Uncovering immune cell heterogeneity in hepatocellular carcinoma by combining single-cell RNA sequencing with T-cell receptor sequencing DOI
Xinyu Gu, Siyu Gu, Ziyi Chen

и другие.

World Journal of Hepatology, Год журнала: 2025, Номер 17(2)

Опубликована: Фев. 20, 2025

BACKGROUND Understanding the status and function of tumor-infiltrating immune cells is essential for improving immunotherapeutic effects predicting clinical response in human patients with carcinoma. However, little known about cells, corresponding research results hepatocellular carcinoma (HCC) are limited. AIM To investigate potential biomarker genes that important development HCC to understand how cell subsets react throughout this process. METHODS Using single-cell RNA sequencing T-cell receptor sequencing, heterogeneity functions subpopulations from tissue normal adjacent carcinoma, as well their possible interactions, were analyzed. RESULTS Eight clusters analyzed identified using bioinformatics, including six typical major two newly clusters, among which Fc epsilon 1G+ T characterized by upregulation 1G, tyrosine kinase binding protein, delta constant, whereas metallothionein 1E+ proliferated significantly tumors. Differentially expressed genes, such regulator cycle, cysteine serine rich nuclear protein 1, SMAD7 1E, upregulated tumors have biomarkers. In association analysis, we inferred clonal expansion characteristics each cluster patients. CONCLUSION We lymphocyte critical revealed amplification infiltrating cells. These data provide valuable resources understanding HCC.

Язык: Английский

Fibroblasts in cancer: Unity in heterogeneity DOI Creative Commons
Yash Chhabra, Ashani T. Weeraratna

Cell, Год журнала: 2023, Номер 186(8), С. 1580 - 1609

Опубликована: Апрель 1, 2023

Язык: Английский

Процитировано

213
Gallbladder cancer DOI
Juan Carlos Roa, Patricia García, Vinay K. Kapoor

и другие.

Nature Reviews Disease Primers, Год журнала: 2022, Номер 8(1)

Опубликована: Окт. 27, 2022

Язык: Английский

Процитировано

202
Heterogeneity and plasticity of epithelial–mesenchymal transition (EMT) in cancer metastasis: Focusing on partial EMT and regulatory mechanisms DOI Creative Commons

Dandan Li,

Lingyun Xia,

Pan Huang

и другие.

Cell Proliferation, Год журнала: 2023, Номер 56(6)

Опубликована: Фев. 19, 2023

Epithelial-mesenchymal transition (EMT) or mesenchymal-epithelial (MET) plays critical roles in cancer metastasis. Recent studies, especially those based on single-cell sequencing, have revealed that EMT is not a binary process, but heterogeneous and dynamic disposition with intermediary partial states. Multiple double-negative feedback loops involved by EMT-related transcription factors (EMT-TFs) been identified. These between drivers MET finely regulate the state of cell. In this review, general characteristics, biomarkers molecular mechanisms different states were summarized. We additionally discussed direct indirect tumour More importantly, article provides evidence heterogeneity closely related to poor prognosis gastric cancer. Notably, seesaw model was proposed explain how cells themselves remain specific states, including epithelial state, hybrid/intermediate mesenchymal state. Additionally, also review current status, limitations future perspectives signalling clinical applications.

Язык: Английский

Процитировано

80
Intratumour oxidative hotspots provide a niche for cancer cell dissemination DOI
Yoshifumi Ueda, Shigeki Kiyonaka, Laura M. Selfors

и другие.

Nature Cell Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 21, 2025

Язык: Английский

Процитировано

2
Epithelial–Mesenchymal Plasticity in Tumor Immune Evasion DOI Open Access
Riley Mullins, Ananya Pal, Thomas F. Barrett

и другие.

Cancer Research, Год журнала: 2022, Номер 82(13), С. 2329 - 2343

Опубликована: Апрель 1, 2022

Epithelial-mesenchymal transition (EMT) is a fundamental process that occurs during embryogenesis and tissue repair. However, EMT can be hijacked by malignant cells, where it may promote immune evasion metastasis. Classically considered dichotomous transition, in cancer has recently been plastic whereby cells display interconvert among hybrid epithelial/mesenchymal (E/M) states. plasticity (EMP) associated E/M states are divergent from classical EMT, with unique immunomodulatory effects. Here, we review recent insights into the EMP-immune cross-talk, highlighting possible mechanisms of conferred roles EMP.

Язык: Английский

Процитировано

40
Clinical impact of epithelial–mesenchymal transition for cancer therapy DOI Creative Commons
Nobendu Mukerjee, Sagnik Nag, Bikramjit Bhattacharya

и другие.

Clinical and Translational Discovery, Год журнала: 2024, Номер 4(1)

Опубликована: Янв. 28, 2024

Abstract The epithelial–mesenchymal transition (EMT) represents a pivotal frontier in oncology, playing central role the metastatic cascade of cancer—a leading global health challenge. This comprehensive review delves into complexities EMT, process where cancer cells gain exceptional mobility, facilitating their invasion distant organs and establishment secondary malignancies. We thoroughly examine myriad factors influencing encompassing transcription factors, signalling pathways, metabolic alterations, microRNAs, long non‐coding RNAs, epigenetic changes, exosomal interactions intricate dynamics tumour microenvironment. Particularly, emphasises advanced stages crucial for development highly aggressive phenotypes. During this phase, penetrate vascular barrier exploit bloodstream to propagate life‐threatening metastases through mesenchymal–epithelial transition. also explore EMT's significant fostering dormancy, senescence, emergence stem formidable challenge therapeutic resistance. Our transcends mere inventory EMT‐inducing elements; it critically assesses current state EMT‐focused clinical trials, revealing both hurdles breakthroughs. Highlighting potential EMT research, we project its transformative impact on future therapy. exploration is aimed at paving way towards an era effectively managing relentless disease, positioning forefront innovative research strategies.

