Cell Replacement Therapy for Brain Repair: Recent Progress and Remaining Challenges for Treating Parkinson’s Disease and Cortical Injury

Brain Sciences, Год журнала: 2023, Номер 13(12), С. 1654 - 1654

Опубликована: Ноя. 29, 2023

Neural transplantation represents a promising approach to repairing damaged brain circuitry. Cellular grafts have been shown promote functional recovery through “bystander effects” and other indirect mechanisms. However, extensive lesions may require direct neuronal replacement achieve meaningful restoration of function. While fetal cortical integrate with the host appear develop appropriate attributes, significant ethical concerns limited availability this tissue severely hamper clinical translation. Induced pluripotent stem cell-derived cells tissues represent more readily scalable alternative. Significant progress has recently made in developing protocols for generating wide range neural cell types vitro. Here, we discuss recent approaches two conditions distinct design needs: Parkinson’s disease injury. We current status future application injections dopaminergic treatment as well use structured such organoids repair.

Язык: Английский

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Libertellenone C attenuates oxidative stress and neuroinflammation with the capacity of NLRP3 inhibition

Natural Products and Bioprospecting, Год журнала: 2024, Номер 14(1)

Опубликована: Фев. 26, 2024

Neurodegenerative diseases (NDs) are common chronic arising from progressive damage to the nervous system. Here, in-house natural product database screening revealed that libertellenone C (LC) obtained fermentation products of Arthrinium arundinis separated gut a centipede collected in our Tongji campus, showed remarkable neuroprotective effect. Further investigation was conducted clarify specific mechanism. LC dose-dependently reversed glutamate-induced decreased viability, accumulated reactive oxygen species, mitochondrial membrane potential loss, and apoptosis SH-SY5Y cells. Network pharmacology analysis predicted targets were most likely directly related oxidative stress regulation inflammatory factor-associated signaling pathways. study demonstrated attenuated nitrite, TNF-α, IL-1β production inducible nitric oxide synthase cyclooxygenase expression lipopolysaccharide-induced BV-2 could inhibit NLRP3 inflammasome activation by decreasing levels NLRP3, ASC, cleaved Caspase-1, NF-κB p65. Our results provide new understanding how inhibits microglia, providing neuroprotection. These findings might guide development effective LC-based therapeutic strategies for NDs.

Язык: Английский

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Human brain organoids containing microglia that have arisen innately adapt to a β-amyloid challenge better than those in which microglia are integrated by co-culture

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 13, 2024

Alzheimer disease (AD) is a heterogenous and multifactorial disease, its pathology partly driven by microglia their activated phenotype. Brain organoids (BOs) are gaining prominence as relevant model of the human brain for study AD; however, BOs commonly devoid microglia. To overcome this limitation, current protocols incorporate through either (1) co-culture (BO co-culture), or (2) molecular manipulation at critical windows BO development to have arise innately innate cultures). It currently unclear whether incorporated into these two differ in function.

Язык: Английский

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Induced pluripotent stem cell-derived macrophages as a platform for modelling human disease

Nature reviews. Immunology, Год журнала: 2024, Номер unknown

Опубликована: Сен. 27, 2024

Язык: Английский

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The organoid modeling approach to understanding the mechanisms underlying neurodegeneration: A comprehensive review

Life Sciences, Год журнала: 2024, Номер 358, С. 123198 - 123198

Опубликована: Окт. 31, 2024

Язык: Английский

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