Association of SIGLEC9 Expression with Cytokine Expression, Tumor Grading, KRAS, NRAS, BRAF, PIK3CA, AKT Gene Mutations, and MSI Status in Colorectal Cancer DOI Creative Commons
Błażej Ochman,

Anna Kot,

Sylwia Mielcarska

и другие.

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(12), С. 13617 - 13646

Опубликована: Ноя. 29, 2024

SIGLEC9 (sialic acid-binding Ig-like lectin 9) is a molecule thought to have significant influence on the immune properties of colorectal cancer (CRC) tumor microenvironment (TME). In our study, we assessed expression protein in CRC tissue and surgical margin tissue. Using RT-PCR, analyzed mutations KRAS, NRAS, BRAF, PIK3CA, AKT genes. We observed significantly elevated compared control group. No differences were depending genes or microsatellite instability (MSI) status. However, found higher high-grade tumors low-grade was associated with multiple cytokines, chemokines, growth factors TME. These associations suggest potential as that plays crucial role shaping TME, well its therapeutic relevance, particularly group tumors.

Язык: Английский

Tumor-Associated Macrophages as Major Immunosuppressive Cells in the Tumor Microenvironment DOI Open Access
Anghesom Ghebremedhin, Dipti Athavale,

Yanting Zhang

и другие.

Cancers, Год журнала: 2024, Номер 16(19), С. 3410 - 3410

Опубликована: Окт. 8, 2024

Within the tumor microenvironment, myeloid cells constitute a dynamic immune population characterized by heterogeneous phenotype and diverse functional activities. In this review, we consider recent literature shedding light on increasingly complex biology of M2-like immunosuppressive tumor-associated macrophages (TAMs), including their contribution to cell invasion metastasis, stromal remodeling (fibrosis matrix degradation), suppressive functions, in microenvironment (TME). This review also delves into intricate signaling mechanisms underlying polarization macrophage phenotypes, plasticity. We development promising therapeutic approaches target these populations cancers. The expanding knowledge distinct subsets TAMs, contributions tumorigenesis has sparked significant interest among researchers regarding potential TAM depletion or phenotypic modulation.

Язык: Английский

Процитировано

7

Targeting tumor-associated macrophages in colon cancer: mechanisms and therapeutic strategies DOI Creative Commons

Jianqin Xiang,

Jian Wang, Huihui Xiao

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Март 21, 2025

Colon cancer (CC) remains a primary contributor to cancer-related fatalities worldwide, driven by difficulties in early diagnosis and constrained therapeutic options. Recent studies underscore the importance of tumor microenvironment (TME), notably tumor-associated macrophages (TAMs), fostering malignancy progression therapy resistance. Through their inherent plasticity, TAMs facilitate immunosuppression, angiogenic processes, metastatic spread, drug tolerance. In contrast M1 macrophages, which promote inflammatory tumoricidal responses, M2 support expansion dissemination exerting immunosuppressive pro-angiogenic influences. Consequently, manipulating has emerged as potential avenue enhance treatment effectiveness. This review outlines origins, polarization states, functions CC, highlights role driving advancement, surveys ongoing efforts target these cells for better patient outcomes. Emerging strategies aimed at modulating TAM - including depletion strategies, reprogramming approaches that shift M2-polarized toward an phenotype, inhibition key signaling pathways sustaining TAM-mediated immunosuppression-are currently under active investigation. These hold promise overcoming induced resistance improving immunotherapeutic efficacy CC.

Язык: Английский

Процитировано

0

Research progress of tumor-associated macrophages in immune checkpoint inhibitor tolerance in colorectal cancer DOI
Fan Qi, Zhengwei Fu, Ming Xu

и другие.

World Journal of Gastrointestinal Oncology, Год журнала: 2024, Номер 16(10), С. 4064 - 4079

Опубликована: Сен. 25, 2024

The relevant mechanism of tumor-associated macrophages (TAMs) in the treatment colorectal cancer patients with immune checkpoint inhibitors (ICIs) is discussed, and application prospects TAMs reversing tolerance ICIs are discussed to provide a reference for related studies. As class drugs widely used clinical tumor immunotherapy, can act on regulatory molecules cells that play an inhibitory role-immune checkpoints-and kill tumors form response by activating variety system. sensitivity different types ICI varies greatly. phenotype function microenvironment closely efficacy ICIs. regulate phenotypic TAMs, also affect therapy. important role resistance, making full use this target as therapeutic strategy expected improve immunotherapy prognosis cancer.

Язык: Английский

Процитировано

1

Multi-omic profiling of breast tumor microenvironment uncovers a role of mitochondrial calcium gatekeepers DOI Creative Commons
Yen‐Dun Tony Tzeng, Pei‐Yi Chu, Su‐Boon Yong

и другие.

