4, 2024
Язык: Английский
Процитировано
1
Moscow University Biological Sciences Bulletin, Год журнала: 2023, Номер 78(1), С. 8 - 13
Опубликована: Март 1, 2023
Язык: Английский
Процитировано
3
Vestnik Moskovskogo universiteta Seria 16 Biologia, Год журнала: 2023, Номер 78(№2, 2023), С. 55 - 63
Опубликована: Июль 12, 2023
Neutrophils are the most abundant leukocytes in blood and “ rst line” of defense against pathogens ammation foci, where they perform e ector functions such as phagocytosis, degranulation, generation reactive oxygen species, formation neutrophil extracellular traps. For a long time, it was believed that neutrophils short-lived terminally di erentiated phagocytes. However, this point view has been changed after found these cells able to interact with other populations leukocytes, well mediate relationship between innate adaptive immunity. In recent years, lot data accumulated indicating ability acquire function antigen-presenting number pathological ammatory conditions. addition, can express major histocompatibility complex class II costimulatory molecules under uence speci c cytokines vitro system activate T lymphocytes. This review summarizes current on neutrophils, proposed mechanisms regulation process its signi cance normal
Язык: Английский
Процитировано
2
Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 166, С. 115400 - 115400
Опубликована: Авг. 31, 2023
The pleiotropic effects of high-density lipoprotein (HDL), including its protective properties against sepsis, are attributed to the sphingosine 1-phosphate and apolipoprotein M (ApoM) that carried on lipoproteins. In this study, we attempted elucidate possible mechanisms underlying sepsis coagulopathic state by considering modulation NETosis. Our results revealed in a lipopolysaccharide-induced mouse model, levels NETosis markers, such as plasma DNA histone, were elevated ApoM-knockout (KO) mice attenuated ApoM-overexpressing mice. ApoM-KO mice, survival rate decreased occurrence rates coagulopathy organ injury increased following administration histone. Treatment with conditioned medium cells observed HL-60S differentiated into neutrophils inhibited through suppression S1P1 or S1P4. attenuation PKCδ PKCα/β S1P4 activation may also be involved. injuries an injection histone; these partially S1P1, 3, S1P4, antagonists. Furthermore, exogenous ApoM protected challenged histone from developing conclusion, ApoM/S1P axis protects PKC development drugs targeting beneficial for treatment pathological conditions involving uncontrolled NETosis, sepsis.
Язык: Английский
Процитировано
2
International Immunopharmacology, Год журнала: 2023, Номер 127, С. 111366 - 111366
Опубликована: Дек. 20, 2023
Язык: Английский
Процитировано
1
Vestnik Moskovskogo universiteta Seria 16 Biologia, Год журнала: 2024, Номер 78(№4, 2023), С. 243 - 249
Опубликована: Март 8, 2024
Neutrophils release decondensed nuclear chromatin or Neutrophil Extracellular Traps (NETs) in response to a great number of physiological stimuli protect the host from pathogens. However, NETs have recently been shown play an important role pathogenesis autoimmune, infl ammatory, and malignant diseases. Therefore, understanding molecular mechanisms underlying formation, usually leading neutrophil death (NETosis), is extremely control aberrant chromatin. Mitogen-activated protein kinases (MAP-kinases) are involved various cellular functions such as oxidative burst, chemotaxis, degranulation, adhesion, apoptosis, but their NETosis not well understood. Three families MAP-kinases, p38, ERK1/2, JNK, described human neutrophils, we investigated contribution kinase B Akt1/2 burst using inhibitory analysis. We that MAPkinase p38 activated upon stimulation with calcium ionophore ionomycin. these induced by diacylglycerol mimetic phorbol 12-myristate 13-acetate (PMA) PMA-induced NETosis.
