Extracellular matrix remodelling in dental pulp tissue of carious human teeth through the prism of single-cell RNA sequencing DOI Creative Commons
Anamaria Balic, Dilara Perver, Pierfrancesco Pagella

и другие.

International Journal of Oral Science, Год журнала: 2023, Номер 15(1)

Опубликована: Авг. 2, 2023

Abstract Carious lesions are bacteria-caused destructions of the mineralised dental tissues, marked by simultaneous activation immune responses and regenerative events within soft pulp tissue. While major molecular players in tooth decay have been uncovered during past years, a detailed map cellular landscape diseased is still missing. In this study we used single-cell RNA sequencing analysis, supplemented with immunostaining, to generate comprehensive atlas carious human teeth. Our data demonstrated modifications various cell clusters teeth, such as cells, mesenchymal stem cells (MSC) fibroblasts, when compared healthy Active response tissue accompanied specific changes fibroblast MSC clusters. These include upregulation genes encoding extracellular matrix (ECM) components, including COL1A1 Fibronectin (FN1) , enrichment cluster myofibroblasts. The incremental ECM composition tissues were further confirmed immunostaining analyses. Assessment fibres under mechanical strain conditions showed significant tension reduction ones. present demonstrate molecular, biomechanical alterations indicative extensive remodelling, reminiscent fibrosis observed other organs. This teeth can facilitate future studies pathologies enable comparative analyses across

Язык: Английский

Different Sources of Mesenchymal Stem Cells for Tissue Regeneration: A Guide to Identifying the Most Favorable One in Orthopedics and Dentistry Applications DOI Open Access
Víctor J. Costela‐Ruiz, Lucia Melguizo‐Rodríguez, Chiara Bellotti

и другие.

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(11), С. 6356 - 6356

Опубликована: Июнь 6, 2022

The success of regenerative medicine in various clinical applications depends on the appropriate selection source mesenchymal stem cells (MSCs). Indeed, conditions, quality and quantity MSCs, have an influence growth factors, cytokines, extracellular vesicles, secrete bioactive factors milieu, thus influencing result. Thus, optimal should harmonize this complex setting ensure a well-personalized effective treatment. Mesenchymal (MSCs) can be obtained from several sources, including bone marrow adipose tissue, already used orthopedic applications. In sense, for bone, dental, oral injuries, MSCs could provide innovative therapy. present review aims to compare properties (proliferation, migration, clonogenicity, angiogenic capacity, differentiation potential, secretome) derived marrow, dental tissue enable clinicians select best their application regeneration delineate new translational perspectives. A literature was conducted using search engines Web Science, Pubmed, Scopus, Google Scholar. An analysis different publications showed that all sources compared (bone (BM-MSCs), (AT-MSCs), (DT-MSCs)) are good options promote proper migration angiogenesis, they turn out useful gingival, pulp, periodontal regeneration. particular, DT-MSCs better proliferation rates AT G-MSC higher clonogenicity. widely medicine, preferable ability. Considering among BM-MSCs, AT-MSCs, as potential candidates

Язык: Английский

Процитировано

85
DLP printed hDPSC-loaded GelMA microsphere regenerates dental pulp and repairs spinal cord DOI
Ying Qian, Jiaxing Gong, Kejie Lu

и другие.

Biomaterials, Год журнала: 2023, Номер 299, С. 122137 - 122137

Опубликована: Апрель 27, 2023

Язык: Английский

Процитировано

45
Spatiotemporal single-cell regulatory atlas reveals neural crest lineage diversification and cellular function during tooth morphogenesis DOI Creative Commons
Junjun Jing, Jifan Feng, Yuan Yuan

и другие.

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Авг. 16, 2022

Cranial neural crest cells are an evolutionary innovation of vertebrates for craniofacial development and function, yet the mechanisms that govern cell fate decisions postmigratory cranial remain largely unknown. Using mouse molar as a model, we perform single-cell transcriptome profiling to interrogate diversification cells. We reveal landscape transcriptional heterogeneity define specific cellular domains during progression cell-derived dental lineage diversification, find each domain makes contribution distinct mesenchymal tissues. Furthermore, IGF signaling-mediated cell-cell interaction between highlights pivotal role autonomous regulation mesenchyme. Importantly, cell-type-specific gene regulatory networks in mesenchyme show Foxp4 is indispensable differentiation periodontal ligament. Our atlas provides comprehensive mechanistic insight into process odontogenic populations.

