LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts DOI Creative Commons

Demin Lv,

Bingxia Li, Zhen Liu

и другие.

Molecular Medicine Reports, Год журнала: 2024, Номер 30(6)

Опубликована: Окт. 15, 2024

Addressing the repair and regeneration of large bone defects poses significant challenges in tissue engineering. Despite abundant evidence demonstrating positive role MSCs osteogenesis, their limited osteogenic differentiation ability still needs to be improved. The present study used lipopolysaccharide (LPS) enhance properties ecto‑mesenchymal stem cells (EMSCs). Human nasal respiratory mucosa‑derived EMSCs were cultured on plates stimulated with LPS for 5 days prior undergoing differentiation. findings revealed that effectively capacity EMSCs, as evidenced by heightened alkaline phosphatase activity, elevated expression levels osteogenic‑related proteins enhanced mineralization EMSCs. also demonstrated augmentation occurred due increased IL‑10 levels, although it was not solely attributable this factor. Together, illustrated LPS‑mediated adaptation is an active process driving could a novel strategy regeneration.

Язык: Английский

N4-acetylcytidine and other RNA modifications in epitranscriptome: insight into DNA repair and cancer development DOI Creative Commons
Eva Bártová, Lenka Stixová,

Alena Svobodová Kovaříková

и другие.

Epigenomics, Год журнала: 2025, Номер unknown, С. 1 - 12

Опубликована: Март 5, 2025

N4-acetylcytidine (ac4C) is a post-transcriptional RNA modification that plays crucial role in the epitranscriptome, influencing gene expression and cellular function. This occurs at cytosine base, where an acetyl group installed to nitrogen 4th position (N4). co-transcription affects stability, structure, translation efficiency. Recent studies have uncovered potential link between modifications DNA repair mechanisms, suggesting ac4C-modified or methylated RNAs may interact with factors involved pathways; thus, response damage. Dysregulation of modified RNAs, including ac4C RNA, has been implicated cancer development, aberrant levels these contribute oncogenic transformation by altering genome stability key genes regulating cell proliferation, cycle progression, apoptosis. Understanding dynamics offers promising insights into epitranscriptome processes treatment.

Язык: Английский

Процитировано

0

NAT10 mediates TLR2 to promote podocyte senescence in adriamycin-induced nephropathy DOI Creative Commons
Mingyang Hu, Linxiao Lv, Yifeng Lei

и другие.

Cell Death and Disease, Год журнала: 2025, Номер 16(1)

Опубликована: Март 19, 2025

Abstract N-acetyltransferase 10 (NAT10) is involved in regulating senescence. However, its role glomerular diseases remains unclear. Therefore, this study aims to investigate the mechanisms by which NAT10 influences senescence and damage an adriamycin (ADR)-induced nephropathy model. Senescence (p16 p21) DNA markers (γ-H2AX (ser139)) were assessed ADR-induced nephropathy. function was demonstrated using Remodelin or small interfering RNA (siRNA) interventions. Transcriptome sequencing conducted identify key downstream genes pathways, while coimmunoprecipitation performed evaluate relationship between toll-like receptor 2 (TLR2) expression. TLR2 overexpression knockdown further validated regulatory In ADR-treated mice, expression levels of P53, P21, P16, γ-H2AX(S139) proteins elevated, those WT-1 nephrin reduced. This effect mitigated siNAT10 administration. identified as a gene, coimmunoprecipitation, along with molecular docking models, confirmed interaction NAT10. plasmid siRNA employed for recovery experiments. Together, findings suggest that contributes podocyte injury via TLR2. Further, it demonstrates alleviates interacting TLR2, potentially through P53-P21-dependent mechanism. Thus could serve novel therapeutic target treating proteinuric glomerulopathies.

Язык: Английский

Процитировано

0

Sitagliptin regulates the AMPK/NF-κB signaling pathway to alleviate lipopolysaccharide-induced inflammatory responses and promote osteogenic differentiation in rat bone marrow mesenchymal stem cells DOI Creative Commons
Xi Yao, Min Liu, Pei Wang

и другие.

Archives of Oral Biology, Год журнала: 2025, Номер unknown, С. 106253 - 106253

Опубликована: Апрель 1, 2025

This study aimed to explore the impact of sitagliptin on inflammatory response and osteogenic differentiation in lipopolysaccharide (LPS)-stimulated rat bone marrow mesenchymal stem cells (BMSCs) clarify underlying mechanisms action. In vitro-cultured BMSCs were identified, treated with a range doses, assessed cell counting kit-8 assay quantify viability. The expression proteins genes relevant inflammation osteogenesis was measured using enzyme-linked immunosorbent real-time quantitative polymerase chain reaction techniques. ability analyzed by alkaline phosphatase staining, activity assay, alizarin red s staining. Adenosine monophosphate-activated protein kinase (AMPK)/nuclear factor-kappa B (NF-κB) signaling pathway activation detected through western blotting. High but not low concentrations significantly suppressed cellular Sitagliptin dose-dependently inhibited LPS-induced responses while facilitating their differentiation. It also activated AMPK suppressing NF-κB activity. inhibitor treatment partially reversed these beneficial effects osteogenesis. suppresses promoting modulation AMPK/NF-κB activity, thereby mitigating functional impairment under microenvironmental conditions.

Язык: Английский

Процитировано

0

Metabolism-epigenetic interaction-based bone and dental regeneration: From impacts and mechanisms to treatment potential DOI
Xinyi Chen,

Xiaoyuan Huang,

Xiatong Zhang

и другие.

Bone, Год журнала: 2024, Номер unknown, С. 117382 - 117382

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

2

LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts DOI Creative Commons

Demin Lv,

Bingxia Li, Zhen Liu

и другие.

Molecular Medicine Reports, Год журнала: 2024, Номер 30(6)

Опубликована: Окт. 15, 2024

Addressing the repair and regeneration of large bone defects poses significant challenges in tissue engineering. Despite abundant evidence demonstrating positive role MSCs osteogenesis, their limited osteogenic differentiation ability still needs to be improved. The present study used lipopolysaccharide (LPS) enhance properties ecto‑mesenchymal stem cells (EMSCs). Human nasal respiratory mucosa‑derived EMSCs were cultured on plates stimulated with LPS for 5 days prior undergoing differentiation. findings revealed that effectively capacity EMSCs, as evidenced by heightened alkaline phosphatase activity, elevated expression levels osteogenic‑related proteins enhanced mineralization EMSCs. also demonstrated augmentation occurred due increased IL‑10 levels, although it was not solely attributable this factor. Together, illustrated LPS‑mediated adaptation is an active process driving could a novel strategy regeneration.

Язык: Английский

Процитировано

1