Targeting Pyroptosis: A Novel Strategy of Ginseng for the Treatment of Diabetes and Its Chronic Complications

Phytomedicine, Год журнала: 2025, Номер unknown, С. 156430 - 156430

Опубликована: Янв. 1, 2025

Язык: Английский

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Effective protective mechanisms of HO-1 in diabetic complications: a narrative review

Cell Death Discovery, Год журнала: 2024, Номер 10(1)

Опубликована: Окт. 10, 2024

Diabetes mellitus is a metabolic disorder with persistent hyperglycemia caused by variety of underlying factors. Chronic can lead to diverse serious consequences and diversified complications, which pose threat patients. Among the major complications are cardiovascular disease, kidney diabetic foot ulcers, retinopathy, neurological disorders. Heme oxygenase 1 (HO-1) protective enzyme antioxidant, anti-inflammatory anti-apoptotic effects, has been intensively studied plays an important role in complications. By inducing expression activity HO-1, it enhance anti-inflammatory, capacity tissues, thus reduce degree damage The present study aims review relationship between HO-1 pathogenesis diabetes its involved regulation macrophage polarization promotes M1 state (pro-inflammatory) towards M2 (anti-inflammatory). Induction dendritic cells inhibits them maturation secretion pro-inflammatory cytokines regulatory T cell (T

Язык: Английский

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Schisandrin A Attenuates Diabetic Nephropathy via EGFR/AKT/GSK3β Signaling Pathway Based on Network Pharmacology and Experimental Validation

Biology, Год журнала: 2024, Номер 13(8), С. 597 - 597

Опубликована: Авг. 8, 2024

Diabetic nephropathy (DN) is one of the common complications diabetes and main cause end-stage renal disease (ESRD) in clinical practice. Schisandrin A (Sch A) has multiple pharmacological activities, including inhibiting fibrosis, reducing apoptosis oxidative stress, regulating immunity, but its mechanism for treatment DN still unclear. In vivo, streptozotocin (STZ) a high-fat diet were used to induce type 2 diabetic rats, Sch was administered 4 weeks. At same time, protein–protein interaction (PPI) networks established analyze overlapping genes A. Subsequently, Kyoto Encyclopedia Genes Genomes (KEGG) Gene Ontology (GO) analyses performed determine hub pathway. addition, molecular docking preliminarily verify affinity proteins Further, H&E staining, Sirius red immunohistochemistry, immunofluorescence, western blot analysis detect location expression related DN. This study revealed multi-target multi-pathway characteristics First, could effectively improve glucose tolerance, reduce urine microprotein creatinine levels, alleviate pathological damage rats. Second, EGFR gene screened (43) (100) (2524). Finally, it that inhibit protein levels PTRF reduced apoptosis-related proteins, this effect modulation AKT/GSK-3β signaling summary, protective may be potential therapeutic target, throughout modulating

Язык: Английский

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Podocyte Death in Diabetic Kidney Disease: Potential Molecular Mechanisms and Therapeutic Targets

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(16), С. 9035 - 9035

Опубликована: Авг. 20, 2024

Cell deaths maintain the normal function of tissues and organs. In pathological conditions, abnormal activation or disruption cell death often leads to pathophysiological effects. Diabetic kidney disease (DKD), a significant microvascular complication diabetes, is linked high mortality morbidity rates, imposing substantial burden on global healthcare systems economies. Loss detachment podocytes are key changes in progression DKD. This review explores potential mechanisms apoptosis, necrosis, autophagy, pyroptosis, ferroptosis, cuproptosis, podoptosis podocytes, focusing how different modes contribute It recognizes limitations current research presents latest basic clinical studies targeting podocyte pathways Lastly, it focuses future treat DKD, with intention inspiring further development therapeutic strategies.

Язык: Английский

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Circulating Levels of miR-155 and CTBP1-AS2 as a promising biomarker for early detection of diabetic nephropathy

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Background Diabetic nephropathy (DN) is one of the most frequent complications diabetes mellitus. Since early diagnosis DN crucial to prevent progression disease towards renal failure, many efforts have been made in recent years introduce new diagnostic biomarkers. Recent studies suggest that non-coding RNAs could act as a novel biomarker for detection and prediction progress. Accordingly, current study we investigated expression levels miR-155 CTBP1-AS2 type 2 (T2D), patients control subjects evaluated their potential DN. Method A total 189 age sex-matched including 65 T2D with normo-albuminuria, 61 who had history albuminuria, 63 were included this case-control study. The determined using QRT-PCR. Results results revealed level was significantly reduced patients. In addition, higher macroalbuminuria compared microalbuminuria normo-albuminuria. without proteinuria than macroalbuminuria. also showed there significant positive correlation between DBP, TG, TC, SCr and, BUN negative HDL-C eGFR values. Conclusion Deregulation may represent useful biomarkers

Язык: Английский

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Dapagliflozin attenuates skeletal muscle atrophy in diabetic nephropathy mice through suppressing Gasdermin D-mediated pyroptosis

International Immunopharmacology, Год журнала: 2025, Номер 148, С. 114088 - 114088