Язык: Английский

Процитировано

59
Mesenchymal condensation in tooth development and regeneration: a focus on translational aspects of organogenesis DOI
Bing‐Dong Sui,

Chenxi Zheng,

Wanmin Zhao

и другие.

Physiological Reviews, Год журнала: 2023, Номер 103(3), С. 1899 - 1964

Опубликована: Янв. 19, 2023

The teeth are vertebrate-specific, highly specialized organs performing fundamental functions of mastication and speech, the maintenance which is crucial for orofacial homeostasis further linked to systemic health human psychosocial well-being. However, with limited ability self-repair, can often be impaired by traumatic, inflammatory, progressive insults, leading high prevalence tooth loss defects worldwide. Regenerative medicine holds promise achieve physiological restoration lost or damaged organs, in particular an evolving framework developmental engineering has pioneered functional regeneration harnessing odontogenic program. As a key event morphogenesis, mesenchymal condensation dictates dental tissue formation patterning through cellular self-organization signaling interaction epithelium, provides representative decipher organogenetic mechanisms leveraged regenerative purposes. In this review, we summarize how spatiotemporally assembles from stem cells (DSCs) sequentially mediates development. We highlight condensation-mimetic efforts based on ex vivo aggregation DSCs, have achieved functionally robust physiologically relevant after implantation animals humans. discussion aspect will add knowledge development-inspired strategies offer benefits propel clinical organ regeneration.

Язык: Английский

Процитировано

28
Single-cell and spatially resolved interactomics of tooth-associated keratinocytes in periodontitis DOI Creative Commons
Quinn T. Easter, Bruno Fernandes Matuck, G. B. Stark

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июнь 14, 2024

Periodontitis affects billions of people worldwide. To address relationships periodontal niche cell types and microbes in periodontitis, we generated an integrated single-cell RNA sequencing (scRNAseq) atlas human periodontium (34-sample, 105918-cell), including sulcular junctional keratinocytes (SK/JKs). SK/JKs displayed altered differentiation states were enriched for effector cytokines periodontitis. Single-cell metagenomics revealed 37 bacterial species with cell-specific tropism. Fluorescence situ hybridization detected intracellular 16 S mRNA signals multiple correlated SK/JK proinflammatory phenotypes situ. Cell-cell communication analysis predicted keratinocyte-specific innate adaptive immune interactions. Highly multiplexed immunofluorescence (33-antibody) peri-epithelial foci, cells often spatially constrained around JKs. Spatial phenotyping immunosuppressed JK-microniches SK-localized tertiary lymphoid structures Here, demonstrate impacts on interactomics SK JK health which requires further investigation to support precision interventions chronic inflammation.

Язык: Английский

Процитировано

12
Creating an atlas of the bone microenvironment during oral inflammatory-related bone disease using single-cell profiling DOI Creative Commons

Yi Fan,

Ping Lyu, Ruiye Bi

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Фев. 1, 2023

Oral inflammatory diseases such as apical periodontitis are common bacterial infectious that may affect the periapical alveolar bone tissues. A protective process occurs simultaneously with tissue destruction, in which mesenchymal stem cells (MSCs) play a primary role. However, systematic and precise description of cellular molecular composition microenvironment affected by inflammation is lacking. In this study, we created single-cell atlas cell populations compose healthy disease states. We investigated changes expression frequency patterns related to periodontitis, well interactions between MSCs immunocytes. Our results highlight an enhanced self-supporting network osteogenic potential within during periodontitis-associated inflammation. not only differentiated toward osteoblast lineage but also expressed higher levels osteogenic-related markers, including Sparc Col1a1. This was confirmed tracing transgenic mouse models human samples from oral inflammatory-related lesions. summary, current study provides in-depth immunocytes both identified key MSC subclusters their biomarkers, could further our understanding underlying mechanisms disease. Taken together, these enhance heterogeneity under pathogenic conditions. provide data tool for investigators better appreciate repertoire progenitors stress responsive importantly help design new therapeutic targets restore lesions caused other diseases.

Язык: Английский

Процитировано

17
Dental Pulp Stem Cell Heterogeneity: Finding Superior Quality “Needles” in a Dental Pulpal “Haystack” for Regenerative Medicine-Based Applications DOI Creative Commons

Zi Y. Kok,

Nadia Y. A. Alaidaroos,

Amr Alraies

и другие.

