Melatonin Modulates ZAP70 and CD40 Transcripts via Histone Modifications in Canine Ileum Epithelial Cells DOI Creative Commons
Jian Hong,

Saber Y. Adam,

Shiqi Wang

и другие.

Veterinary Sciences, Год журнала: 2025, Номер 12(2), С. 87 - 87

Опубликована: Янв. 23, 2025

Melatonin (MLT), produced by the pineal gland and other tissues, is known for its anti-inflammatory effects, particularly in regulating inflammatory markers cytokines intestinal cells. Our study aimed to investigate how MLT influences expression of genes through histone modification canine ileum epithelial cells (cIECs). In our experiment, cIECs were cultured divided into a control group (CON) an MLT-treatment group. did not significantly affect cell growth or death compared CON. However, treatment led upregulation CD40, ZAP70, IL7R downregulation LCK, RPL37, TNFRSF13B, CD4, CD40LG, BLNK, CIITA at mRNA level. Moreover, altered NF-kappa B signaling pathway upregulating genes, such as TICAM1, VCAMI, GADD45B, IRAK1, TRADD, RELA, RIPK1, RELB, downregulating PRKCB, LY96, ILIB, TNFRSF11A. Using ChIP-qPCR, we discovered that enhanced acetylation marks H3K9ac, H3K18ac, H3K27ac, methylation H3K4me1 H3K4me3 ZAP70 CD40 gene loci (p < 0.05). Additionally, enrichment RNA polymerase II phosphorylated Ser5 pol-II these was increased MLT-treated 0.05), indicating heightened transcriptional activity. conclusion, findings suggest mitigates inflammation modulating transcription modifications, offering potential therapeutic insights bowel diseases.

Язык: Английский

Melatonin Modulates ZAP70 and CD40 Transcripts via Histone Modifications in Canine Ileum Epithelial Cells DOI Creative Commons
Jian Hong,

Saber Y. Adam,

Shiqi Wang

и другие.

Veterinary Sciences, Год журнала: 2025, Номер 12(2), С. 87 - 87

Опубликована: Янв. 23, 2025

Melatonin (MLT), produced by the pineal gland and other tissues, is known for its anti-inflammatory effects, particularly in regulating inflammatory markers cytokines intestinal cells. Our study aimed to investigate how MLT influences expression of genes through histone modification canine ileum epithelial cells (cIECs). In our experiment, cIECs were cultured divided into a control group (CON) an MLT-treatment group. did not significantly affect cell growth or death compared CON. However, treatment led upregulation CD40, ZAP70, IL7R downregulation LCK, RPL37, TNFRSF13B, CD4, CD40LG, BLNK, CIITA at mRNA level. Moreover, altered NF-kappa B signaling pathway upregulating genes, such as TICAM1, VCAMI, GADD45B, IRAK1, TRADD, RELA, RIPK1, RELB, downregulating PRKCB, LY96, ILIB, TNFRSF11A. Using ChIP-qPCR, we discovered that enhanced acetylation marks H3K9ac, H3K18ac, H3K27ac, methylation H3K4me1 H3K4me3 ZAP70 CD40 gene loci (p < 0.05). Additionally, enrichment RNA polymerase II phosphorylated Ser5 pol-II these was increased MLT-treated 0.05), indicating heightened transcriptional activity. conclusion, findings suggest mitigates inflammation modulating transcription modifications, offering potential therapeutic insights bowel diseases.

Язык: Английский

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