Язык: Английский

Процитировано

11
The Inflammatory Profile of the Tumor Microenvironment, Orchestrated by Cyclooxygenase-2, Promotes Epithelial-Mesenchymal Transition DOI Creative Commons
Fernán Gómez‐Valenzuela, Enrico Escobar, Ricardo Pérez‐Tomás

и другие.

Frontiers in Oncology, Год журнала: 2021, Номер 11

Опубликована: Июнь 10, 2021

The tumor microenvironment (TME) corresponds to a complex and dynamic interconnection between the extracellular matrix malignant cells their surrounding stroma composed of immune mesenchymal cells. TME has constant cellular communication through cytokines that sustain an inflammatory profile, which favors progression, angiogenesis, cell invasion, metastasis. Although epithelial-mesenchymal transition (EMT) represents relevant metastasis-initiating event promotes invasive phenotype in epithelial cells, its relationship with profile is poorly understood. Previous evidence strongly suggests cyclooxygenase-2 (COX-2) overexpression, pro-inflammatory enzyme related chronic unresolved inflammation, associated common EMT-signaling pathways. This review article summarizes how COX-2 within context TME, orchestrates EMT process initial metastatic-related events.

Язык: Английский

Процитировано

51
Research progress on the anti-tumor effect of Naringin DOI Creative Commons

Jing He,

Huiping Zhang

Frontiers in Pharmacology, Год журнала: 2023, Номер 14

Опубликована: Авг. 17, 2023

Naringin is a kind of natural dihydro flavone, which mainly exists in citrus fruits the Rutaceae family, as well traditional Chinese medicines such trifoliate orange, fingered citron, exocarpium citri grandis, and rhizoma dynamite. Modern pharmacological studies have shown that has excellent anti-tumor activity. Through reviewing relevant literature at home abroad recent years, we summarized mechanism to play an anti-cancer role blocking tumor cell cycle, inhibiting proliferation, inducing apoptosis, invasion metastasis, autophagy, reversing drug resistance enhancing chemotherapeutic sensitivity, anti-inflammatory prevent canceration, alleviate Adverse reaction chemotherapy, activate strengthen immunity, It provides theoretical basis reference for further exploring anticancer potential its development utilization.

Язык: Английский

Процитировано

19
VC-resist glioblastoma cell state: vessel co-option as a key driver of chemoradiation resistance DOI Creative Commons

Cathy Pichol-Thievend,

Océane Anezo,

Aafrin M. Pettiwala

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 29, 2024

Abstract Glioblastoma (GBM) is a highly lethal type of cancer. GBM recurrence following chemoradiation typically attributed to the regrowth invasive and resistant cells. Therefore, there pressing need gain deeper understanding mechanisms underlying resistance its ability infiltrate. Using combination transcriptomic, proteomic, phosphoproteomic analyses, longitudinal imaging, organotypic cultures, functional assays, animal studies, clinical data we demonstrate that brain vasculature induce cell transition state named VC-Resist (vessel co-opting state). This midway along transcriptomic axis between proneural mesenchymal cells closer AC/MES1-like state. are vessel co-opting, allowing significant infiltration into surrounding tissue homing perivascular niche, which in turn induces even more transition. The molecular characteristics this FGFR1-YAP1-dependent state, including DNA damage, enrichment G2M phase, induction senescence/stemness pathways, contribute enhanced chemoradiation. These findings how co-option, plasticity jointly drive therapy during recurrence.

Язык: Английский

Процитировано

8
IL-1β-activated PI3K/AKT and MEK/ERK pathways coordinately promote induction of partial epithelial–mesenchymal transition DOI Creative Commons
Yosuke Tabei, Yoshihiro Nakajima

Cell Communication and Signaling, Год журнала: 2024, Номер 22(1)

Опубликована: Авг. 8, 2024

Abstract Epithelial–mesenchymal transition (EMT) is a cellular process in embryonic development, wound healing, organ fibrosis, and cancer metastasis. Previously, we others have reported that proinflammatory cytokine interleukin-1β (IL-1β) induces EMT. However, the exact mechanisms, especially signal transduction pathways, underlying IL-1β-mediated EMT are not yet completely understood. Here, found IL-1β stimulation leads to partial EMT-like phenotype human lung epithelial A549 cells, including gain of mesenchymal marker (vimentin) high migratory potential, without complete loss (E-cadherin). induction was repressed by PI3K inhibitor LY294002, indicating PI3K/AKT pathway plays significant role induction. In addition, ERK1/2 FR180204 markedly inhibited induction, demonstrating MEK/ERK also involved Furthermore, activation pathways occurred downstream epidermal growth factor receptor (EGFR) IL-1 (IL-1R) pathway, respectively. Our findings suggest coordinately promote The inhibition one but both expected yield clinical benefits preventing EMT-related disorders such as fibrosis

Язык: Английский

Процитировано

8