Influence of acetylation and carboxymethylation on physicochemical characteristics of glucan exopolysaccharide isolated from Enterococcus hirae OL616073
Irshad Ahmad Shah,
Swati Tiwari,
Palanisamy Bruntha Devi
и другие.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Май 6, 2025
Abstract
Chemical
modifications
such
as
acetylation
and
carboxymethylation
significantly
influence
the
physicochemical
properties
of
biopolymers,
including
exopolysaccharides
(EPS).
In
this
study,
glucan
EPS
from
Enterococcus
hirae
OL616073
was
chemically
modified
to
acetylated
(AG)
carboxymethylated
(CG)
via
an
affordable
process.
The
structure
were
investigated
by
1H
NMR
spectroscopy,
Fourier
transform
infrared
(FTIR),
scanning
electron
microscope
(SEM),
contact
angle,
particle
size,
thermal
analysis
(TGA-DSC),
X-ray
diffractometer
(XRD).
results
demonstrated
effect
modification
on
its
properties.
As
L*
varied
between
70.13
74.46
with
a
mild
darkening
in
derivative
than
carboxymethylated,
a*
ranged
3.55–3.87,
affirming
reddish
tint
all
powders.
There
slight
increase
(70.24)
whiteness
index
(WI)
for
CG
comparison
AG
(68.10)
Untreated
(UT)
(65.23).
Decrease
bulk
density
(0.532
0.415
g/mL),
tapped
(0.756
0.531g/mL),
Hausner
ratio
(1.42
1.27),
compressibility
(CI)
(29.61
21.81%)
UT
observed.
Micrographs
revealed
significant
change
agglomerated
granules
AC
rough
collapsed
CG.
Water
activity
(a
w)
untreated
decreased
0.358
0.345
further
increased
0.380
size
(226.6
nm)
365.8
nm
526.4
Acetylation
enhanced
hydrophobicity
(water
angle:
56.58°
66.28°),
while
became
more
hydrophilic
(44.13°).
holding
capacity
328.11%
494.22%
(CG).
emulsion
stability
retained
better
at
different
time
intervals.
Furthermore,
showed
antitumor
effects
against
HCT116
cells
(8.18
47.04%
reduction)
(2.04
17.22%)
25–500
µg/mL.
findings
highlight
functional
groups
reaction
conditions
properties,
suggesting
their
potential
food
development.
Язык: Английский
Optimising the production of exopolysaccharides from Pediococcus pentosaceus SSC-12 and characterizing its properties
LWT,
Год журнала:
2025,
Номер
unknown, С. 117967 - 117967
Опубликована: Май 1, 2025
Язык: Английский