Promoting Radial Repair in Rabbits through BMP-9 Overexpression in Adipose-Derived Stem Cells Loaded with ICA/nHAC/PLA DOI Creative Commons

Fang-Tian Xu,

Lin Wang,

Shuang-Yi Li

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 12, 2024

Abstract Background Bone defects commonly cause delayed bone healing and local dysfunction. Both adipose-derived stem cells (ADSCs) icariin (ICA)-loaded nano-hydroxyapatite/collagen/polylactic acid (nHAC/PLA) have been reported to promote osteoblast metabolism accelerate fracture healing. Aim The aim of this study was explore the mechanism ADSCs combined with ICA-loaded nHAC/PLA in regulating defects. Methods effects ICA BMP-9 (Bone morphogenetic protein-9) overexpression on cell viability osteogenic differentiation were measured by qRT-PCR, western blotting alkaline phosphatase (ALP) assay. Alizarin red staining examined formation mineralized nodules ADSCs. Finally, radial defect rabbit model established, administrated nHAC/PLA. new detected X-ray, immunohistochemistry Hematoxylin eosin staining. Results significantly increased ALP activity nodule ADSCs, that amplified ICA. This combination also upregulated expression markers (BMP-9, OCN, OPN, COL-1, BSP, Runx-2) influenced key signaling pathways (p38 MAPK, JNK, ERK). In vivo, treatment group showed enhanced repair. Conclusions Our findings indicate BMP-9-overexpressed scaffolds is effective promoting repair, offering a potential therapeutic strategy for Core tip promoted proliferation ADSCs. BMP-9 osteogenesis ADSCs

Язык: Английский

Icariin modulates osteogenic and adipogenic differentiation in ADSCs via the Hippo-YAP/TAZ pathway: a novel therapeutic strategy for osteoporosis DOI Creative Commons

Shaozi Lin,

Zuyu Meng,

Mei Wang

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 15

Опубликована: Янв. 13, 2025

Adipose-derived stem cell (ADSC) transplantation presents a promising approach for osteoporosis (OP) treatment. However, the therapeutic efficacy of ADSCs is hindered by low post-transplantation survival rates and limited capacities adhesion, migration, differentiation. Icariin (ICA), primary active compound Epimedium, has been shown to promote proliferation induce osteogenic differentiation; however, its specific effects on ADSC osteogenesis mechanisms which ICA enhances treatment through remain inadequately understood. This study investigates different concentrations adipogenic differentiation rat ADSCs, aiming elucidate underlying mechanisms. were isolated from female SPF-grade SD rats, with surface markers identified flow cytometry. Osteogenic assessed using Alizarin Red Oil O staining, respectively. Third-generation divided into five groups: control, resveratrol (100 μmol/L), four groups (1, 10, 50, 100 μmol/L). Western blotting was performed analyze expression factors associated Hippo-YAP/TAZ signaling pathway marker PPARγ. Additionally, labeled lentiviruses carrying enhanced green fluorescent protein (EGFP) 5-bromo-2-deoxyuridine (BrdU) assess their in vivo distribution, survival, proliferation, post-ICA intervention. In vitro, significantly inhibited Hippo pathway, reducing YAP TAZ phosphorylation enhancing transcriptional activity, while simultaneously suppressing promoted adipogenesis ADSCs. vivo, ICA-treated demonstrated effective following subcutaneous injection allogeneic rats. Our demonstrates that inhibiting adipogenesis, providing novel insights strategies related conditions.

Язык: Английский

Процитировано

0
Icariin-Enhanced Osteoclast-Derived Exosomes Promote Repair of Infected Bone Defects by Regulating Osteoclast and Osteoblast Communication DOI Creative Commons
Yang Zhang, Minjie Zhang, Mengying Li

и другие.

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 12389 - 12407

Опубликована: Ноя. 1, 2024

Infected bone defects pose a challenging clinical issue due to an imbalance of osteoclasts (OC) and osteoblasts (OB). Exosomes are crucial for intercellular signaling OC OB in repair. Icariin, has been shown regulate the balance between OB. However, specific mechanisms by which icariin influences exosomes derived from osteoclasts, subsequently impacts osteoblast activity, remain unclear. This study aims investigate effects icariin-treated osteoclast-derived (ICA-OC-Exo) on function repair cases infected defects.

Язык: Английский

Процитировано

0
Promoting Radial Repair in Rabbits through BMP-9 Overexpression in Adipose-Derived Stem Cells Loaded with ICA/nHAC/PLA DOI Creative Commons

Fang-Tian Xu,

Lin Wang,

Shuang-Yi Li

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 12, 2024

Abstract Background Bone defects commonly cause delayed bone healing and local dysfunction. Both adipose-derived stem cells (ADSCs) icariin (ICA)-loaded nano-hydroxyapatite/collagen/polylactic acid (nHAC/PLA) have been reported to promote osteoblast metabolism accelerate fracture healing. Aim The aim of this study was explore the mechanism ADSCs combined with ICA-loaded nHAC/PLA in regulating defects. Methods effects ICA BMP-9 (Bone morphogenetic protein-9) overexpression on cell viability osteogenic differentiation were measured by qRT-PCR, western blotting alkaline phosphatase (ALP) assay. Alizarin red staining examined formation mineralized nodules ADSCs. Finally, radial defect rabbit model established, administrated nHAC/PLA. new detected X-ray, immunohistochemistry Hematoxylin eosin staining. Results significantly increased ALP activity nodule ADSCs, that amplified ICA. This combination also upregulated expression markers (BMP-9, OCN, OPN, COL-1, BSP, Runx-2) influenced key signaling pathways (p38 MAPK, JNK, ERK). In vivo, treatment group showed enhanced repair. Conclusions Our findings indicate BMP-9-overexpressed scaffolds is effective promoting repair, offering a potential therapeutic strategy for Core tip promoted proliferation ADSCs. BMP-9 osteogenesis ADSCs

Язык: Английский

Процитировано

0