Integrated lncRNA and mRNA analysis reveals the immune modulatory mechanisms of antimicrobial peptide BSN-37 in mouse peritoneal macrophages DOI Creative Commons
Huihui Zhang,

Yanhe Lv,

Jingjing Li

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Июнь 2, 2025

Antimicrobial peptides (AMPs) possess vaccine adjuvant activity; however, their specific targets and molecular mechanisms remain incompletely understood, which hinders clinical application. This study aimed to elucidate the key pathways through antimicrobial peptide BSN-37 modulates immune responses in macrophages, providing evidence for its potential translation. In this investigation, Balb/c mice were administered 12 h, after total RNA was extracted from peritoneal macrophages assess mRNA expression levels of cytokines molecules on cell surface, followed by transcriptomic sequencing. The results demonstrated that significantly upregulated these cytokines. A 228 differentially expressed long non-coding RNAs (lncRNAs) (121 upregulated, 107 downregulated) 149 mRNAs (104 45 identified. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway analyses revealed significant enrichment response pathways, PI3K-Akt signaling, NOD-like receptor signaling. Differentially lncRNA target genes associated with T PD-1 checkpoint regulation, other regulatory pathways. Protein-protein interaction network analysis identified core such as CCchemokine 1 (CCR1) Toll Like Receptor 8 (TLR8). Molecular docking studies confirmed exhibited strong binding affinity TLR8 CCR1, energies less than - 5 kcal/mol. RT-qPCR validation reliability sequencing data. These findings indicate activates multiple targeting immune-related offering theoretical support development novel adjuvants.

Язык: Английский

Integrated lncRNA and mRNA analysis reveals the immune modulatory mechanisms of antimicrobial peptide BSN-37 in mouse peritoneal macrophages DOI Creative Commons
Huihui Zhang,

Yanhe Lv,

Jingjing Li

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Июнь 2, 2025

Antimicrobial peptides (AMPs) possess vaccine adjuvant activity; however, their specific targets and molecular mechanisms remain incompletely understood, which hinders clinical application. This study aimed to elucidate the key pathways through antimicrobial peptide BSN-37 modulates immune responses in macrophages, providing evidence for its potential translation. In this investigation, Balb/c mice were administered 12 h, after total RNA was extracted from peritoneal macrophages assess mRNA expression levels of cytokines molecules on cell surface, followed by transcriptomic sequencing. The results demonstrated that significantly upregulated these cytokines. A 228 differentially expressed long non-coding RNAs (lncRNAs) (121 upregulated, 107 downregulated) 149 mRNAs (104 45 identified. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway analyses revealed significant enrichment response pathways, PI3K-Akt signaling, NOD-like receptor signaling. Differentially lncRNA target genes associated with T PD-1 checkpoint regulation, other regulatory pathways. Protein-protein interaction network analysis identified core such as CCchemokine 1 (CCR1) Toll Like Receptor 8 (TLR8). Molecular docking studies confirmed exhibited strong binding affinity TLR8 CCR1, energies less than - 5 kcal/mol. RT-qPCR validation reliability sequencing data. These findings indicate activates multiple targeting immune-related offering theoretical support development novel adjuvants.

Язык: Английский

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