Surface Molecular Markers for the Isolation of Viable Fibroblast Subpopulations in the Female Reproductive Tract: A Comprehensive Review DOI Open Access
Krzysztof Łuszczyński, Michał Komorowski, Marta Soszyńska

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 233 - 233

Опубликована: Дек. 30, 2024

Advancements in single-cell analyzis technologies, particularly RNA sequencing (scRNA-seq) and Fluorescence-Activated Cell Sorting (FACS), have enabled the of cellular diversity by providing resolutions that were not available previously. These methods enable simultaneous thousands individual transcriptomes, facilitating classification cells into distinct subpopulations, based on transcriptomic differences, adding a new level complexity to biomolecular medical research. Fibroblasts, despite being one most abundant cell types human body forming structural backbone tissues organs, remained poorly characterized for long time. This is largely due high morphological similarity between different fibroblasts lack specific markers identify subpopulations. Once thought be responsible solely synthesis extracellular matrix (ECM) components, are now recognized as active participants diverse physiological processes, including inflammation antimicrobial responses. However, defining molecular profile fibroblast subpopulations remains significant challenge. In this comprehensive review, which over two thousand research articles, we focus identification characterization their surface markers, with an emphasis potential targets selective isolation. By analyzing alongside intra- protein profiles, identified multiple subtypes within female reproductive system. exhibit signatures functional attributes, shaped anatomical localization surrounding or pathological conditions. Our findings underscore heterogeneity roles various biological contexts. improved understanding paves way innovative diagnostic therapeutic strategies, offering precision targeting subsets clinical applications.

Язык: Английский

Nanomaterials in cancer immunotherapy: targeting cancer-associated fibroblasts DOI Creative Commons

Zhongsong Zhang,

Long Chen

Cancer Nanotechnology, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 17, 2025

Emphasizing the significance of cancer-associated fibroblasts (CAFs), non-malignant yet pivotal players within tumor microenvironment (TME), this review illuminates role inflammatory subtype (iCAF) as catalysts in cancer proliferation, metastasis, and therapeutic resistance. Given their paramount importance, targeting CAFs emerges a robust strategy evolving landscape immunotherapy. Nanomaterials, distinguished by unique features malleability, hold considerable promise biomedicine, especially precision-oriented domain therapy. Their aptitude for modulating immune responses, amplifying drug efficacy through precise delivery, discerningly focusing on cells TME situates nanomaterials formidable tools to transcend boundaries set conventional treatments. This scrutinizes convoluted interplay among CAFs, cells, TME. It further showcases widely utilized management. We underscore potential nanoscale delivery systems directed at underscoring transformative power revolutionizing therapies, enhancing precision, culminating improved patient outcomes.

Язык: Английский

Процитировано

2
Association between cancer-associated fibroblasts and prognosis of neoadjuvant chemoradiotherapy in esophageal squamous cell carcinoma: a bioinformatics analysis based on single-cell RNA sequencing DOI Creative Commons
Zhao Huang,

Zhuang‐Zhuang Cong,

Jing Luo

и другие.

Cancer Cell International, Год журнала: 2025, Номер 25(1)

Опубликована: Март 1, 2025

Esophageal squamous cell carcinoma (ESCC) is a prevalent and aggressive subtype of esophageal cancer, posing significant mortality economic burden, especially in East Southeast Asia. Current therapeutic strategies have limitations improving patient survival, particularly regarding disease progression resistance. This study aimed to investigate the impact neoadjuvant chemoradiotherapy (NCRT) on ESCC microenvironment. We utilized single-cell RNA sequencing systematically characterize tumor cancer-associated fibroblasts (CAFs) subtypes. Marker genes myofibroblastic CAFs (myCAFs) were employed establish prognostic model verify its application other datasets. Other experiments conducted clinical samples explore potential risk-related genes. Our bioinformatics statistical analyses revealed an increased proportion epithelial cells NCRT identified Ep_c1 associated with better prognosis. Further results indicated complex communication network between myCAFs. The top 30 marker myCAFs used construct signature response immunotherapy. Finally, Complement C1s subcomponent (C1S), Decorin (DCN), Neuroblastoma suppression tumorigenicity 1 (NBL1) as findings highlight dynamic alterations post-NCRT microenvironment provide foundation for development personalized treatment immunotherapeutic approaches. Future studies are warranted further validate these their implications.

Язык: Английский

Процитировано

0
Advances in nanotechnology for targeting cancer-associated fibroblasts: A review of multi-strategy drug delivery and preclinical insights DOI Creative Commons

Zhongsong Zhang,

Yujie Tang,

Dan Luo

и другие.

APL Bioengineering, Год журнала: 2025, Номер 9(1)

Опубликована: Март 1, 2025

Cancer-associated fibroblasts (CAFs) play a crucial role in the tumor microenvironment by promoting growth, immune evasion, and metastasis. Recently, drug delivery systems targeting CAFs have emerged as promising long-term effective approach to cancer treatment. Advances nanotechnology, particular, led development of nanomedicine designed specifically target CAFs, offering new possibilities for precise personalized therapies. This article reviews recent progress using nanocarriers that CAFs. Additionally, we explore potential combining multiple therapies, such chemotherapy immunotherapy, with enhance efficacy overcome resistance. Although many preclinical studies show promise, clinical application still faces considerable challenges, especially terms penetration large-scale production. Therefore, this review aims provide fresh perspective on CAF-targeted highlight future research directions applications.