Stem Cells International, Год журнала: 2022, Номер 2022, С. 1 - 20

Опубликована: Янв. 4, 2022

Human dental pulp stem/stromal cells (hDPSCs) derived from the permanent secondary dentition are recognised to possess certain advantageous traits, which support their potential use as a viable source of mesenchymal (MSCs) for regenerative medicine-based applications. However, well-established heterogeneous nature hDPSC subpopulations, coupled with limited numbers within tissues, has impeded our understanding biology and translation sufficient quantities these laboratory research, through successful therapy development clinical This article reviews current evidence underpinning molecular basis heterogeneity, may be exploited distinguish individual subpopulations specific or superior characteristics medicine Pertinent unanswered questions still remain, regarding developmental origins, hierarchical organisation, stem cell niche locations roles in heterogeneity functions, will further explored. Ultimately, greater how key features, such surface, senescence other relevant genes, protein metabolic markers, delineate between contrasting stemness, proliferative, multipotency, immunomodulatory, anti-inflammatory, properties is required. Such knowledge advancements undoubtedly lead novel screening, isolation, purification strategies, permitting routine effective identification, enrichment, expansion more desirable Furthermore, innovative measures could improved expansion, manufacture, banking procedures, thereby supporting translational hDPSC-based therapies future.

Язык: Английский

Процитировано

27
Novel approaches for periodontal tissue engineering DOI Creative Commons
W. Benton Swanson, Yao Yao, Yuji Mishina

и другие.

genesis, Год журнала: 2022, Номер 60(8-9)

Опубликована: Сен. 1, 2022

Summary The periodontal complex involves the hard and soft tissues which support dentition, comprised of cementum, bone, ligament (PDL). Periodontitis, a prevalent infectious disease periodontium, threatens integrity these causes irreversible damage. Periodontal therapy aims to repair ultimately regenerate toward preserving native dentition improving physiologic integration dental implants. PDL contains multipotent stem cells, have robust capacity differentiate into various types cells form PDL, alveolar bone. Selection appropriate growth factors biomaterial matrices facilitate regeneration are critical recapitulate organization function complex. Herein, we discuss current state clinical including review FDA‐approved factors. We will highlight advances in preclinical research identifying additional capable augment similarly synergistically, regeneration's predictability long‐term efficacy. This should improve readers' understanding molecular cellular processes involving essential for designing comprehensive therapeutic approaches.

Язык: Английский

Процитировано

26
Periodontal tissue stem cells and mesenchymal stem cells in the periodontal ligament DOI Creative Commons
Tomoaki Iwayama,

Hiromi Sakashita,

Masahide Takedachi

и другие.

Japanese Dental Science Review, Год журнала: 2022, Номер 58, С. 172 - 178

Опубликована: Май 17, 2022

Periodontal tissue stem cells, which play a crucial role in maintaining the homeostasis of periodontal tissues, are found ligament (PDL). These cells have long been referred to as mesenchymal stem/stromal (MSCs), and their clinical applications extensively studied. However, PDL not thoroughly investigated, they may be different from MSCs. Recent advances cell biology, such genetic lineage tracing, identification label-retaining single-cell transcriptome analysis, made it possible analyze vivo. In this review, we summarize recent findings on these populations discuss future research directions toward developing regenerative therapy.

Язык: Английский

Процитировано

25
Single-cell integration reveals metaplasia in inflammatory gut diseases DOI Creative Commons
Amanda J. Oliver, Ni Huang, Raquel Bartolomé-Casado

и другие.

Nature, Год журнала: 2024, Номер 635(8039), С. 699 - 707

Опубликована: Ноя. 20, 2024

Abstract The gastrointestinal tract is a multi-organ system crucial for efficient nutrient uptake and barrier immunity. Advances in genomics surge diseases 1,2 has fuelled efforts to catalogue cells constituting tissues health disease 3 . Here we present systematic integration of 25 single-cell RNA sequencing datasets spanning the entire healthy development adulthood. We uniformly processed 385 samples from 189 controls using newly developed automated quality control approach (scAutoQC), leading reference atlas with approximately 1.1 million 136 fine-grained cell states. anchor 12 cancers, coeliac disease, ulcerative colitis Crohn’s this reference. Utilizing 1.6 resource (gutcellatlas.org), discover epithelial metaplasia originating stem intestinal inflammatory transcriptional similarity found pyloric Brunner’s glands. Although previously linked mucosal healing 4 , now implicate gland metaplastic inflammation through recruitment immune including T neutrophils. Overall, describe inflammation-induced changes that alter tissue architecture promote further inflammation, concept applicable other diseases.

Язык: Английский

Процитировано

6