Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 19, 2025
The
underlying
cause
of
psoriasis,
a
chronic
inflammatory
skin
condition
driven
by
an
immune
response,
remains
topic
active
investigation
and
is
not
yet
fully
elucidated.
Recent
studies
have
revealed
that
ergothioneine,
small
molecule
sulfur-containing
histidine
derivative
can
be
ingested
from
the
daily
diet
accumulated
in
body,
exhibits
antioxidant
capacity
comparable
to
glutathione.
Nevertheless,
there
paucity
empirical
data
concerning
precise
impact
ergothioneine
context
anti-inflammatory
processes,
particularly
psoriasis.
In
light
aforementioned
considerations,
present
study
was
undertaken
with
objective
conducting
comprehensive
evaluation
potential
(EGT)
investigate
its
on
pathogenesis
efficacy
EGT
reducing
extent
dorsal
lesions
psoriasis
model
mice
confirmed
through
vivo
experimental
observation.
Furthermore,
inhibitory
effect
responses
at
cellular
level
investigated,
specifically
LPS-induced
mouse
macrophage
(RAW264.7)
human
keratinocyte-forming
cell
(HaCaT)
models.
results
demonstrated
introduction
different
concentrations
into
resulted
notable
effects,
as
evidenced
reduction
dose
dependent
decline
key
cytokines,
including
interleukin-1β
(IL-1β),
cyclooxygenase-2
(COX-2)
tumour
necrosis
factor-α
(TNF-α).
observed
reverse
LPS
induced
increase
ratio
M1
M2
macrophages.
also
markedly
suppress
phosphorylation
JAK/STAT3
NF-κB,
offering
novel
insight
mechanism
EGT.
conclusion,
findings
consistently
had
significant
ameliorative
imiquimod-induced
modulating
NF-κB/JAK-STAT3
signalling
pathway.
This
provides
strong
rationale
for
application
treatment.
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Март 20, 2025
Retinal
cell
apoptosis
is
the
primary
pathological
process
in
many
retinal
diseases,
including
retinitis
pigmentosa
and
age-related
macular
degeneration,
which
can
cause
severe
visual
impairment
blindness.
Lycium
barbarum
L.
,
a
traditional
Chinese
medicinal
botanical
drug,
has
long
history
extensive
application
ophthalmic
disease
prevention
treatment.
This
study
systematically
reviewed
key
active
metabolites
L.,
polysaccharides,
carotenoids,
flavonoids,
that
exert
protective
effects.
A
comprehensive
analysis
of
pharmacological
effects
underlying
molecular
mechanisms
its
treatment
apoptosis,
essential
aspects
such
as
antioxidant
activity,
anti-inflammatory
properties,
autophagy
regulation,
mitochondrial
function
preservation,
to
establish
solid
theoretical
basis
for
further
investigation
value
ophthalmology
provide
reference
future
research
directions.
Abstract
Background
Qingying
decoction
(QYD)
is
a
traditional
prescription
in
China
that
has
been
shown
to
be
effective
treating
psoriasis.
However,
its
mechanism
of
action
remains
elucidated.
Methods
The
active
ingredients
and
targets
QYD
were
obtained
from
TCMSP
database,
HERB
database
SwissTargetPrediction
respectively.
Differential
expression
gene
(DEGs)
analysis
weighted
co-expression
network
(WGCNA)
used
identify
key
genes
associated
with
Protein-protein
interaction
(PPI)
was
constructed
using
STRING
platform.
Gene
Ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
analyses
performed
the
DAVID
clusterProfiler
package
R
software.
Cytoscape
3.9.0
software
screen
components
hub
targets.
Molecular
docking
detect
binding
ability
between
An
vitro
model
psoriasis
established
by
stimulating
keratinocyte
HaCaT
mixture
five
pro-inflammatory
cytokines
(IL-17
A,
IL-22,
IL-1α,
oncostatin
M,
TNF-α)
(M5).
Cell
viability
cell
cycle
measured
counting
Kit
8
(CCK-8)
flow
cytometry,
Real-time
quantitative
polymerase
chain
reaction
(qRT-PCR)
mRNA
levels
genes,
high-proliferation
marker
keratin
6
(KRT6)
inflammatory
factors
IL-1β,
IL-6
TNF-α.
Protein
PI3K/AKT/FoxO
pathway
related
detected
Western
blot.
Results
A
total
139
screened
this
study,
1033
targets,
59
which
overlapped
psoriasis-related
genes.
Quercetin,
luteolin,
kaempferol,
beta-sitosterol
methylophiopogonanone
considered
treatment
CDC25A,
TOP2A,
NEK2
CCNA2
identified
could
probably
regulate
cycle,
T
receptor
signaling
metabolic
treat
had
good
affinity
target
proteins.
significantly
attenuated
M5-induced
hyperproliferation
progression
cells.
M5
stimulation
upregulates
NEK2,
CCNA2,
TNF-α,
while
reversed
effect.
In
addition,
inhibited
phosphorylation
PI3K
AKT
M5-stimulated
cells
upregulated
p-FOXO1
protein
level.
Conclusion
can
inhibit
excessive
proliferation
response
keratinocytes
regulating
pathway,
suggesting
may
an
attractive
for
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 15, 2025
Intricate
interactions
between
immune
cells
and
cytokines
define
psoriasis,
a
chronic
inflammatory
skin
condition
that
is
immunological-mediated.
Cytokines,
including
interleukins
(ILs),
interferons
(IFNs),
tumor
necrosis
factors
(TNFs),
chemokines,
transforming
growth
factor-β
(TGF-β),
are
essential
for
controlling
cellular
activity
immunological
responses,
maintaining
homeostasis
contributing
to
the
pathogenesis
of
psoriasis.
These
molecules
modulate
microenvironment
by
either
promoting
or
suppressing
inflammation,
which
significantly
impacts
therapeutic
outcomes.
Recent
research
indicates
treatment
strategies
targeting
chemokines
have
significant
potential,
offering
new
approaches
regulating
system,
inhibiting
progression
reducing
adverse
effects
traditional
therapies.
This
review
consolidates
current
knowledge
on
cytokine
chemokine
signaling
pathways
in
psoriasis
examines
their
significance
treatment.
Specific
attention
given
like
IL-17,
IL-23,
TNF-α,
underscoring
necessity
innovative
therapies
these
address
processes.
emphasizes
principal
part
-pathological
process
explores
challenges
opportunities
they
present
intervention.
Furthermore,
we
examine
recent
advancements
targeted
therapies,
with
particular
focus
monoclonal
antibodies,
ongoing
clinical
trials.