Journal of Cancer, Год журнала: 2024, Номер 15(12), С. 3663 - 3674

Опубликована: Янв. 1, 2024

In this study, we aimed to elucidate the role of mitochondrial calcium uptake 1/2 (MiCU1/2) in breast cancer (BRCA) by employing a comprehensive multi-omics approach.Unlike previous research, utilized novel web application tailored for whole tumor tissue, single-cell, and spatial transcriptomics analysis investigate association between MiCU1/2 immune microenvironment (TIME).Our gene set enrichment (GSEA) provided insights into primary biological effects MiCU1/2, while our CRISPR-based drug screening repository identified potential effective drugs.Our study revealed that high expression serves as an independent diagnostic biomarker, correlating with advanced clinical status indicating poorer recurrence-free survival (RFS) rates BRCA patients.Additionally, transcriptome highlighted heightened tumors its relevance surrounding cells.Furthermore, using CIBERSORT algorithm, discovered positive correlation levels macrophage infiltration, underscoring their impact on infiltration.We also patterns immune-related genes associated responses against various cell types, including CXCL, MIF, GDF, SPP1, IL16.Finally, pharmacogenomic small molecule drugs capable effectively targeting cells elevated expression.Overall, establishes promising biomarker diagnosis prognostic prediction, well therapeutic target, highlighting importance exploring these pathways advance patient care outcomes treatment.

Язык: Английский

Процитировано

0

Identification of potential drug targets for amyotrophic lateral sclerosis by Mendelian randomization analysis based on brain and plasma proteomics DOI Creative Commons

Ni Yang,

Liangyuan Shi,

Pengfei Xu

и другие.

Experimental Gerontology, Год журнала: 2024, Номер 195, С. 112538 - 112538

Опубликована: Авг. 9, 2024

Amyotrophic lateral sclerosis as a fatal neurodegenerative disease currently lacks effective therapeutic agents. Thus, finding new targets to drive treatment is necessary. In this study, we utilized brain and plasma proteins genetic instruments obtained from genome-wide association studies conduct Mendelian randomization analysis identify potential drug for amyotrophic sclerosis. Additionally, validated our results externally using other datasets. We also used Bayesian co-localization phenotype scanning. Furthermore, constructed protein-protein interaction network elucidate correlations between the identified existing targets. indicated that elevated levels of ANO5 (OR = 1.30; 95 % CI, 1.14–1.49; P 1.52E-04), SCFD1 3.82; 2.39–6.10; 2.19E-08), SIGLEC9 1.05; 95% 1.03–1.07; 4.71E-05) are associated with an increased risk sclerosis, external validation supporting these findings. Co-localization confirmed ANO5, SCFD1, (coloc.abf-PPH4 0.848, 0.984, 0.945, respectively) shared same variant further substantiating role target. There interactive relationships Our findings suggested connected might be promising However, exploration necessary fully understand underlying mechanisms involved.

Язык: Английский

Процитировано

0

Siglecs as modulators of macrophage phenotype and function DOI Creative Commons
E Kukán,

Gabrielle L Fabiano,

Brian A. Cobb

и другие.

Seminars in Immunology, Год журнала: 2024, Номер 73, С. 101887 - 101887

Опубликована: Май 1, 2024

Язык: Английский

Процитировано

0

ST6GAL1-mediated sialyl linkage switching in tumor-associated macrophages drives cancer-promoting nanotubes carrying α2,6-sialylation in anti-inflammatory cells DOI Open Access
Priya Dipta,

Naaz Bansal,

Zeynep Sumer‐Bayraktar

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 3, 2024

Abstract Tumor-associated macrophages (TAMs) form functionally diverse populations of innate immune cells in the tumor microenvironment (TME). Pro- and anti-inflammatory TAMs are central to cancer progression by shaping inflammation (im)balance, but it remains unknown if polarization-induced remodeling TAM glycocalyx critical for cellular communication occurs within TME. Taking a systems glycobiology approach, we here firstly used cell surface-focused glycomics lectin flow cytometry ex vivo polarized monocyte-derived demonstrate profound sialyl linkage switching surface N -glycome pro-inflammatory (α2,3-sialo-favored) (α2,6-sialo-dominant) macrophages. In contrast, no alterations sialylation were observed O -glycome. ST6GAL1 that modifies -glycans with α2,6-sialylation was elevated compared levels providing mechanistic basis switching, which supported silencing. Interestingly, SNA-focused cytochemistry revealed dense networks dynamic α2,6-sialylated protein-based nanotubules forming inter-connecting structures absent Temporal silencing caused nanotubule disintegration as evidenced SNA biotin fluorescence microscopy. Moreover, live recordings cultured without colorectal (CRC) showed reduced macrophage motility, attenuated inter-macrophage macrophage-CRC interactions diminished CRC proliferation upon disruption indicating functional roles nanotubules. Finally, recapitulated pro- from tissues patients advanced CRC. We report on consequences accompanying polarization.

Язык: Английский

Процитировано

0

Association of SIGLEC9 Expression with Cytokine Expression, Tumor Grading, KRAS, NRAS, BRAF, PIK3CA, AKT Gene Mutations, and MSI Status in Colorectal Cancer DOI Creative Commons
Błażej Ochman,

Anna Kot,

Sylwia Mielcarska

и другие.

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(12), С. 13617 - 13646

Опубликована: Ноя. 29, 2024

SIGLEC9 (sialic acid-binding Ig-like lectin 9) is a molecule thought to have significant influence on the immune properties of colorectal cancer (CRC) tumor microenvironment (TME). In our study, we assessed expression protein in CRC tissue and surgical margin tissue. Using RT-PCR, analyzed mutations KRAS, NRAS, BRAF, PIK3CA, AKT genes. We observed significantly elevated compared control group. No differences were depending genes or microsatellite instability (MSI) status. However, found higher high-grade tumors low-grade was associated with multiple cytokines, chemokines, growth factors TME. These associations suggest potential as that plays crucial role shaping TME, well its therapeutic relevance, particularly group tumors.

Язык: Английский

Процитировано

0