Опубликована: Янв. 20, 2025

Язык: Английский

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Dapagliflozin Modulates MicroRNA Expression in Type 2 Diabetes Patients with Diabetic Nephropathy: A Retrospective Study

European Journal of Therapeutics, Год журнала: 2025, Номер 31(1), С. 1 - 10

Опубликована: Фев. 28, 2025

Objective: Diabetic nephropathy (DN) is one of the most serious microvascular complications Diabetes Mellitus, prevalence and mortality which are increasing. Recently, microRNAs (miRNAs) used in pathogenesis diagnosis many diseases have been identified. In this study investigate relationship between albuminuria miRNA levels patients with type 2 diabetes mellitus (T2DM) treated dapagliflozin, to elucidate potential nephroprotective effects dapagliflozin through modulation. Methods: This retrospective included 47 T2DM (25 male, 22 female) diabetic dapagliflozin. Blood samples were collected at baseline (day 0) after approximately 60 days treatment. Levels miRNA-21, miRNA-141, miRNA-377 analyzed using real-time PCR. Clinical laboratory parameters, including glucose, HbA1c, urine albumin protein levels, also assessed. Statistical analysis Wilcoxon signed-rank test Spearman's rank correlation test. Results: Significant decreases observed urinary protein, treatment (p<0.01). decreased significantly following A positive was found day 0 serum glucose levels. negative miRNA-21 HbA1c No significant correlations or Conclusion: Dapagliflozin DN associated reductions alongside improvements glycemic control renal function markers. These findings suggest that may exert its partly modulation DN-associated miRNAs. be a therapeutic option treat an effective strategy prevent kidney damage. Further research warranted mechanisms underlying these explore miRNAs as biomarkers targets management.

Язык: Английский

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Study on the Role of Schisandrin B in Ameliorating Hepatic Ischemia-Reperfusion Injury by Modulating Hepatocyte Autophagy

Deleted Journal, Год журнала: 2025, Номер 24(1)

Опубликована: Апрель 13, 2025

Background: Hepatic ischemia-reperfusion injury (HIRI) significantly affects the prognosis of liver surgery, such as hepatocellular carcinoma resection and transplantation. However, pathogenesis HIRI has not been fully elucidated, prevention treatment strategies remain challenging. Methods: A mouse model was established, schisandrin B (Sch B) used to intervene in HIRI. The effect Sch on assessed using hematoxylin eosin (HE) staining, quantitative polymerase chain reaction (qPCR), Western blot (WB), immunohistochemistry, enzyme-linked immunosorbent assay (ELISA). Results: expression alanine aminotransferase (ALT) aspartate (AST) serum increased, indicating successful construction model. In intervention group, ALT AST levels were decreased. Hematoxylin staining qPCR results demonstrated that could reduce mice. immunohistochemistry showed reduced mice by decreasing autophagy-related factors Beclin-1 LC3-II. Conclusions: Additionally, immunohistochemical indicated (Beclin-1 LC3-II) hepatocyte damage-related (caspase-3, caspase-9, Bax), thereby reducing electron microscopy, confirmed autophagy alleviate

Язык: Английский

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Puerarin raises exosomal miR- 342 - 3p by inhibiting lncRNA NEAT1 in umbilical cord mesenchymal stem cells to alleviate renal tubular epithelial cell pyroptosis in chronic renal failure

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 25, 2025

Язык: Английский

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LncRNA SNHG7/miR-181b-5p/TLR4 Activates Inflammation And Promotes Pyroptosis Through NF-κB Signaling in Diabetic Nephropathy

Inflammation, Год журнала: 2025, Номер unknown

Опубликована: Май 15, 2025

MiR-181b-5p plays a critical role in the pyroptosis and injury of kidney tubular cells diabetic disease (DKD). The long noncoding RNA (lncRNA) small nucleolar hostgene 7 (SNHG7) has been shown to bind inhibit function miR-181b-5p. However, precise SNHG7 DKD remains unclear. To address this, current study measured expression levels SNHG7, miR-181b-5p, Toll-like receptor 4 (TLR4) using RT-qPCR analysis renal biopsies from both normal individuals patients with DKD. An vitro model was subsequently established by exposing HK-2 high glucose (HG). In tissues HG-stimulated cells, TLR4 were significantly elevated, while miR-181b-5p markedly reduced. Knockdown resulted multiple beneficial effects: it effectively attenuated glucose-induced lactate dehydrogenase (LDH) leakage, restored cell viability, inhibited production inflammatory cytokines tumor necrosis factor alpha (TNF-α), interleukin 18 (IL-18), 1β (IL-1β), suppressed activation nucleotide-binding oligomerization domain (NOD)-like pyrin containing 3 (NLRP3)/acysteinyl aspartate-specific proteinase 1 (caspase-1)/gasdermin D (GSDMD) pathway. Mechanistically, functions as molecular sponge for directly targets TLR4, collectively regulating nuclear factor-kappaB (NF-κB) pathway activation. Moreover, inhibition or up-regulation reversed protective effects knockdown. Additionally, co-transfection over-expression vector mimic counteracted overexpression on LDH factors pyroptosis-related molecules. summary, acts promoting inflammation DKD, which turn regulates NF-κB signaling

Язык: Английский

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