Blood transcriptomics identifies FEZ1 as a novel non-invasive diagnostic biomarker for inflammatory bowel disease DOI
Arman Mokaram Doust Delkhah

Опубликована: Окт. 28, 2024

Abstract In comparison with conventional diagnostic methods, blood biomarkers are an easily accessible source for diagnosing inflammatory bowel disease (IBD). To identify such a biomarker, integrated transcriptomics approach was employed to detect RNAs exhibiting efficacy. For this purpose, four gene expression datasets obtained from specimens were (GSE119600, GSE94648, GSE86434, and GSE71730). After determining differentially expressed genes in Crohn’s ulcerative colitis, DEGs IBD defined as consistent direction of alteration both disorders. Mapping the PPI network these revealed TNF central hub gene. Subsequently, weighted gene-expression analysis (WGCNA) carried out determine IBD-specific modules. Considering degree metrics, module membership, significance, PRF1 only discerned that prominently enriched IBD. Genes converging results differential WGCNA subjected random forest decision tree-based LASSO regression methods. Following identification FEZ1 NLRC5 highlighted by analyses, ROC applied assess their potential. Although demonstrated acceptable efficacy data, considered potential biomarker based on replication validation While autophagy is currently most convincing explanation involvement IBD, further investigations required elucidate its immunological role.

Язык: Английский

Skin Metabolic Signatures of Psoriasis and Psoriasis Concurrent with Metabolic Syndrome DOI Creative Commons
Liang Yan, W Wang,

Mei-Han Dong

и другие.

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 505 - 517

Опубликована: Янв. 1, 2025

Purpose: Psoriasis is a complex inflammatory skin disorder that closely associated with metabolic syndrome (MetS). Limited information available on changes in psoriasis; the effect of concurrent MetS psoriatic metabolite levels unknown. We aimed to expand this through metabolomic analysis. Patients and Methods: Untargeted metabolomics was conducted using samples from 38 patients psoriasis vulgaris (PVMS), 23 without (PVNMS), 10 healthy controls (HC). Data analyses, including multivariate statistical analysis, KEGG pathway enrichment correlation receiver operating characteristic curve were performed. Results: Significant discrepancies found between metabolites HC PVNMS groups, particularly those involved nucleotide glycerophospholipid metabolism. Fifteen these positively correlated severity. Furthermore, affect profiles psoriasis. There some consistent alterations both PVNMS/HC PVMS/PVNMS comparisons. Conclusion: This study may provide new insights into link metabolism inflammation mechanism underlying interaction MetS. Keywords: psoriasis, syndrome, metabolomics, metabolism,

Язык: Английский

Процитировано

0
Mammalian nucleophagy: process and function DOI
Fujian Ji, Enyong Dai, Rui Kang

и другие.

Autophagy, Год журнала: 2025, Номер unknown

Опубликована: Янв. 19, 2025

The nucleus is a highly specialized organelle that houses the cell's genetic material and regulates key cellular activities, including growth, metabolism, protein synthesis, cell division. Its structure function are tightly regulated by multiple mechanisms to ensure integrity genomic stability. Increasing evidence suggests nucleophagy, selective form of autophagy targets nuclear components, plays critical role in preserving clearing dysfunctional materials such as proteins (lamins, SIRT1, histones), DNA-protein crosslinks, micronuclei, chromatin fragments. Impaired nucleophagy has been implicated aging various pathological conditions, cancer, neurodegeneration, autoimmune disorders, neurological injury. In this review, we focus on mammalian cells, discussing its mechanisms, regulation, cargo selection, well evaluating therapeutic potential promoting human health mitigating disease.

Язык: Английский

Процитировано

0
Normal dermal mesenchymal stem cells improve the functions of psoriatic keratinocytes by inducing autophagy DOI

Nannan Liang,

Yue Cao, Junqin Li

и другие.

