Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Окт. 28, 2024
Idiopathic
Nephrotic
Syndrome
(INS)
is
a
common
childhood
glomerular
disease
requiring
intense
immunosuppressive
drug
treatments.
Prediction
of
treatment
response
and
the
occurrence
relapses
remains
challenging.
Biofluid-derived
extracellular
vesicles
(EVs)
may
serve
as
novel
liquid
biopsies
for
INS
classification
monitoring.
Our
cohort
was
composed
105
children
at
different
clinical
time
points
(onset,
relapse,
persistent
proteinuria,
remission,
respectively),
19
healthy
controls.
The
expression
37
surface
EV
markers
evaluated
by
flow
cytometry
in
serum
(n
=
83)
urine
74)
from
(mean
age
10.1,
58%
males)
points.
Urine
EVs
7)
11)
age-matched
7.8,
94%
were
also
analyzed.
Tetraspanin
enhanced
during
active
phase
respect
to
remission
group
positively
correlates
with
proteinuria
levels.
Unsupervised
clustering
analysis
identified
an
signature
8
related
immunity
angiogenesis/adhesion
processes.
CD41b,
CD29,
CD105
showed
best
diagnostic
scores
separating
condition.
Interestingly,
combining
urinary
same
patient
improved
precision
staging
separation.
Three
biomarkers
(CD19,
CD44,
CD8)
able
classify
based
on
steroid
sensitivity.
Biofluid
offer
non-invasive
tool
subclassification
"personalized"
interventions.
Pharmaceuticals,
Год журнала:
2024,
Номер
17(3), С. 329 - 329
Опубликована: Март 1, 2024
In
recent
years,
due
to
their
distinctive
and
adaptable
therapeutic
effects,
many
natural
bioactive
compounds
have
been
commonly
used
treat
diseases.
Their
limited
solubility,
low
bioavailability,
inadequate
gastrointestinal
tract
stability,
high
metabolic
rate,
shorter
duration
of
action
pharmaceutical
applications.
However,
those
can
be
improved
using
nanotechnology
create
various
drug
delivery
systems,
including
lipid-based
nanoparticles,
adjust
the
compounds’
physicochemical
properties
pharmacokinetic
profile.
Because
enormous
technical
advancements
made
in
fundamental
sciences
physical
chemical
manipulation
individual
atoms
molecules,
subject
has
experienced
revolutionary
growth.
By
fabricating
certain
functionalized
particles,
opens
an
innovative
horizon
research
development
for
overcoming
restrictions,
traditional
medication
administration
systems.
Nanotechnology-driven
are
a
impact
clinical
value
current
future
uses.
Lipid-based
nanotechnologies
were
shown
deliver
range
naturally
occurring
with
decent
entrapment
potential
successfully
controlled
release,
increased
intriguing
activity.
This
review
outlines
such
as
paclitaxel,
curcumin,
rhodomyrtone,
quercetin,
kaempferol,
resveratrol,
epigallocatechin-3-gallate,
silymarin,
oridonin,
fortified
within
either
or
synthetic
system
based
on
evaluation
considerations.
Pharmaceutics,
Год журнала:
2025,
Номер
17(2), С. 264 - 264
Опубликована: Фев. 17, 2025
Background
and
Aim:
Biliary
atresia
is
a
rare,
progressive
disease
that
affects
the
bile
ducts
in
newborns.
Persistent
duct
obstruction
induces
various
pathological
conditions,
including
jaundice,
inflammation,
liver
fibrosis;
however,
exact
pathogenesis
of
biliary
not
yet
fully
understood.
Nuclear
factor-κB
(NF-κB)
widely
acknowledged
as
key
regulator
hepatitis
fibrosis,
extensive
research
has
been
conducted
to
develop
strategies
effectively
inhibit
its
activity
mitigate
damage.
Exosome-based
therapeutic
platforms
offer
targeted
NF-κB
inhibition
with
low
immunogenicity
enhanced
liver-specific
delivery.
This
study
aimed
evaluate
efficacy
Exo-SrIκB
treating
cholestatic
fibrosis
using
experimental
animal
models.
Methods:
(an
exosome-based
therapy
containing
super-repressor
IκB
protein)
EXPLOR
technology
(Exosome
engineering
for
Protein
Loading
via
Optically
Reversible
protein-protein
interactions)
encapsulate
super
repressor
(SrIκB)
within
exosomes.
The
was
assessed
minipig
mouse
models
experimentally
induced
disease.
Results:
Administration
significantly
attenuated
progression
both
by
inhibiting
nuclear
translocation
reducing
expression
fibrotic
markers.
Treated
animals
exhibited
reduced
collagen
deposition,
lower
α-SMA
levels,
improved
hepatic
function
compared
untreated
controls.
Conclusion:
suppressed
signaling
alleviated
models,
suggesting
therapeutics
may
biocompatible
application
managing
other
chronic
diseases.
Biomaterials Research,
Год журнала:
2025,
Номер
29
Опубликована: Янв. 1, 2025
The
repair
of
peripheral
nerve
injury
(PNI)
presents
a
multifaceted
and
protracted
challenge,
with
current
therapeutic
approaches
failing
to
achieve
optimal
outcomes,
thereby
not
satisfying
the
considerable
clinical
demand.
advent
tissue
engineering
has
led
growing
body
experimental
evidence
indicating
that
synergistic
application
conduits,
which
provide
structural
guidance,
alongside
biological
signals
derived
from
exosomes
stem
cells,
yields
superior
results
for
PNI
compared
isolated
interventions.
This
combined
approach
holds
great
promise
application.
In
this
review,
we
present
latest
advancements
in
treatment
through
integration
cells
or
conduits.
We
have
addressed
inadequate
efficiency
conjunction
conduits
3
perspectives:
enhancing
exosomes,
improving
incorporating
physical
stimulation.
Proteomes,
Год журнала:
2025,
Номер
13(1), С. 12 - 12
Опубликована: Март 4, 2025
Extracellular
vesicles
(EVs)
are
lipid-bound
secreted
by
cells,
including
exosomes,
microvesicles,
and
apoptotic
bodies.
Proteomic
analyses
of
EVs,
particularly
in
relation
to
cancer,
reveal
specific
biomarkers
crucial
for
diagnosis
therapy.
However,
isolation
techniques
such
as
ultracentrifugation,
size-exclusion
chromatography,
ultrafiltration
face
challenges
regarding
purity,
contamination,
yield.
Contamination
from
other
proteins
complicates
downstream
processing,
leading
difficulties
identifying
interpreting
results.
Future
research
will
focus
on
refining
EV
characterization
diagnostic
therapeutic
applications,
improving
proteomics
tools
greater
accuracy,
exploring
the
use
EVs
drug
delivery
regenerative
medicine.
In
this
review,
we
provide
a
bird’s
eye
view
various
challenges,
starting
with
methods,
yield,
limitations
proteome
analysis
protein
targets.
