N4-acetylcytidine and other RNA modifications in epitranscriptome: insight into DNA repair and cancer development
Epigenomics,
Год журнала:
2025,
Номер
unknown, С. 1 - 12
Опубликована: Март 5, 2025
N4-acetylcytidine
(ac4C)
is
a
post-transcriptional
RNA
modification
that
plays
crucial
role
in
the
epitranscriptome,
influencing
gene
expression
and
cellular
function.
This
occurs
at
cytosine
base,
where
an
acetyl
group
installed
to
nitrogen
4th
position
(N4).
co-transcription
affects
stability,
structure,
translation
efficiency.
Recent
studies
have
uncovered
potential
link
between
modifications
DNA
repair
mechanisms,
suggesting
ac4C-modified
or
methylated
RNAs
may
interact
with
factors
involved
pathways;
thus,
response
damage.
Dysregulation
of
modified
RNAs,
including
ac4C
RNA,
has
been
implicated
cancer
development,
aberrant
levels
these
contribute
oncogenic
transformation
by
altering
genome
stability
key
genes
regulating
cell
proliferation,
cycle
progression,
apoptosis.
Understanding
dynamics
offers
promising
insights
into
epitranscriptome
processes
treatment.
Язык: Английский
NAT10 mediates TLR2 to promote podocyte senescence in adriamycin-induced nephropathy
Cell Death and Disease,
Год журнала:
2025,
Номер
16(1)
Опубликована: Март 19, 2025
Abstract
N-acetyltransferase
10
(NAT10)
is
involved
in
regulating
senescence.
However,
its
role
glomerular
diseases
remains
unclear.
Therefore,
this
study
aims
to
investigate
the
mechanisms
by
which
NAT10
influences
senescence
and
damage
an
adriamycin
(ADR)-induced
nephropathy
model.
Senescence
(p16
p21)
DNA
markers
(γ-H2AX
(ser139))
were
assessed
ADR-induced
nephropathy.
function
was
demonstrated
using
Remodelin
or
small
interfering
RNA
(siRNA)
interventions.
Transcriptome
sequencing
conducted
identify
key
downstream
genes
pathways,
while
coimmunoprecipitation
performed
evaluate
relationship
between
toll-like
receptor
2
(TLR2)
expression.
TLR2
overexpression
knockdown
further
validated
regulatory
In
ADR-treated
mice,
expression
levels
of
P53,
P21,
P16,
γ-H2AX(S139)
proteins
elevated,
those
WT-1
nephrin
reduced.
This
effect
mitigated
siNAT10
administration.
identified
as
a
gene,
coimmunoprecipitation,
along
with
molecular
docking
models,
confirmed
interaction
NAT10.
plasmid
siRNA
employed
for
recovery
experiments.
Together,
findings
suggest
that
contributes
podocyte
injury
via
TLR2.
Further,
it
demonstrates
alleviates
interacting
TLR2,
potentially
through
P53-P21-dependent
mechanism.
Thus
could
serve
novel
therapeutic
target
treating
proteinuric
glomerulopathies.
Язык: Английский
Sitagliptin regulates the AMPK/NF-κB signaling pathway to alleviate lipopolysaccharide-induced inflammatory responses and promote osteogenic differentiation in rat bone marrow mesenchymal stem cells
Archives of Oral Biology,
Год журнала:
2025,
Номер
unknown, С. 106253 - 106253
Опубликована: Апрель 1, 2025
This
study
aimed
to
explore
the
impact
of
sitagliptin
on
inflammatory
response
and
osteogenic
differentiation
in
lipopolysaccharide
(LPS)-stimulated
rat
bone
marrow
mesenchymal
stem
cells
(BMSCs)
clarify
underlying
mechanisms
action.
In
vitro-cultured
BMSCs
were
identified,
treated
with
a
range
doses,
assessed
cell
counting
kit-8
assay
quantify
viability.
The
expression
proteins
genes
relevant
inflammation
osteogenesis
was
measured
using
enzyme-linked
immunosorbent
real-time
quantitative
polymerase
chain
reaction
techniques.
ability
analyzed
by
alkaline
phosphatase
staining,
activity
assay,
alizarin
red
s
staining.
Adenosine
monophosphate-activated
protein
kinase
(AMPK)/nuclear
factor-kappa
B
(NF-κB)
signaling
pathway
activation
detected
through
western
blotting.
High
but
not
low
concentrations
significantly
suppressed
cellular
Sitagliptin
dose-dependently
inhibited
LPS-induced
responses
while
facilitating
their
differentiation.
It
also
activated
AMPK
suppressing
NF-κB
activity.
inhibitor
treatment
partially
reversed
these
beneficial
effects
osteogenesis.
suppresses
promoting
modulation
AMPK/NF-κB
activity,
thereby
mitigating
functional
impairment
under
microenvironmental
conditions.
Язык: Английский
Metabolism-epigenetic interaction-based bone and dental regeneration: From impacts and mechanisms to treatment potential
Bone,
Год журнала:
2024,
Номер
unknown, С. 117382 - 117382
Опубликована: Дек. 1, 2024
Язык: Английский
LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts
Molecular Medicine Reports,
Год журнала:
2024,
Номер
30(6)
Опубликована: Окт. 15, 2024
Addressing
the
repair
and
regeneration
of
large
bone
defects
poses
significant
challenges
in
tissue
engineering.
Despite
abundant
evidence
demonstrating
positive
role
MSCs
osteogenesis,
their
limited
osteogenic
differentiation
ability
still
needs
to
be
improved.
The
present
study
used
lipopolysaccharide
(LPS)
enhance
properties
ecto‑mesenchymal
stem
cells
(EMSCs).
Human
nasal
respiratory
mucosa‑derived
EMSCs
were
cultured
on
plates
stimulated
with
LPS
for
5
days
prior
undergoing
differentiation.
findings
revealed
that
effectively
capacity
EMSCs,
as
evidenced
by
heightened
alkaline
phosphatase
activity,
elevated
expression
levels
osteogenic‑related
proteins
enhanced
mineralization
EMSCs.
also
demonstrated
augmentation
occurred
due
increased
IL‑10
levels,
although
it
was
not
solely
attributable
this
factor.
Together,
illustrated
LPS‑mediated
adaptation
is
an
active
process
driving
could
a
novel
strategy
regeneration.
Язык: Английский