Acta Histochemica, Год журнала: 2025, Номер 127(1), С. 152229 - 152229

Опубликована: Янв. 25, 2025

Язык: Английский

Процитировано

0
Blood transcriptomics identifies FEZ1 as a potential biomarker for inflammatory bowel disease DOI
Arman Mokaram Doust Delkhah

Computers in Biology and Medicine, Год журнала: 2025, Номер 187, С. 109742 - 109742

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
How Cells Die in Psoriasis? DOI Open Access
Chung-Han Chen, Nan‐Lin Wu, Tsen‐Fang Tsai

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3747 - 3747

Опубликована: Апрель 16, 2025

Psoriasis, a chronic immune-mediated inflammatory skin disorder characterized by keratinocyte hyperproliferation and cell infiltration, involves multiple distinct programmed death pathways in its pathogenesis. Following the Nomenclature Committee on Cell Death recommendations, we analyzed current literature examining diverse modes of cellular psoriatic lesions, with particular focus patterns their molecular signatures. Analysis revealed several mechanisms: autophagy dysfunction through IL-17A pathways, decreased apoptotic activity lesional skin, medication targeting anoikis psoriasis, upregulated necroptosis mediated RIPK1/MLKL signaling, gasdermin-mediated pyroptosis enhanced IL-1β secretion, coordinated PANoptotic activation specialized complexes, PARP1-mediated parthanatos promoting cutaneous inflammation, iron-dependent ferroptosis correlating Th22/Th17 responses, copper-dependent cuproptosis elevated MTF1/ATP7B/SLC31A1 expression, NETosis amplifying immune responses interaction Th17 axis. The intricate interplay between these mechanisms has led to development targeted therapeutic strategies, including mTOR inhibitors for modulation, RIPK1 necroptosis, various approaches NETosis, providing new directions more effective psoriasis treatments.

Язык: Английский

Процитировано

0
Astragaloside IV suppresses the proliferation and inflammatory response of human epidermal keratinocytes and ameliorates imiquimod-induced psoriasis-like skin damage in mice DOI Open Access
Ting Liu, Lin Ai,

Aibo Jiang

и другие.

Allergologia et Immunopathologia, Год журнала: 2024, Номер 52(5), С. 44 - 50

Опубликована: Сен. 1, 2024

The primary pathological features of psoriasis include excessive epidermal keratinocytes and infiltration inflammatory cells, which are pivotal targets for therapy. Astragaloside IV (AS-IV), the principal active compound astragalus, exhibits anti-inflammatory, antioxidant, immune-modulatory properties. This study aims to investigate AS-IV's anti--psoriatic effects underlying mechanisms. Normal human (NHEKs) were stimulated with a combination TNF-α, IL-17A, IL-1α, IL-22, oncostatin M (M5) replicate psoriatic keratinocyte pathology

Язык: Английский

Процитировано

0
Blood transcriptomics identifies FEZ1 as a novel non-invasive diagnostic biomarker for inflammatory bowel disease DOI
Arman Mokaram Doust Delkhah

Опубликована: Окт. 28, 2024

Abstract In comparison with conventional diagnostic methods, blood biomarkers are an easily accessible source for diagnosing inflammatory bowel disease (IBD). To identify such a biomarker, integrated transcriptomics approach was employed to detect RNAs exhibiting efficacy. For this purpose, four gene expression datasets obtained from specimens were (GSE119600, GSE94648, GSE86434, and GSE71730). After determining differentially expressed genes in Crohn’s ulcerative colitis, DEGs IBD defined as consistent direction of alteration both disorders. Mapping the PPI network these revealed TNF central hub gene. Subsequently, weighted gene-expression analysis (WGCNA) carried out determine IBD-specific modules. Considering degree metrics, module membership, significance, PRF1 only discerned that prominently enriched IBD. Genes converging results differential WGCNA subjected random forest decision tree-based LASSO regression methods. Following identification FEZ1 NLRC5 highlighted by analyses, ROC applied assess their potential. Although demonstrated acceptable efficacy data, considered potential biomarker based on replication validation While autophagy is currently most convincing explanation involvement IBD, further investigations required elucidate its immunological role.

Язык: Английский

Процитировано

0