Stigmasterol from Prunella vulgaris L. Alleviates LPS-Induced Mammary Gland Injury by Inhibiting Inflammation and Ferroptosis
Phytomedicine,
Год журнала:
2025,
Номер
137, С. 156362 - 156362
Опубликована: Янв. 5, 2025
Язык: Английский
Ferroptosis-Related Genes as Molecular Markers in Bovine Mammary Epithelial Cells Challenged with Staphylococcus aureus
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2506 - 2506
Опубликована: Март 11, 2025
Staphylococcus
aureus-induced
mastitis
is
a
significant
cause
of
economic
losses
in
the
dairy
industry,
yet
its
molecular
mechanisms
remain
poorly
defined.
Although
ferroptosis,
regulated
cell
death
process,
associated
with
inflammatory
diseases,
role
bovine
unknown.
In
this
study,
11
S.
aureus
strains
were
isolated
from
milk
samples
obtained
cows
clinical
or
subclinical
mastitis.
Transcriptome
analysis
Mac-T
cells
challenged
identified
differentially
expressed
genes
(DEGs).
Enrichment
revealed
associations
between
DEG
clusters
and
traits
related
to
KEGG
pathway
enrichment
Toll-like
receptor,
TNF
signaling
as
significantly
enriched
pathways.
Weighted
gene
co-expression
network
(WGCNA)
further
prioritized
ferroptosis-related
(HMOX1,
SLC11A2,
STEAP3,
SAT1,
VDAC2)
involved
iron
metabolism.
Notably,
expression
levels
HMOX1
SAT1
increased
aureus-challenged
cells,
upregulation
was
consistent
trends
observed
transcriptome
data
mother–daughter
pairs
caused
by
infection.
Furthermore,
Ferrostatin-1
treatment
reduced
confirming
involvement
ferroptosis
process.
This
study
reveals
that
plays
key
highlights
potential
target
for
breeding
strategies
aimed
at
improving
resistance.
Язык: Английский
Gardenoside attenuates Staphylococcus aureus -induced mastitis by inhibiting inflammation and ferroptosis through Nrf2/SLC7A11/GPX4 signaling pathway
Microbiology Spectrum,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 29, 2024
ABSTRACT
The
occurrence
of
mastitis
is
mainly
due
to
the
infection
mammary
tissue
by
pathogens,
which
causes
inflammation
tissue.
Gardenoside
(GAD),
an
iridoid
active
ingredient
extracted
from
Gardenia
Jasminoides
Ellis
,
has
been
revealed
exhibit
anti-inflammatory
and
anti-oxidative
roles.
However,
therapeutic
effect
GAD
on
remains
unclear.
Our
aim
was
identify
Staphylococcus
aureus
(S.
)-induced
clarify
its
mechanism.
To
carry
out
this
work,
S.
-induced
mice
model
established.
Enzyme-Linked
Immunosorbent
Assay
(ELISA)
conducted
detect
production
Interleukin-1β
(IL-1β)
tumor
necrosis
factor-α
(TNF-α).
biochemical
method
used
antioxidant
factors
glutathione
(GSH),
malondialdehyde
(MDA),
iron
content.
Western
blot
expression
Nrf2/SLC7A11/GPX4
signal-related
proteins.
results
demonstrated
that
alleviated
Myeloperoxidase
(MPO)
activity,
IL-1β
TNF-α
production,
NF-κB
activation.
MDA
contents
were
also
inhibited
GAD.
Meanwhile,
GSH
level
GPX4,
SLC7A11,
ferritin
expressions
increased
treatment.
In
addition,
Nrf2
HO-1
upregulated
conclusion,
GAP
may
inhibit
in
triggering
signaling
pathway
alleviating
ferroptosis.
IMPORTANCE
Mastitis,
as
important
disease
endangers
development
dairy
industry,
huge
economic
losses
breeding
industry.
one
pathogenic
bacteria
cause
mastitis.
Antibiotics
are
considered
be
first
choice
treatment
overuse
antibiotics
leads
bacterial
resistance
antibiotic
residues.
Therefore,
study
explored
whether
effective
extracts
traditional
herbs
could
alternatives
antibiotics.
Язык: Английский
Modulatory Effects of Regulated Cell Death: An Innovative Preventive Approach for the Control of Mastitis
Cells,
Год журнала:
2024,
Номер
13(20), С. 1699 - 1699
Опубликована: Окт. 14, 2024
Mastitis
is
a
common
disease
worldwide
that
affects
the
development
of
dairy
industry
due
to
its
high
incidence
and
complex
etiology.
Precise
regulation
cell
death
survival
plays
critical
role
in
maintaining
internal
homeostasis,
organ
development,
immune
function
organisms,
regulatory
abnormalities
are
mechanism
various
pathological
changes.
Recent
research
has
shown
regulated
(RCD)
crucial
mastitis.
The
drugs
treat
can
be
widely
used
mastitis
treatment
important
clinical
significance.
This
paper
will
review
molecular
mechanisms
apoptosis,
autophagy,
pyroptosis,
ferroptosis,
necroptosis
their
roles
provide
new
perspective
for
targeted
Язык: Английский