bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 17, 2023
Summary
The
African
malaria
mosquito
Anopheles
gambiae
exhibits
a
strong
innate
sensory
drive
to
seek
out
human
scent.
To
detect
odorants,
An.
uses
olfactory
neurons
(OSNs)
that
can
be
divided
into
different
classes
by
unique
repertoires
of
chemoreceptor
gene
expression.
We
applied
CRISPR-Cas9-mediated
T2A-In
Frame
Fusions
and
the
QF2/QUAS
system
gain
genetic
access
specific
OSN
subsets
in
expressing
genes
Ir25a
,
Ir76b
Gr22
orco
.
first
optimized
methods
generate
cell-type
QF2
driver
QUAS
responder
lines
map
expression
patterns
these
chemoreceptors
across
appendages.
next
transcuticular
calcium
imaging
record
neurophysiological
responses
select
human-related
odorants
for
each
class.
This
neurogenetic
toolkit
tiling
including
those
responsive
CO
2
stands
support
systematic
efforts
decode
olfaction
this
prolific
disease
vector
at
high-resolution
combat
malaria.
Veterinary World,
Год журнала:
2024,
Номер
unknown, С. 2248 - 2252
Опубликована: Окт. 1, 2024
Background
and
Aim:
Efficient
mosquito
vectors
are
required
to
persist
propagate
arthropod-borne
diseases
that
seriously
affect
impoverished
populations
worldwide.
Mosquito
sensilla
plays
a
crucial
role
in
host-seeking
disease
transmission
humans.
This
study
aimed
distinguish
between
the
several
types
of
found
on
antennae
maxillary
palps
Culex
pipiens
Aedes
aegypti,
matching
this
diversity
with
host
preference
transmission.
Materials
Methods:
Overall,
1300
mosquitoes
were
collected
examined
using
dissection
light
microscopy.
Scanning
electron
microscopy
was
used
identify
describe
diverse
C.
A.
aegypti.
Results:
In
total,
900
400
aegypti
identified.
The
carry
both
trichoidea
chaetica.
antenna
has
long
short
grooved
peg
sensilla,
whereas
lacks
pegs
expresses
only
occasional
pegs.
express
Capitate
species
exclusively
show
campaniform
Conclusion:
lack
long-grooved
presence
few
pegs,
along
limit
range
reduce
its
susceptibility
many
infections,
unlike
pipiens.
Keywords:
pipiens,
microscopy,
Sensilla.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 17, 2023
Summary
The
African
malaria
mosquito
Anopheles
gambiae
exhibits
a
strong
innate
sensory
drive
to
seek
out
human
scent.
To
detect
odorants,
An.
uses
olfactory
neurons
(OSNs)
that
can
be
divided
into
different
classes
by
unique
repertoires
of
chemoreceptor
gene
expression.
We
applied
CRISPR-Cas9-mediated
T2A-In
Frame
Fusions
and
the
QF2/QUAS
system
gain
genetic
access
specific
OSN
subsets
in
expressing
genes
Ir25a
,
Ir76b
Gr22
orco
.
first
optimized
methods
generate
cell-type
QF2
driver
QUAS
responder
lines
map
expression
patterns
these
chemoreceptors
across
appendages.
next
transcuticular
calcium
imaging
record
neurophysiological
responses
select
human-related
odorants
for
each
class.
This
neurogenetic
toolkit
tiling
including
those
responsive
CO
2
stands
support
systematic
efforts
decode
olfaction
this
prolific
disease
vector
at
high-resolution
combat
malaria.