Язык: Английский
Процитировано
0
Vestnik Moskovskogo universiteta Seria 16 Biologia, Год журнала: 2024, Номер 79(№1, 2024), С. 66 - 71
Опубликована: Июнь 3, 2024
Neutrophil extracellular traps (NETs) are decondensed nuclear chromatin, decorated with bactericidal proteins of various cell organelles and performing an eff ector function aimed to combat pathogens at the site infl ammation. At same time, NETs play important role in pathogenesis many autoimmune ammatory diseases as well malignancies. Rabbits one most commonly used species laboratory animals medical biological research. A large number models cardiovascular, immune other human systems have been developed rabbits. However, there is no information scientifi c literature about ability rabbit neutrophils undergo NETosis response well-known pharmacological stimuli. The purpose present work was study vitro system Soviet chinchilla form mimetic diacylglycerol phorbol 12-myristate 13-acetate (PMA) calcium ionophore A23187. To isolate neutrophils, one-step density gradient centrifugation on Ficoll-Hypaque method modifi cations used. Oxidative burst assessed luminol-amplifi ed chemiluminescence method, NET formation immunofl uorescence analysis. shows for fi rst time that do not PMA, but A23187, a low level oxidative A23187 chemoattractant N-formyl-methionylleucyl-phenylalanine.
Язык: Английский
Процитировано
0
Vestnik Moskovskogo universiteta Seria 16 Biologia, Год журнала: 2024, Номер 79(№1, 2024), С. 66 - 71
Опубликована: Июнь 3, 2024
Neutrophil extracellular traps (NETs) are decondensed nuclear chromatin, decorated with bactericidal proteins of various cell organelles and performing an eff ector function aimed to combat pathogens at the site infl ammation. At same time, NETs play important role in pathogenesis many autoimmune ammatory diseases as well malignancies. Rabbits one most commonly used species laboratory animals medical biological research. A large number models cardiovascular, immune other human systems have been developed rabbits. However, there is no information scientifi c literature about ability rabbit neutrophils undergo NETosis response well-known pharmacological stimuli. The purpose present work was study vitro system Soviet chinchilla form mimetic diacylglycerol phorbol 12-myristate 13-acetate (PMA) calcium ionophore A23187. To isolate neutrophils, one-step density gradient centrifugation on Ficoll-Hypaque method modifi cations used. Oxidative burst assessed luminol-amplifi ed chemiluminescence method, NET formation immunofl uorescence analysis. shows for fi rst time that do not PMA, but A23187, a low level oxidative A23187 chemoattractant N-formyl-methionylleucyl-phenylalanine.
Язык: Английский
Процитировано
0
Moscow University Biological Sciences Bulletin, Год журнала: 2024, Номер 79(1), С. 52 - 57
Опубликована: Март 1, 2024
Язык: Английский
Процитировано
0
Kazan medical journal, Год журнала: 2024, Номер 105(5), С. 701 - 708
Опубликована: Окт. 2, 2024
BACKGROUND: Ovarian cancer is the most aggressive gynecological tumor with high mortality. The ambivalent, both helper and suppressive effects of neutrophils on cells innate adaptive immunity determines their important immunoregulatory role in development malignant tumors. AIM: Evaluation effect serum interleukin-1β circulating CD16+ progression-free time advanced ovarian cancer. MATERIAL AND METHODS: In 42 primary patients serous adenocarcinoma (median age 54 years) ascites 15 benign tumors 60 admitted for examination treatment to Regional Clinical Oncology Dispensary before receiving neoadjuvant antitumor (2015–2020), level blood was determined using enzyme immunoassay, number by fluorescence microscopy monoclonal antibodies. Statistical processing performed Statistica 13 Jamovi 2.4.14 programs. Progression-free analysis Cox Kaplan–Meier regression methods. RESULTS: It found that lower than [48.0; 95% confidence interval (CI) 46.59–48.12 versus 50.0; CI 48.51–49.76; p=0.017]. At same time, higher [2.52 (95% 2.50–3.37) 1.26 0.97–1.55) (p=0.0001)]. multivariate (odds ratio 4.334; 1.83–10.23; p=0.001), neutrophil count 0.73; 0.62–0.86; p=0.0001) levels 1.90; 1.41–2.57; had prognostic significance assessing time. CONCLUSION: simultaneously affect
Язык: Английский
Процитировано
0