Язык: Английский

Процитировано

0
CAFs activated by YAP1 upregulate cancer matrix stiffness to mediate hepatocellular carcinoma progression DOI Creative Commons
Wei Yan, Guohui Xiao, Lijuan Wang

и другие.

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 16, 2025

The stiffness of the matrix is closely related to progression hepatocellular carcinoma (HCC). Although direct targeting stromal rigidity in HCC remains a clinical challenge, cancer-associated fibroblasts (CAFs) are considered key contributors this process. Given heterogeneity CAFs, study explored relationship between specific CAF subsets and liver cancer stiffness, aiming identify novel therapeutic targets for patients. Single-cell sequencing datasets were leveraged cell types within characterize transcriptomic profiles CAFs. Prognostic analysis, utilizing Gene Expression Profiling Interactive Analysis (GEPIA) Cancer Genome Atlas (TCGA) datasets, assessed correlation stiffness-related genes patient outcomes. Pseudo-time analysis was applied trace developmental trajectories By calculating intercellular communication probabilities analyzing transcription factor activity, functions interactions different elucidated. Ontology (GO) used explore functional roles CAFs distinct Yes-associated protein 1 (YAP1) groups. Finally, cellular experiments animal further conducted validate hypotheses study. This identified subpopulations based on single-cell data analyzed transcriptional changes these subpopulations. Key findings include identification collagen type I alpha (COL1A1), III (COL3A1), lysyloxidase (LOX) as pivotal node during development. Moreover, expression inversely correlated with prognosis Notably, YAP1-positive subpopulation emerged primary contributor cancer. upregulates promotes tumor by activating signaling pathways such autophagy GTPase activity regulation. Cellular studies validated conclusion. uncovered subpopulation, particular, shown contribute upregulating relevant promoting through activation pathways.

Язык: Английский

Процитировано

0
Revisiting the role of cancer-associated fibroblasts in tumor microenvironment DOI Creative Commons

Xiaolei Lan,

Wenyang Li, Kai Zhao

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 17, 2025

Cancer-associated fibroblasts (CAFs) are integral components of the tumor microenvironment playing key roles in progression, metastasis, and therapeutic resistance. However, challenges persist understanding their heterogeneity, origin, functional diversity. One major obstacle is lack standardized naming conventions for CAF subpopulations, with current systems failing to capture full complexity. Additionally, identification CAFs hindered by absence specific biomarkers, limiting precision diagnostic strategies. In vitro culture conditions often fail maintain vivo characteristics CAFs, which complicates study translation findings clinical practice. Although detection methods, such as antibodies, mRNA probes, single-cell transcriptomics, offer insights into biology, they standardization provide reliable quantitative measures. Furthermore, dynamic interactions between cells, immune cells within TME remain insufficiently understood, role evasion therapy resistance an area ongoing research. Understanding how influence drug response essential developing more effective cancer therapies. This review aims in-depth analysis research, propose future research directions, emphasize need improved CAF-targeted By addressing these gaps, it seeks highlight potential targets overcoming enhancing efficacy treatments.

Язык: Английский

Процитировано

0
Surface Molecular Markers for the Isolation of Viable Fibroblast Subpopulations in the Female Reproductive Tract: A Comprehensive Review DOI Open Access
Krzysztof Łuszczyński, Michał Komorowski, Marta Soszyńska

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 233 - 233

Опубликована: Дек. 30, 2024

Advancements in single-cell analyzis technologies, particularly RNA sequencing (scRNA-seq) and Fluorescence-Activated Cell Sorting (FACS), have enabled the of cellular diversity by providing resolutions that were not available previously. These methods enable simultaneous thousands individual transcriptomes, facilitating classification cells into distinct subpopulations, based on transcriptomic differences, adding a new level complexity to biomolecular medical research. Fibroblasts, despite being one most abundant cell types human body forming structural backbone tissues organs, remained poorly characterized for long time. This is largely due high morphological similarity between different fibroblasts lack specific markers identify subpopulations. Once thought be responsible solely synthesis extracellular matrix (ECM) components, are now recognized as active participants diverse physiological processes, including inflammation antimicrobial responses. However, defining molecular profile fibroblast subpopulations remains significant challenge. In this comprehensive review, which over two thousand research articles, we focus identification characterization their surface markers, with an emphasis potential targets selective isolation. By analyzing alongside intra- protein profiles, identified multiple subtypes within female reproductive system. exhibit signatures functional attributes, shaped anatomical localization surrounding or pathological conditions. Our findings underscore heterogeneity roles various biological contexts. improved understanding paves way innovative diagnostic therapeutic strategies, offering precision targeting subsets clinical applications.

Язык: Английский

Процитировано

0